##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926986_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 412534 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.726691618145413 31.0 30.0 33.0 25.0 34.0 2 30.02672991801888 31.0 30.0 34.0 26.0 34.0 3 30.070326324618094 31.0 30.0 34.0 26.0 34.0 4 33.638165581503586 35.0 35.0 37.0 28.0 37.0 5 33.632323638778864 37.0 35.0 37.0 28.0 37.0 6 33.54023910756447 37.0 35.0 37.0 28.0 37.0 7 33.537126636834785 37.0 35.0 37.0 28.0 37.0 8 33.54698037010283 37.0 35.0 37.0 28.0 37.0 9 35.047159749257034 39.0 35.0 39.0 28.0 39.0 10 34.95627027105645 39.0 35.0 39.0 28.0 39.0 11 34.964448990871055 39.0 35.0 39.0 28.0 39.0 12 34.86436269495363 39.0 35.0 39.0 27.0 39.0 13 34.86902655296291 39.0 35.0 39.0 27.0 39.0 14 35.96531195004533 40.0 36.0 41.0 27.0 41.0 15 35.92392627031954 40.0 36.0 41.0 27.0 41.0 16 35.90237895543155 40.0 36.0 41.0 27.0 41.0 17 35.88998967357842 40.0 36.0 41.0 27.0 41.0 18 35.87407340970684 40.0 36.0 41.0 27.0 41.0 19 35.956338144249926 40.0 36.0 41.0 27.0 41.0 20 35.91943451933659 40.0 36.0 41.0 27.0 41.0 21 35.86977315809121 40.0 36.0 41.0 27.0 41.0 22 35.77721351452244 40.0 36.0 41.0 27.0 41.0 23 35.71155104791362 40.0 36.0 41.0 27.0 41.0 24 35.62271473381588 40.0 36.0 41.0 27.0 41.0 25 35.517288271996975 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10041479419681565 1101 2 0.008391625556924964 1101 3 0.42749340208280984 1101 4 0.20206733225067808 1101 5 0.10533132016074376 1101 6 -0.39229220605964343 1101 7 -0.28497478500696616 1101 8 -0.07944300434613893 1101 9 0.10448977392460534 1101 10 -0.15424526004596117 1101 11 0.14782605341644484 1101 12 -0.09561933628651786 1101 13 -0.09455753879998241 1101 14 -0.1547422644394132 1101 15 -0.2629983015290378 1101 16 -0.19566712729450586 1101 17 0.10080232094622943 1101 18 -0.13539162596049437 1101 19 0.0701983118032885 1101 20 -0.10459202765028408 1101 21 0.12662776757487393 1101 22 0.07968204083836383 1101 23 0.00885996116375054 1101 24 0.0964291083906943 1101 25 0.17049184090517855 1102 1 -0.5518676494280363 1102 2 -0.3579555713189926 1102 3 -0.0701903355861262 1102 4 -0.3383045458998879 1102 5 -0.5553177437629913 1102 6 -0.442895199972277 1102 7 -0.22955294450363795 1102 8 -0.2194014009566061 1102 9 -0.36843867079775805 1102 10 -0.3326268116226814 1102 11 -0.3484360224140275 1102 12 -0.5038583388261131 1102 13 -0.6724235146291804 1102 14 -0.3967463737312613 1102 15 -0.3269705319046139 1102 16 -0.4351061196246775 1102 17 -0.24122093100950792 1102 18 -0.2884716805808978 1102 19 -0.3129535916155959 1102 20 -0.07334609546636273 1102 21 -0.16859039498463346 1102 22 -0.2625453995322431 1102 23 -0.3878832336192346 1102 24 -0.29688882573302067 1102 25 -0.26889610432127853 1103 1 -0.7599234504860313 1103 2 -0.7621628103055222 1103 3 -0.4119718157983421 1103 4 -0.3731364032124418 1103 5 -0.42811885071416356 1103 6 -0.5498025649595348 1103 7 -0.4814796734062483 1103 8 -0.3832873767407605 1103 9 -0.5599494684673871 1103 10 -0.5024495087597103 1103 11 -0.34353255855355513 1103 12 -0.4267711458079404 1103 13 -0.6735418032867955 1103 14 -0.7701723309116275 1103 15 -0.5441169375341914 1103 16 -0.7562044130749541 1103 17 -0.5033462463334146 1103 18 -0.5733621578634001 1103 19 -0.581314525721595 1103 20 -0.6879101303755206 1103 21 -0.6249088547352599 1103 22 -0.5685184523829747 1103 23 -0.7202288311950724 1103 24 -0.6898333003821406 1103 25 -0.3791119546458148 1104 1 -0.13603065012264182 1104 2 -0.16393177783787394 1104 3 -0.25637055312503776 1104 4 -0.05091228125269254 1104 5 -0.06654719502486017 1104 6 -0.21756994521865636 1104 7 -0.23875191118079897 1104 8 -0.15044817364379526 1104 9 -0.11359139289582743 1104 10 -0.2698808056027673 1104 11 -0.2825577890526816 1104 12 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23 0.11044473142190014 1105 24 0.37209441288585765 1105 25 0.4001589394798799 1106 1 0.8058013088700022 1106 2 0.5913537786887204 1106 3 0.8721480386619724 1106 4 0.7462220118236829 1106 5 1.0640523146698158 1106 6 1.0789621086798746 1106 7 0.8329683894129261 1106 8 0.951942503042666 1106 9 0.8344933362718265 1106 10 1.023633792123995 1106 11 0.884276235833191 1106 12 0.8705187108101669 1106 13 1.044570546008444 1106 14 0.8313817789475166 1106 15 0.7445268498707236 1106 16 0.9940020657724347 1106 17 0.8777281381363977 1106 18 0.793599738418493 1106 19 0.768796712432227 1106 20 0.9405048313421815 1106 21 0.8469343751147633 1106 22 0.7594294827045402 1106 23 0.6712575436812216 1106 24 1.0707802084189453 1106 25 1.0935819928577146 1107 1 0.4771561630641692 1107 2 0.2625406261626182 1107 3 0.3929688714952846 1107 4 0.6328486768902906 1107 5 0.6820242335465707 1107 6 0.6979924698943236 1107 7 0.42349281038976727 1107 8 0.2787035734854797 1107 9 0.4019216334037097 1107 10 0.3389104031884429 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1108 23 0.4057709242164478 1108 24 0.4131947049988085 1108 25 0.7001102539881572 1109 1 0.03513048203693003 1109 2 0.04065175060759785 1109 3 0.14983914630627737 1109 4 0.15339829887516743 1109 5 0.45094779048884703 1109 6 0.4393365236564719 1109 7 0.3821103550915552 1109 8 0.36067885249664045 1109 9 0.11530456872112183 1109 10 0.22488184204599548 1109 11 0.2087692155523797 1109 12 0.156010130466953 1109 13 0.22709784710048808 1109 14 0.0669512017244287 1109 15 0.10178114004232697 1109 16 0.193690053291931 1109 17 0.20066105545308943 1109 18 0.1820552766400212 1109 19 0.09172962974891874 1109 20 0.15891356769163423 1109 21 0.4459983376732666 1109 22 0.33665257162809326 1109 23 0.6681374188762348 1109 24 0.4951171818978821 1109 25 0.31043066680467035 1110 1 0.25879844716044076 1110 2 0.17110598718196712 1110 3 0.5403261154782975 1110 4 0.38807283923101465 1110 5 0.263864724344117 1110 6 0.5147267785304734 1110 7 0.3331813427163368 1110 8 0.3681561454533906 1110 9 0.20874560697615152 1110 10 0.13699912428329952 1110 11 0.24345428393671398 1110 12 0.4514170387678291 1110 13 0.5378756769866939 1110 14 0.38380418259212945 1110 15 0.38756447321614473 1110 16 0.40474831157318647 1110 17 0.31556730814492084 1110 18 0.2774966403068362 1110 19 0.24725014504805642 1110 20 0.39321484158308095 1110 21 0.3339849000805444 1110 22 0.5751339513740845 1110 23 0.4222409572508994 1110 24 0.2857194808364696 1110 25 