##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926985_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550225 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.01703303194148 31.0 28.0 31.0 16.0 34.0 2 28.03367167976737 31.0 28.0 31.0 16.0 34.0 3 27.815964378208914 31.0 28.0 31.0 16.0 34.0 4 31.348602844290973 35.0 32.0 37.0 17.0 37.0 5 31.62206551865146 35.0 32.0 37.0 17.0 37.0 6 30.788266618201646 35.0 31.0 37.0 11.0 37.0 7 30.84862192739334 35.0 32.0 37.0 11.0 37.0 8 30.636681357626426 35.0 31.0 37.0 10.0 37.0 9 32.78096233359081 37.0 32.0 39.0 17.0 39.0 10 32.972060520696076 37.0 33.0 39.0 17.0 39.0 11 33.12358580580671 38.0 33.0 39.0 17.0 39.0 12 32.997473760734245 38.0 33.0 39.0 13.0 39.0 13 33.08930710164024 38.0 33.0 39.0 15.0 39.0 14 34.08651369894134 39.0 34.0 41.0 13.0 41.0 15 33.663699395701755 39.0 33.0 41.0 10.0 41.0 16 33.750036803125994 39.0 33.0 41.0 10.0 41.0 17 33.97887046208369 39.0 34.0 41.0 11.0 41.0 18 34.039912762960604 39.0 34.0 41.0 10.0 41.0 19 33.46357399245763 39.0 33.0 41.0 8.0 41.0 20 33.71383161433959 39.0 33.0 41.0 8.0 41.0 21 33.710732882002816 39.0 34.0 41.0 2.0 41.0 22 33.72233540824208 39.0 34.0 41.0 2.0 41.0 23 33.71472397655504 39.0 34.0 41.0 2.0 41.0 24 32.48120677904494 39.0 32.0 41.0 2.0 41.0 25 33.0228597391976 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.40692095206371803 1101 2 -0.26879144016029244 1101 3 -1.1359226134810854 1101 4 -0.8251746663567694 1101 5 -0.7323651829180129 1101 6 -2.0376308575443502 1101 7 -0.6083723076518872 1101 8 -0.2933361558270562 1101 9 -0.7479609159651304 1101 10 -9.69969842647917 1101 11 -4.347213954898127 1101 12 -1.9284724011517262 1101 13 -0.9811900381114853 1101 14 -1.1345213851881795 1101 15 -2.2257103660435256 1101 16 -0.5687721176549516 1101 17 -0.6463108413415242 1101 18 -0.6029711257139141 1101 19 -1.0370264379112015 1101 20 -0.5010579046121038 1101 21 -0.6067043085722545 1101 22 -0.5130599108484049 1101 23 -0.5145235497145038 1101 24 -0.5544794681534491 1101 25 -0.41088745680775673 1102 1 -0.24827527769243218 1102 2 -0.7132462235997039 1102 3 -0.5893723225417666 1102 4 -0.5383544035808718 1102 5 -0.2874730599914983 1102 6 -1.3499905056876749 1102 7 -0.483048180159674 1102 8 0.020503002496038647 1102 9 -0.11185218282286513 1102 10 -0.1341192153031301 1102 11 -0.5359833250051977 1102 12 -0.2125197454774792 1102 13 -0.25394099653631486 1102 14 -0.7306058010142849 1102 15 0.1270613117272248 1102 16 0.030218589656371364 1102 17 -0.4296789136979484 1102 18 -0.447234844526335 1102 19 -0.05961610801455208 1102 20 -0.12519097988412398 1102 21 -0.4542961498645468 1102 22 -0.5000358788885038 1102 23 -0.8942769026505673 1102 24 -4.060980628188684 1102 25 -2.6014372018481424 1103 1 -0.3959006518343351 1103 2 -0.5603466431318402 1103 3 -0.3415262393566536 1103 4 -0.35493316680877385 1103 5 -0.585898812667903 1103 6 0.046770989831870224 1103 7 -0.1600099783835205 1103 8 -0.35359266069456297 1103 9 -0.22434906176272307 1103 10 -0.2503613664148432 1103 11 -0.40038751847701803 1103 12 -0.6052153858573703 1103 13 -0.40595435004993874 1103 14 -0.3840278700690902 1103 15 -0.26193683567312576 1103 16 -0.2696081722398773 1103 17 -0.25180776955583184 1103 18 -0.4660752589414727 1103 19 -1.9112016065673032 1103 20 -0.8139295783816962 1103 21 -0.45661575258827014 1103 22 -0.31789697398758676 1103 23 -0.19572815708555424 1103 24 1.0357984377435159 1103 25 0.4309570905420159 1104 1 0.2086359497739565 1104 2 0.30198016307812736 1104 3 -1.108862342878382 1104 4 -0.04120705056120855 1104 5 0.3769636342507532 1104 6 -1.3263074010262024 1104 7 0.3273044744890683 1104 8 0.6708144931421174 1104 9 0.7111824814389891 1104 10 0.8394462560124296 1104 11 0.514740112403743 1104 12 0.7297402257506604 1104 13 0.4097794685985079 1104 14 0.5349357571444386 1104 15 0.9659833792521368 1104 16 0.4044659356211042 1104 17 0.4652209829319389 1104 18 0.2493631438974404 1104 19 -0.40845802536140496 1104 20 0.34272594367413944 1104 21 0.6515477888740904 1104 22 0.7440080702791718 1104 23 0.9762880748196565 1104 24 2.064170822827073 1104 25 1.437444683230062 1105 1 0.12657490589677423 1105 2 -0.1434808602747637 1105 3 0.554637511861646 1105 4 0.10525836238904418 1105 5 0.10737413459547795 1105 6 0.011772536764947716 1105 7 0.13547747983839287 1105 8 0.5381249288786734 1105 9 -0.24824445713121435 1105 10 0.097044942457579 1105 11 -0.09311722351392149 1105 12 -0.05603961794671619 1105 13 -0.11980659106936997 1105 14 -0.2092534826328034 1105 15 0.41155425094205356 1105 16 -0.14242412787412917 1105 17 -0.13557281584036218 1105 18 -0.13077569861906824 1105 19 -0.8228695490301092 1105 20 -0.17375347493908322 1105 21 -0.006747278212891672 1105 22 -0.1411021321742041 1105 23 -0.06245369920208077 1105 24 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1107 11 -0.3411727324389915 1107 12 0.005465054427517657 1107 13 -0.3449604716580339 1107 14 -0.06300397225285792 1107 15 -1.5871531521131743 1107 16 -0.48904574949575164 1107 17 -0.45142811213043643 1107 18 -0.7258389295518697 1107 19 0.37143127353158434 1107 20 0.21797194613417048 1107 21 0.03595738297003237 1107 22 0.11668422561215408 1107 23 -0.3667623556846493 1107 24 -4.235609532119334 1107 25 -2.0611539813280153 1108 1 0.38923246985817883 1108 2 0.21895361519304402 1108 3 0.742334873877919 1108 4 0.6634005439394741 1108 5 0.6529413648565345 1108 6 -2.3064994666574776 1108 7 -0.8446776537336156 1108 8 0.5846992795979169 1108 9 0.30429240320331985 1108 10 0.6273086037140914 1108 11 0.6030957567536532 1108 12 0.6781299430200605 1108 13 0.6422360055326095 1108 14 0.5697751020373047 1108 15 -0.5961379829183073 1108 16 0.5568410649733266 1108 17 0.3856864069560757 1108 18 0.4870368275187573 1108 19 0.5295993015362512 1108 20 -0.574336494161436 1108 21 0.04308495978839488 1108 22 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-0.7918375450760919 2215 1 0.6181910801721457 2215 2 0.455992769730063 2215 3 0.3927644685322882 2215 4 1.0516634240780647 2215 5 0.7998956354820628 2215 6 1.5543853024161436 2215 7 1.1260929058791902 2215 8 -0.09268024845788503 2215 9 0.16821322914879033 2215 10 0.07167569551907604 2215 11 0.0879518212895718 2215 12 -0.16381889991451004 2215 13 0.16121567356245947 2215 14 0.17199250785978393 2215 15 0.11538116678861599 2215 16 0.5506321808104815 2215 17 -0.2226832033960875 2215 18 -0.7638851406445397 2215 19 0.4390659092232383 2215 20 -3.6159861672062057 2215 21 -1.6653537750729086 2215 22 -0.7515362199050202 2215 23 -0.5433551047166816 2215 24 -0.5552228473503895 2215 25 -0.30139006520190037 2216 1 -1.14831770275012 2216 2 -1.73433199464926 2216 3 -1.0837096120979623 2216 4 -1.2301415332670302 2216 5 -1.5054757710561617 2216 6 -2.1558932798725436 2216 7 -10.652766519166928 2216 8 -11.413585531356052 2216 9 -6.48835128775762 2216 10 -4.176422688984072 2216 11 -3.318746334493923 2216 12 -2.7848234261099236 2216 13 -2.7792175354794146 2216 14 -2.7965793526124685 2216 15 -3.597941533855913 2216 16 -2.7899793594542572 2216 17 -2.9825188056692653 2216 18 -2.6621410437585453 2216 19 -2.1528425853895783 2216 20 -3.154192645843704 2216 21 -5.675695452437989 2216 22 -4.152709174901581 2216 23 -4.006250599114804 2216 24 -4.287763256679696 2216 25 -3.9589459196801187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35283.0 3 3.0 4 241.0 5 1558.0 6 2200.0 7 1776.0 8 1472.0 9 1293.0 10 1190.0 11 1180.0 12 1123.0 13 1065.0 14 1060.0 15 1098.0 16 1142.0 17 1254.0 18 1391.0 19 1731.0 20 2260.0 21 2943.0 22 3795.0 23 4556.0 24 5138.0 25 5857.0 26 6713.0 27 7184.0 28 8030.0 29 9361.0 30 11435.0 31 13411.0 32 17240.0 33 22441.0 34 30684.0 35 43032.0 36 62102.0 37 100395.0 38 137588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4026810061697 18.966506549502434 12.815020046523234 24.81579239780463 2 13.716055628550944 20.654519910755557 43.19856888600951 22.430855574683985 3 19.167948169649762 24.743168603328574 29.578969398618703 26.509913828402958 4 11.264142467686701 17.039161518302233 39.794417032978025 31.902278981033046 5 12.024534077936053 38.60608770896505 36.69458926870414 12.674788944394756 6 30.09492888514005 37.94464966115005 19.95887990459847 12.001541549111428 7 27.40668352528702 31.623951881826784 22.34654492481991 18.622819668066285 8 23.75976970211866 37.98383612241958 21.79461702403069 16.46177715143107 9 25.634877641763588 16.32376305657455 20.746547029118023 37.29481227254384 10 13.184161915289739 31.051642134047007 35.66241709332092 20.10177885734233 11 33.809286633558976 23.87807112578255 23.89279507892766 18.41984716173081 12 21.799805495214635 26.46882924479427 32.59245794061242 19.138907319378674 13 28.23755882711146 23.11803231605678 26.01898827365945 22.62542058317231 14 19.42477168285731 23.6210080856262 29.215868768541682 27.738351462974816 15 22.6133000807079 31.820726661964766 23.964430354896642 21.60154290243069 16 20.443444227717357 30.41775812893222 28.88295464667526 20.25584299667517 17 20.24824035832703 29.954082077831423 28.557457390495028 21.24022017334652 18 19.652919662917263 29.729955719718177 30.645735932467115 19.97138868489744 19 22.13088081036517 28.344056152547026 28.862169202996697 20.66289383409111 20 21.762564099767502 28.735964400429726 29.976241504110927 19.52522999569184 21 22.137634924616723 28.031054929633793 28.39115687065778 21.440153275091706 22 20.995140998303985 29.461219785753105 29.19436552126563 20.349273694677276 23 20.9494083921245 28.861266180825325 28.89671357235176 21.292611854698414 24 21.247496028182635 28.411309409117187 29.9198729018443 20.421321660855877 25 21.200052351643077 28.510922952196406 29.417624326063486 20.87140037009703 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 270.0 3 349.0 4 349.0 5 349.0 6 580.5 7 812.0 8 812.0 9 812.0 10 1241.5 11 1671.0 12 1671.0 13 1671.0 14 2689.0 15 3707.0 16 3707.0 17 3707.0 18 6054.5 19 8402.0 20 8402.0 21 8402.0 22 12777.5 23 17153.0 24 17153.0 25 17153.0 26 23546.5 27 29940.0 28 29940.0 29 29940.0 30 38146.0 31 46352.0 32 46352.0 33 46352.0 34 52839.5 35 59327.0 36 59327.0 37 59327.0 38 63968.5 39 68610.0 