##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 412534 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.814020177730804 31.0 28.0 31.0 16.0 34.0 2 27.800472203503226 31.0 28.0 31.0 16.0 34.0 3 27.595516975570497 31.0 28.0 31.0 12.0 34.0 4 31.092142708237382 35.0 32.0 37.0 17.0 37.0 5 31.407597919201812 35.0 32.0 37.0 17.0 37.0 6 30.511940833967625 35.0 30.0 37.0 11.0 37.0 7 30.605084671808868 35.0 31.0 37.0 11.0 37.0 8 30.403901254199653 35.0 30.0 37.0 10.0 37.0 9 32.58068183470938 37.0 32.0 39.0 15.0 39.0 10 32.75956890825968 37.0 33.0 39.0 11.0 39.0 11 32.92086470448496 37.0 33.0 39.0 15.0 39.0 12 32.80332772571473 37.0 33.0 39.0 10.0 39.0 13 32.90448544847212 38.0 33.0 39.0 10.0 39.0 14 33.88936669462396 39.0 33.0 40.0 10.0 41.0 15 33.470770894035404 39.0 33.0 40.0 10.0 41.0 16 33.53484076464 39.0 33.0 40.0 10.0 41.0 17 33.77130612264686 39.0 33.0 40.0 10.0 41.0 18 33.81941852065527 39.0 33.0 41.0 8.0 41.0 19 33.232024511919015 39.0 32.0 40.0 2.0 41.0 20 33.508486573227906 39.0 33.0 41.0 2.0 41.0 21 33.49849466953027 39.0 33.0 41.0 2.0 41.0 22 33.50223739134229 39.0 33.0 41.0 2.0 41.0 23 33.501384128338515 39.0 34.0 41.0 2.0 41.0 24 32.23795372017822 38.0 32.0 40.0 2.0 41.0 25 32.80573964812597 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1350227358873255 1101 2 -0.16558358457931988 1101 3 -1.146055317597515 1101 4 -0.6702385386011223 1101 5 -0.20465032952271756 1101 6 -2.2762837977025825 1101 7 -0.6702340394857629 1101 8 -0.34606534178950454 1101 9 -0.029039088351680675 1101 10 -9.381155833819307 1101 11 -4.149196684309636 1101 12 -1.5072324517311984 1101 13 -0.6701642339552052 1101 14 -0.5621707986419295 1101 15 -1.8104987007465994 1101 16 -0.2626938466958819 1101 17 -0.09160955923209002 1101 18 -0.02817180993503854 1101 19 -0.8198874548892263 1101 20 -0.21139388920875746 1101 21 -0.16886454211429935 1101 22 0.17310857356469 1101 23 0.14630189918114667 1101 24 -0.14147787271183176 1101 25 -0.06638003456664876 1102 1 0.04410410340363313 1102 2 -0.2714195447115344 1102 3 -0.5221499589278054 1102 4 -0.3919654621197992 1102 5 -0.01040844282176323 1102 6 -1.2188736725095595 1102 7 -0.13103114001027194 1102 8 0.3325880356144957 1102 9 0.3936972916429653 1102 10 0.35942278510130166 1102 11 0.14470191061883497 1102 12 0.25771605450292867 1102 13 0.177179402385228 1102 14 -0.18699500517851675 1102 15 0.5436558954069994 1102 16 0.29605243643688084 1102 17 0.19326646011476356 1102 18 -0.019215595588249812 1102 19 0.4815814255201687 1102 20 0.41698574810156686 1102 21 0.27895638464586625 1102 22 0.2096429856934776 1102 23 0.10624778925120637 1102 24 -3.3441680550776063 1102 25 -1.9516031742187643 1103 1 0.08358948448497117 1103 2 -0.23993482326590154 1103 3 0.1724129422324836 1103 4 -0.07368608063134019 1103 5 -0.3229082817778881 1103 6 0.35404055165835757 1103 7 0.18969610835545225 1103 8 0.25580489342950585 1103 9 0.21835272217121116 1103 10 0.2828277421605634 1103 11 0.0862751933113799 1103 12 0.16100985263894074 1103 13 0.12305835407979515 1103 14 -0.33721930708553316 1103 15 0.17811464627341422 1103 16 0.28301174773597637 1103 17 0.13561275340013168 1103 18 0.11731095861396312 1103 19 -1.9195131574792654 1103 20 -0.4664494079048538 1103 21 0.06606153727680919 1103 22 -0.08309612580814729 1103 23 0.05053433912456029 1103 24 1.4599742510191263 1103 25 0.7957383135174254 1104 1 -0.4239245445095605 1104 2 -0.31571818854034106 1104 3 -1.3745736629167098 1104 4 -0.6967826924920004 1104 5 -0.3118228880090861 1104 6 -1.801246829009063 1104 7 -0.795768347697237 1104 8 -0.398789650127366 1104 9 -0.3208225592380387 1104 10 -0.0933687734939781 1104 11 -0.08302714651309628 1104 12 -0.18034831224863268 