0.3128521133557811 1111 1 -0.047552825295365864 1111 2 -0.2620003554766477 1111 3 -0.03580827959075705 1111 4 -0.03536925182685735 1111 5 -0.16371679609461864 1111 6 -0.04272275871482378 1111 7 -0.36827966050907435 1111 8 -0.4926752816687241 1111 9 -0.5056002674723246 1111 10 -0.5832304824469858 1111 11 -0.15784544903419828 1111 12 -0.26832684301198384 1111 13 -0.3080035569556685 1111 14 -0.4775105767467096 1111 15 -0.3116353966191383 1111 16 -0.5925813975661001 1111 17 -0.5949707698199163 1111 18 -0.471610870005847 1111 19 -0.468332772747182 1111 20 -0.35590702513207617 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25 0.03766011153801685 2213 1 0.32508515714701147 2213 2 0.4770376675827386 2213 3 0.4343889898384816 2213 4 0.46758240470094137 2213 5 0.5528526359134318 2213 6 0.5598859610185798 2213 7 0.37100760328917204 2213 8 0.4372834551422855 2213 9 0.39695121920252774 2213 10 0.3412772670937656 2213 11 0.49044125166459906 2213 12 0.513619819469902 2213 13 0.4602810205812773 2213 14 0.4585037869047568 2213 15 0.18590912054616382 2213 16 0.561407838926435 2213 17 0.2532646980603346 2213 18 0.4195447762200004 2213 19 0.6436363214011962 2213 20 0.5347547551852898 2213 21 0.6296795310748493 2213 22 0.4449292103859577 2213 23 0.4081377881217705 2213 24 0.46573908369703076 2213 25 0.5441930942248447 2214 1 0.6771561630641685 2214 2 0.7891678450975306 2214 3 0.711016208773394 2214 4 0.6007184993754962 2214 5 0.9566987897595851 2214 6 0.7921344817286382 2214 7 1.0100608577270407 2214 8 0.9831414433079644 2214 9 0.9990222251196883 2214 10 0.9167210540760138 2214 11 0.9120388848006868 2214 12 0.9085902336710845 2214 13 1.0179733282735839 2214 14 0.9451901774373042 2214 15 0.9773292388893609 2214 16 0.8617036969146028 2214 17 0.8272291951017579 2214 18 1.0393672614271026 2214 19 0.9705593983242764 2214 20 0.9962932167237497 2214 21 0.9669576375837252 2214 22 1.1993670802084395 2214 23 1.0590253620862669 2214 24 1.0589343499692205 2214 25 1.0708203131597571 2215 1 0.3910482411353513 2215 2 0.4115647363807611 2215 3 0.20157966368816815 2215 4 0.1627912716090023 2215 5 0.36202423354657043 2215 6 0.1115860405028215 2215 7 0.06852151303040444 2215 8 0.2327793484567806 2215 9 -0.11823910138388527 2215 10 0.22926631593241353 2215 11 -0.034945130036369676 2215 12 0.06788988923939598 2215 13 -0.014125055546955423 2215 14 0.20264403242273232 2215 15 0.22601333321065198 2215 16 0.06881753600318063 2215 17 0.14864578081225943 2215 18 0.340210677482915 2215 19 0.15951550536304637 2215 20 0.24808161202535217 2215 21 0.12117471786280021 2215 22 0.0963820055815745 2215 23 0.17500169344721428 2215 24 0.13089849374737383 2215 25 0.32559819887908503 2216 1 -0.2191596895699348 2216 2 0.011010564960169944 2216 3 -1.6785539894191501 2216 4 -1.0681305622792792 2216 5 -0.3134827867342409 2216 6 0.022799862190012732 2216 7 0.046696938554894984 2216 8 0.03618587277745178 2216 9 0.012340450156379745 2216 10 -0.006007393523425719 2216 11 -0.0018392087845882088 2216 12 0.16381044247943777 2216 13 -0.1629177535235371 2216 14 -0.04069310404780424 2216 15 -0.23363227649433327 2216 16 -0.1822849857096287 2216 17 -0.1612734219260048 2216 18 0.1648945902125618 2216 19 0.06520856890648474 2216 20 0.16827394210661595 2216 21 0.11058492113660634 2216 22 0.21808782903839585 2216 23 0.15019670124753048 2216 24 -0.008782974108356711 2216 25 -0.1731886779691223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11015.0 3 9.0 4 190.0 5 1256.0 6 1745.0 7 1251.0 8 1017.0 9 858.0 10 762.0 11 677.0 12 589.0 13 573.0 14 573.0 15 541.0 16 551.0 17 597.0 18 635.0 19 815.0 20 1134.0 21 1359.0 22 1665.0 23 2138.0 24 2390.0 25 2878.0 26 3393.0 27 3761.0 28 4319.0 29 5309.0 30 6607.0 31 8353.0 32 11226.0 33 14681.0 34 19733.0 35 27323.0 36 40871.0 37 70809.0 38 160931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27653719405361 19.609689333676307 10.674089137598827 27.439684334671256 2 13.672058902041867 21.18017637608779 41.34378776627499 23.803976955595356 3 18.985451380555133 24.93120861910546 28.6933395397099 27.39000046062951 4 11.277858309860521 17.871496652397138 38.34714229615013 32.50350274159221 5 12.20809444734003 39.30182664452552 35.34648064871941 13.143598259415038 6 30.589105310145687 38.71816016794744 18.247255407085223 12.445479114821653 7 28.209504920727674 32.362361464362 20.822473141482742 18.605660473427584 8 24.496232654209052 39.089727800781596 19.540742608341496 16.873296936667863 9 25.917162632274334 16.58638286524685 19.88581679777937 37.61063770469945 10 13.604673712789683 31.666343450014544 34.13289052651993 20.596092310675846 11 33.73170365127002 24.202087686720937 23.184425255870103 18.881783406138943 12 22.130322959113176 26.804176191249585 31.787825825578324 19.277675024058915 13 28.274723596529718 22.895006678285995 25.851875374214302 22.97839435096999 14 19.726858877086496 23.828823806037803 28.503832411389123 27.940484905486578 15 22.54112468062619 31.729151617110197 23.652327368456778 22.07739633380684 16 20.649749835162705 30.687129116084243 28.213842454330372 20.449278594422683 17 20.30916314573622 30.021113462438155 28.17471571971212 21.495007672113502 18 19.85184143737077 29.63922711262911 30.189973601983848 20.318957848016268 19 21.567192037504785 28.422142174948007 29.090208322223138 20.92045746532407 20 21.66078021181541 28.69446754046382 29.710638524806743 19.934113722914027 21 22.015399385188275 27.780471484954568 28.485710960909998 21.71841816894716 22 21.00481414865199 29.334794223021614 29.058938172368826 20.601453455957568 23 21.12854275547109 28.57014728554391 28.888910436038902 21.4123995229461 24 20.709080948479397 28.3591170667145 30.141515608410458 20.790286376395642 25 21.13865286481376 28.459048981224473 29.2883722621544 21.113925891807373 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 271.0 1 271.0 2 270.0 3 269.0 4 269.0 5 269.0 6 437.5 7 606.0 8 606.0 9 606.0 10 865.0 11 1124.0 12 1124.0 13 1124.0 14 1775.0 15 2426.0 16 2426.0 17 2426.0 18 3953.0 19 5480.0 20 5480.0 21 5480.0 22 8661.0 23 11842.0 24 11842.0 25 11842.0 26 16481.0 27 21120.0 28 21120.0 29 21120.0 30 27159.5 31 33199.0 32 33199.0 33 33199.0 34 37762.5 35 42326.0 36 42326.0 37 42326.0 38 46053.5 39 49781.0 40 49781.0 41 49781.0 42 52370.5 43 54960.0 44 54960.0 45 54960.0 