FastQCFastQC Report
Thu 26 May 2016
SRR1926986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15750.3817867133375673No Hit
GGTATCAACGCAGAGTACTTTTTTT10720.25985736933198234No Hit
TATCAACGCAGAGTACTTTTTTTTT8780.21283094241929149No Hit
CACACACACACACACACACACACAC6580.15950200468325035No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5860.1420488977878187No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5230.126777429254316No Hit
GTCCTACAGTGGACATTTCTAAATT5140.12459579089238704No Hit
CTGTAGGACGTGGAATATGGCAAGA4730.11465721613248847No Hit
GTCCTACAGTGTGCATTTCTCATTT4380.1061730669472092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1055.456968E-1215.4139434
CTCGAAC508.773868E-515.18273318
TAGCACT851.6569174E-814.5605764
GATTTAA400.005154162614.297961
CCTCGAA551.9735971E-413.80248517
TCGAACT551.9735971E-413.80248519
ATTATAC500.001472310613.3285283
GACTTGG500.001509161713.2848917
GTCCTAC2900.013.147551
CCTGGAC905.234815E-712.6938363
TAGGACT604.0075585E-412.6938364
GCCTCGA604.1200203E-412.65227816
GTGTAGG701.05356194E-412.2553951
AGTACTA550.003013103312.1168445
TGCCTAA550.003088052212.07717510
AGGTCTT550.003088052212.0771757
GGGGCAT802.8878078E-511.8615111
GTATAGT657.7789696E-411.731661
GTAGGAC2400.011.5037893
AGGACCT2000.011.4244535