1104 13 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0.3536963760003715 2215 1 0.4421543707056941 2215 2 0.36024788797914553 2215 3 0.4205917619652979 2215 4 0.7105817693081491 2215 5 0.9991926602528132 2215 6 1.4153603437946565 2215 7 0.8844022279957251 2215 8 -0.11377012496026495 2215 9 -0.03807657806844844 2215 10 0.05103361264914241 2215 11 -0.2608666807839555 2215 12 -0.35582805638509285 2215 13 -0.06911828260956554 2215 14 0.008648334041112093 2215 15 -0.18545462976293692 2215 16 0.020711958921701523 2215 17 -0.36133573152025633 2215 18 -1.0134462252599903 2215 19 -0.0205690861032366 2215 20 -3.3054119876927146 2215 21 -1.5481923127101425 2215 22 -0.769093800392298 2215 23 -0.7996376941905794 2215 24 -0.8544053612533737 2215 25 -0.7391500427932343 2216 1 -0.48682786321052873 2216 2 -0.7275696545733012 2216 3 -0.18399063028561358 2216 4 -0.16357940609409027 2216 5 -0.36005711272317953 2216 6 -1.17561923568703 2216 7 -9.417341782063183 2216 8 -10.55289276449271 2216 9 -5.351362721454482 2216 10 -3.1481537578307552 2216 11 -2.0526472475290163 2216 12 -1.6122627316825522 2216 13 -1.5031392852407173 2216 14 -1.3991356788119447 2216 15 -2.4685919851078957 2216 16 -1.3208043285954716 2216 17 -1.542347210785394 2216 18 -1.436141640539283 2216 19 -0.7580082553118004 2216 20 -1.678741905964074 2216 21 -4.496116498334285 2216 22 -2.915576674632643 2216 23 -3.1076172969567786 2216 24 -3.371295947324576 2216 25 -2.7173510812825334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28038.0 3 1.0 4 190.0 5 1217.0 6 1661.0 7 1394.0 8 1056.0 9 1003.0 10 854.0 11 833.0 12 874.0 13 820.0 14 873.0 15 797.0 16 907.0 17 954.0 18 1090.0 19 1399.0 20 1736.0 21 2335.0 22 2803.0 23 3353.0 24 3983.0 25 4408.0 26 5154.0 27 5363.0 28 6009.0 29 7348.0 30 8453.0 31 10426.0 32 13498.0 33 17447.0 34 23584.0 35 32895.0 36 47869.0 37 75778.0 38 96131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.715744771826174 18.792272409235792 12.628760786752702 24.863222032185337 2 13.912473603728245 20.451224544285054 43.26901138377145 22.36729046821525 3 19.478420016554242 24.67285953895708 29.199546578830475 26.6491738656582 4 11.273663170355421 16.877140722484743 39.66267550285753 32.1865206043023 5 12.109772773559104 38.517936632626345 36.50194446868969 12.870346125124863 6 30.23629645434091 37.60377065714951 19.8926895026767 12.26724338583288 7 27.778736393396407 31.582145122523226 22.01165082520603 18.627467658874338 8 23.791921843102944 38.085204627199374 21.618790706717217 16.50408282298046 9 25.995543842720437 16.349587720595544 20.485716294552482 37.16915214213154 10 13.47093470158754 30.993285628723637 35.22704953212787 20.308730137560953 11 33.8535315210039 24.027871050328386 23.763367606449908 18.355229822217805 12 22.04731472681782 26.391609612288836 32.44171725595919 19.119358404934154 13 28.10996113685017 23.028901285659913 25.840479255611882 23.020658321878038 14 19.487134728354025 23.474847684784482 29.082569029764805 27.95544855709669 15 22.913312903663762 31.592932368742606 23.811070812079365 21.682683915514264 16 20.674146951719187 30.40813456754804 28.677550901658822 20.24016757907395 17 20.54627797819931 29.80866208929749 28.337988285038207 21.30707164746499 18 19.849056786747255 29.650183163504035 30.283679174152006 20.217080875596704 19 22.197571992813447 28.448466068757167 28.766575258766792 20.587386679662593 20 21.80594952360172 28.688874342376415 29.773559289160424 19.731616844861442 21 22.19994472378865 27.887818383704182 28.485189369511776 21.427047522995387 22 21.143215958571332 29.266027910043928 29.08686249599969 20.503893635385047 23 20.934190620272314 28.6599557779588 28.976347026649595 