40 68610.0 41 68610.0 42 71191.0 43 73772.0 44 73772.0 45 73772.0 46 74407.0 47 75042.0 48 75042.0 49 75042.0 50 70142.5 51 65243.0 52 65243.0 53 65243.0 54 55402.0 55 45561.0 56 45561.0 57 45561.0 58 36929.5 59 28298.0 60 28298.0 61 28298.0 62 21657.5 63 15017.0 64 15017.0 65 15017.0 66 10886.5 67 6756.0 68 6756.0 69 6756.0 70 4711.0 71 2666.0 72 2666.0 73 2666.0 74 1785.0 75 904.0 76 904.0 77 904.0 78 614.0 79 324.0 80 324.0 81 324.0 82 213.0 83 102.0 84 102.0 85 102.0 86 61.0 87 20.0 88 20.0 89 20.0 90 11.5 91 3.0 92 3.0 93 3.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22754327775001137 2 0.1314007905856695 3 0.13449043573083738 4 0.06797219319369348 5 0.02417192966513699 6 0.02344495433686219 7 0.31659775546367397 8 0.31223590349402514 9 0.0216275160161752 10 0.03798446090235812 11 0.018356127038938616 12 0.019991821527556908 13 0.01744740787859512 14 0.020537053023763005 15 0.01690217638238902 16 0.022172747512381297 17 0.020537053023763005 18 0.01690217638238902 19 0.027625062474442273 20 0.019264846199282114 21 0.018719614703076013 22 0.020355309191694305 23 0.020718796855831705 24 0.01799263937480122 25 0.01799263937480122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 550225.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.33621652086246 #Duplication Level Percentage of deduplicated Percentage of total 1 96.07330698920626 86.78899062052226 2 3.0963314652135128 5.594217393237744 3 0.35156935961928476 0.9527833737800602 4 0.1360673959017818 0.491672549504531 5 0.08023372868602978 0.36240057434286593 6 0.05092338463389032 0.2760135540157365 7 0.03576500121348525 0.22616124254432185 8 0.027257156745628536 0.19698467308129486 9 0.014605770629248074 0.11874870522160269 >10 0.1140089023217104 1.9450407286626419 >50 0.011072704273794583 0.7191000585271256 >100 0.00724757036348869 1.2543307323267325 >500 0.0014092497928659292 0.8302189057553542 >1k 2.0132139898084702E-4 0.24333688847769197 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1338 0.2431732473079195 No Hit GGTATCAACGCAGAGTACTTTTTTT 822 0.1493934299604707 No Hit TATCAACGCAGAGTACTTTTTTTTT 697 0.12667545095188332 No Hit GTCCTACAGTGGACATTTCTAAATT 664 0.12067790449361625 No Hit CACACACACACACACACACACACAC 619 0.11249943205052478 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 614 0.11159071289018128 No Hit GTCCTACAGTGTGCATTTCTCATTT 606 0.11013676223363171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8174383206869918E-4 0.0 10 0.0 0.0 0.0 1.8174383206869918E-4 0.0 11 0.0 0.0 0.0 1.8174383206869918E-4 0.0 12 0.0 0.0 0.0 1.8174383206869918E-4 0.0 13 0.0 0.0 0.0 1.8174383206869918E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAC 50 8.844162E-5 15.170256 18 TAGGACC 155 0.0 13.516465 4 GTGTTAG 50 0.0014532683 13.352877 1 TCGAACT 50 0.0015196651 13.273973 19 CCCTAGT 60 3.9500295E-4 12.717026 1 ACCTAGG 70 1.0488286E-4 12.262846 1 TGTAGAA 195 0.0 12.218969 2 GGCGAGG 125 1.4442776E-9 12.136204 19 GACGTGG 110 3.677269E-8 12.117923 7 TCCAACG 55 0.0031093932 12.067248 18 TGTAGGA 435 0.0 12.050432 2 GTAGGAC 375 0.0 11.945264 3 TGGCGAG 305 0.0 11.812903 18 GAACAGT 130 2.5065674E-9 11.7184305 6 TTTAGAA 90 7.1726245E-6 11.64875 2 GGTATCA 445 0.0 11.573922 1 GTGTAGG 125 1.7167622E-8 11.445322 1 CTAGGAC 75 2.0078609E-4 11.4369545 3 ATTTCTC 400 0.0 11.377691 15 GGACCTG 295 0.0 11.296369 6 >>END_MODULE