FastQCFastQC Report
Thu 26 May 2016
SRR1926984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714653
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16000.22388487839552904No Hit
CTGTAGGACGTGGAATATGGCAAGA12770.1786881185694316No Hit
GTATCAACGCAGAGTACTTTTTTTT12260.1715517880705741No Hit
GTCCTAAAGTGTGTATTTCTCATTT12080.1690330831886244No Hit
GTCCTACAGTGTGCATTTCTCATTT11310.15825862341583957No Hit
CTTTAGGACGTGAAATATGGCGAGG9920.138808624605228No Hit
GTACATGGGAAGCAGTGGTATCAAC8440.11809927335364155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCG400.0053392714.22417516
GGAGTTT400.0053392714.22417513
TAGGACC3050.012.8002014
GCTATTC550.00298273412.1352413
TTAACCC550.003012742412.1190394
CTGTGCG550.00311084212.0673099
GTCCTAG1209.309588E-911.9302941
ATGTCGA802.9148867E-511.85347917
TGTAGGA7850.011.7818952
TCGAACT658.136982E-411.67111919
TAAACTG907.294291E-611.6324015
GTCTTAA751.9833332E-411.4530821
GATATAC1604.1836756E-1111.3337781
AGGACGT6600.011.2478595
AGGACCT4700.011.1374055
GGACCTG4450.011.1214796
GGACGTG6350.011.09116656
GTAGGAC7750.011.0727083
TTAATAC951.3032497E-511.0403323
CTGTAGG8300.011.0391151
Produced by FastQC (version 0.11.5)