21.429506575119284 24 21.216661454204274 28.34391584418859 29.856257258050828 20.583165443556307 25 21.33168150003273 28.40175430515115 29.334425935399093 20.932138259417027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 163.0 2 230.5 3 298.0 4 298.0 5 298.0 6 461.0 7 624.0 8 624.0 9 624.0 10 931.5 11 1239.0 12 1239.0 13 1239.0 14 1991.5 15 2744.0 16 2744.0 17 2744.0 18 4447.0 19 6150.0 20 6150.0 21 6150.0 22 9201.5 23 12253.0 24 12253.0 25 12253.0 26 17017.5 27 21782.0 28 21782.0 29 21782.0 30 27659.5 31 33537.0 32 33537.0 33 33537.0 34 38623.5 35 43710.0 36 43710.0 37 43710.0 38 47127.5 39 50545.0 40 50545.0 41 50545.0 42 52942.0 43 55339.0 44 55339.0 45 55339.0 46 56060.5 47 56782.0 48 56782.0 49 56782.0 50 53425.5 51 50069.0 52 50069.0 53 50069.0 54 42472.5 55 34876.0 56 34876.0 57 34876.0 58 28516.0 59 22156.0 60 22156.0 61 22156.0 62 16961.5 63 11767.0 64 11767.0 65 11767.0 66 8540.5 67 5314.0 68 5314.0 69 5314.0 70 3733.5 71 2153.0 72 2153.0 73 2153.0 74 1432.0 75 711.0 76 711.0 77 711.0 78 464.0 79 217.0 80 217.0 81 217.0 82 142.5 83 68.0 84 68.0 85 68.0 86 47.0 87 26.0 88 26.0 89 26.0 90 16.5 91 7.0 92 7.0 93 7.0 94 4.0 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22252711291675353 2 0.1318679187654836 3 0.13453436565228563 4 0.0702972361065997 5 0.019877149519797157 6 0.02084676656954336 7 0.25719092244518027 8 0.2554940926081244 9 0.0172107026329951 10 0.032724575428934345 11 0.015271468533502693 12 0.015029064271066143 13 0.014786660008629592 14 0.016241085583248896 15 0.015029064271066143 16 0.01745310689543165 17 0.01696829837055855 18 0.014786660008629592 19 0.023513213456345418 20 0.015513872795939245 21 0.01454425574619304 22 0.015998681320812346 23 0.01696829837055855 24 0.014786660008629592 25 0.015271468533502693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 412534.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.12746556077364 #Duplication Level Percentage of deduplicated Percentage of total 1 96.47214733132097 87.91262283508833 2 2.764030830449561 5.03758248621418 3 0.3148321508633879 0.8606956795568302 4 0.12553657182479877 0.4575931850232775 5 0.08153740878417685 0.3715148705447399 6 0.048002302105494006 0.26245968791737545 7 0.03194513978443347 0.20377557378780425 8 0.022599157057519605 0.1647523125169306 9 0.014585790840762487 0.11962495372664081 >10 0.1050724348121189 1.868227436975459 >50 0.01198634739996339 0.760230674702227 >100 0.006659081686872174 1.3012760384089808 >500 7.990898024246595E-4 0.4187094135899069 >1k 2.663632674748865E-4 0.26093485194733324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1075 0.260584582119292 No Hit GGTATCAACGCAGAGTACTTTTTTT 664 0.16095643025786965 No Hit TATCAACGCAGAGTACTTTTTTTTT 533 0.12920147187868153 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 528 0.12798945056649877 No Hit GTCCTACAGTGGACATTTCTAAATT 467 0.11320279055786918 No Hit CACACACACACACACACACACACAC 452 0.1095667266213209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCATCC 45 3.5082507E-5 16.900335 8 TAGGACC 150 0.0 16.49787 4 TAGCACT 45 6.6512084E-4 14.805781 4 GGTCTAG 40 0.0051460373 14.301359 1 CCTAAGC 40 0.005175241 14.28917 3 AGGACCT 220 0.0 14.271794 5 GTGTTAG 75 9.240284E-7 13.98355 1 ACTGTAC 55 1.9434457E-4 13.827548 8 GTTACAC 50 0.0014656379 13.33656 3 CCAGTAC 50 0.0014656379 13.33656 3 TGGCGAG 200 0.0 12.3329 18 TGTTAGG 55 0.0029995327 12.124145 2 TAATACT 55 0.0030187406 12.113821 4 TATAACT 55 0.0030187406 12.113821 4 GTAGCCC 95 9.998803E-7 12.032987 3 GGCGAGA 150 1.4551915E-11 12.01667 19 GGACCTG 255 0.0 11.565278 6 GTAGGAC 310 0.0 11.369878 3 TATGGCG 210 0.0 11.293864 16 CTACACT 135 4.5420165E-9 11.280596 4 >>END_MODULE