FastQCFastQC Report
Thu 26 May 2016
SRR1926985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550225
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13380.2431732473079195No Hit
GGTATCAACGCAGAGTACTTTTTTT8220.1493934299604707No Hit
TATCAACGCAGAGTACTTTTTTTTT6970.12667545095188332No Hit
GTCCTACAGTGGACATTTCTAAATT6640.12067790449361625No Hit
CACACACACACACACACACACACAC6190.11249943205052478No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6140.11159071289018128No Hit
GTCCTACAGTGTGCATTTCTCATTT6060.11013676223363171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC508.844162E-515.17025618
TAGGACC1550.013.5164654
GTGTTAG500.001453268313.3528771
TCGAACT500.001519665113.27397319
CCCTAGT603.9500295E-412.7170261
ACCTAGG701.0488286E-412.2628461
TGTAGAA1950.012.2189692
GGCGAGG1251.4442776E-912.13620419
GACGTGG1103.677269E-812.1179237
TCCAACG550.003109393212.06724818
TGTAGGA4350.012.0504322
GTAGGAC3750.011.9452643
TGGCGAG3050.011.81290318
GAACAGT1302.5065674E-911.71843056
TTTAGAA907.1726245E-611.648752
GGTATCA4450.011.5739221
GTGTAGG1251.7167622E-811.4453221
CTAGGAC752.0078609E-411.43695453
ATTTCTC4000.011.37769115
GGACCTG2950.011.2963696
Produced by FastQC (version 0.11.5)