46 56368.0 47 57776.0 48 57776.0 49 57776.0 50 54556.0 51 51336.0 52 51336.0 53 51336.0 54 43672.0 55 36008.0 56 36008.0 57 36008.0 58 29397.5 59 22787.0 60 22787.0 61 22787.0 62 17546.0 63 12305.0 64 12305.0 65 12305.0 66 9003.0 67 5701.0 68 5701.0 69 5701.0 70 3929.5 71 2158.0 72 2158.0 73 2158.0 74 1442.0 75 726.0 76 726.0 77 726.0 78 477.5 79 229.0 80 229.0 81 229.0 82 153.0 83 77.0 84 77.0 85 77.0 86 48.5 87 20.0 88 20.0 89 20.0 90 13.5 91 7.0 92 7.0 93 7.0 94 3.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1095667266213209 2 0.11150596072081333 3 0.013332234434010288 4 0.0 5 0.0070297236106599695 6 0.35221339332030815 7 0.023028404931472315 8 0.010180979022335128 9 0.009453766235025477 10 0.0033936596741117094 11 0.0048480852487310135 12 0.0012120213121827534 13 7.27212787309652E-4 14 0.0 15 0.0019392340994924056 16 0.001454425574619304 17 2.424042624365507E-4 18 7.27212787309652E-4 19 0.0 20 0.0012120213121827534 21 0.012120213121827534 22 0.0 23 4.848085248731014E-4 24 0.0 25 0.0070297236106599695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 412534.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.11818193137333 #Duplication Level Percentage of deduplicated Percentage of total 1 95.55957079609153 85.16095215490034 2 3.5390404539710247 6.307857020789596 3 0.3818918654380896 1.0210052622666965 4 0.15356304666370024 0.5474103812204644 5 0.08587178785638801 0.3826368806478941 6 0.05786609762284853 0.3094152849366975 7 0.03588854685526496 0.22388254335300836 8 0.026586023099109922 0.18954384347025377 9 0.016604067763818453 0.13317518985991417 >10 0.11916687366682413 2.037396440782651 >50 0.013608205240504925 0.851827524954802 >100 0.008437087043600584 1.4266655488632665 >500 0.001360820490903317 0.7662081721963708 >1k 5.443281963613268E-4 0.642023751758086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1575 0.3817867133375673 No Hit GGTATCAACGCAGAGTACTTTTTTT 1072 0.25985736933198234 No Hit TATCAACGCAGAGTACTTTTTTTTT 878 0.21283094241929149 No Hit CACACACACACACACACACACACAC 658 0.15950200468325035 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 586 0.1420488977878187 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 523 0.126777429254316 No Hit GTCCTACAGTGGACATTTCTAAATT 514 0.12459579089238704 No Hit CTGTAGGACGTGGAATATGGCAAGA 473 0.11465721613248847 No Hit GTCCTACAGTGTGCATTTCTCATTT 438 0.1061730669472092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 105 5.456968E-12 15.413943 4 CTCGAAC 50 8.773868E-5 15.182733 18 TAGCACT 85 1.6569174E-8 14.560576 4 GATTTAA 40 0.0051541626 14.29796 1 CCTCGAA 55 1.9735971E-4 13.802485 17 TCGAACT 55 1.9735971E-4 13.802485 19 ATTATAC 50 0.0014723106 13.328528 3 GACTTGG 50 0.0015091617 13.284891 7 GTCCTAC 290 0.0 13.14755 1 CCTGGAC 90 5.234815E-7 12.693836 3 TAGGACT 60 4.0075585E-4 12.693836 4 GCCTCGA 60 4.1200203E-4 12.652278 16 GTGTAGG 70 1.05356194E-4 12.255395 1 AGTACTA 55 0.0030131033 12.116844 5 TGCCTAA 55 0.0030880522 12.077175 10 AGGTCTT 55 0.0030880522 12.077175 7 GGGGCAT 80 2.8878078E-5 11.86151 11 GTATAGT 65 7.7789696E-4 11.73166 1 GTAGGAC 240 0.0 11.503789 3 AGGACCT 200 0.0 11.424453 5 >>END_MODULE