FastQCFastQC Report
Thu 26 May 2016
SRR1926985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550225
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21510.3909309827797719No Hit
GGTATCAACGCAGAGTACTTTTTTT13760.25007951292653No Hit
TATCAACGCAGAGTACTTTTTTTTT10570.192103230496615No Hit
CACACACACACACACACACACACAC8490.1543005134263256No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7520.13667136171566177No Hit
GTCCTACAGTGGACATTTCTAAATT6920.12576673179153983No Hit
CTGTAGGACGTGGAATATGGCAAGA6670.12122313598982234No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6440.11704302785224227No Hit
GTCCTACAGTGTGCATTTCTCATTT6360.11558907719569267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCGT307.787172E-418.9733714
TTTTCCG250.006076257718.97336813
GTCCTAG903.437708E-813.7768691
GTGTAGG706.950697E-613.6254741
AATCCCG852.7315764E-713.39418419
GTCTTAT801.9056843E-613.114521
CTCCTAG751.4154011E-512.7171111
TCTTATA603.9559125E-412.7147872
TAGGGTG604.0019045E-412.6973855
TAGGACC2300.012.4213554
GTCTAGG701.0487661E-412.2629261
TGTAGGA5150.012.2210082
TGGCGAG3350.012.17804418
TAGCACC550.003009003612.1202314
GGCGAGA2350.012.11176219
GCCAATG550.003096470412.07396213
GGTATCA5400.012.0106031
GTAGGAC4600.012.0105973
TAGGACA1750.011.971824
CAGGGGT802.7884484E-511.9037984
Produced by FastQC (version 0.11.5)