##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714653 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.179536082546356 31.0 28.0 31.0 16.0 34.0 2 28.223715565456242 31.0 28.0 31.0 16.0 34.0 3 28.005268291044743 31.0 28.0 31.0 16.0 34.0 4 31.521488050844255 35.0 32.0 37.0 19.0 37.0 5 31.80423646161144 35.0 32.0 37.0 19.0 37.0 6 30.94845750315188 35.0 32.0 37.0 11.0 37.0 7 31.000908133037992 35.0 32.0 37.0 15.0 37.0 8 30.82531942075385 35.0 32.0 37.0 11.0 37.0 9 33.015271747267555 37.0 33.0 39.0 17.0 39.0 10 33.190471459575484 37.0 33.0 39.0 17.0 39.0 11 33.36551725102952 38.0 34.0 39.0 17.0 39.0 12 33.243807834011754 38.0 33.0 39.0 17.0 39.0 13 33.34620158314595 38.0 34.0 39.0 17.0 39.0 14 34.358720945689726 39.0 34.0 41.0 17.0 41.0 15 33.92967636041548 39.0 33.0 41.0 11.0 41.0 16 34.01803672551574 39.0 33.0 41.0 16.0 41.0 17 34.23811136313707 39.0 34.0 41.0 17.0 41.0 18 34.28627039976044 39.0 34.0 41.0 17.0 41.0 19 33.66757013543636 39.0 33.0 41.0 10.0 41.0 20 33.95292260719538 39.0 34.0 41.0 10.0 41.0 21 33.943057679741074 39.0 34.0 41.0 10.0 41.0 22 33.95586249550481 39.0 34.0 41.0 8.0 41.0 23 33.939241841844925 39.0 34.0 41.0 2.0 41.0 24 32.665265520469376 39.0 32.0 40.0 2.0 41.0 25 33.237215823623494 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.11777305956179873 1101 2 -0.23904348745356074 1101 3 -1.0694823928356598 1101 4 -0.5174318276768552 1101 5 -0.1286951649709458 1101 6 -2.398635236735519 1101 7 -0.7096067095578675 1101 8 -0.05595921574558105 1101 9 -0.12489086781423708 1101 10 -8.466293315128311 1101 11 -3.9347396228215565 1101 12 -1.6683276953086512 1101 13 -1.0101788708194803 1101 14 -0.6753488370138498 1101 15 -2.2981255416628095 1101 16 -0.29236202894810503 1101 17 -0.2534370278620699 1101 18 -0.06509806232874382 1101 19 -0.6667377028647721 1101 20 -0.056903242005517995 1101 21 -0.06377067331273878 1101 22 0.2169875749229746 1101 23 -0.14300716222901855 1101 24 -0.11890366669484109 1101 25 -0.1545809924279098 1102 1 -0.33642317031696223 1102 2 -0.38401008440184015 1102 3 -0.5747306873826226 1102 4 -0.5334944153764134 1102 5 -0.36668109774123536 1102 6 -1.0420565564428799 1102 7 -0.37374233389046907 1102 8 0.2433047698518358 1102 9 0.09804383517658977 1102 10 0.001495905878989845 1102 11 -0.03392771244939752 1102 12 8.594235567898068E-4 1102 13 -0.18666788796667788 1102 14 -0.5127108343133671 1102 15 -0.02103200429287 1102 16 0.03396592989157199 1102 17 -0.30773971157885427 1102 18 -0.3647018147666756 1102 19 -0.05587222405828385 1102 20 -0.15248539056747035 1102 21 -0.22664254150085839 1102 22 -0.4515906111633541 1102 23 -0.7891854471566049 1102 24 -4.047080486653716 1102 25 -2.2504199517706134 1103 1 -0.2761432360804079 1103 2 -0.4479254711137557 1103 3 -0.10321733687576895 1103 4 -0.3857930587855627 1103 5 -0.48784848680938353 1103 6 0.24826091922552607 1103 7 0.0981842361238634 1103 8 0.08402623352808192 1103 9 -0.014640245322084411 1103 10 0.17897632191190382 1103 11 0.019290564161494217 1103 12 -0.01690198174121349 1103 13 -0.10086329683419137 1103 14 -0.10046722822585252 1103 15 0.21482753624220408 1103 16 0.3866496587140702 1103 17 -0.08414048174968514 1103 18 -0.27294070839342766 1103 19 -1.940619149089713 1103 20 -0.622035508818584 1103 21 -0.5498813032490375 1103 22 -0.4272880289810246 1103 23 -0.28357357456888366 1103 24 1.209190339169865 1103 25 0.4742404609213793 1104 1 0.09023625154785009 1104 2 0.17754609903654384 1104 3 -0.9247763296343052 1104 4 -0.40047550644357344 1104 5 -0.031132304000745847 1104 6 -1.097609697064481 1104 7 0.22874408210605424 1104 8 0.5209878822187122 1104 9 0.6739795013361203 1104 10 0.6831824957507564 1104 11 0.4843639632732675 1104 12 0.722241731750664 1104 13 0.5539232878646629 1104 14 0.3389735821178448 1104 15 0.7884572371763738 1104 16 0.332900944018931 1104 17 0.38363172941222956 1104 18 0.4002536297019148 1104 19 -0.8095692464447168 1104 20 0.11338289893481601 1104 21 0.4227945373950348 1104 22 0.41097839557431115 1104 23 0.4098408514741223 1104 24 1.823473740688165 1104 25 1.0954179095638779 1105 1 -0.011424026158941558 1105 2 0.036283640564782615 1105 3 0.4746137376627395 1105 4 -0.0069564673431372626 1105 5 0.2468223769771889 1105 6 -0.5738938779998257 1105 7 0.06889571342047773 1105 8 0.4464559800123915 1105 9 -0.6105864753228758 1105 10 -0.07471511051894453 1105 11 -0.21898299984113834 1105 12 -0.1792336920503459 1105 13 -0.22678120144447433 1105 14 -0.5919532429950252 1105 15 0.07867872111879137 1105 16 -0.6206470583662806 1105 17 -0.49456488017972333 1105 18 -0.5300504847882479 1105 19 -1.051464637874993 1105 20 -0.41662732340098785 1105 21 -0.11213944335289483 1105 22 -0.23185489516595226 1105 23 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2215 2 -0.23868684349660896 2215 3 0.23487771610853514 2215 4 0.5936701787380549 2215 5 0.586226911836274 2215 6 1.28509259007755 2215 7 0.7219941301552559 2215 8 -0.5854045836663531 2215 9 -0.5104986843259951 2215 10 -0.6648128915258127 2215 11 -0.5202256965383825 2215 12 -0.8688864635212994 2215 13 -0.4240278941457163 2215 14 -0.3063464255700268 2215 15 -0.4337376503884869 2215 16 -0.3520308261136833 2215 17 -0.9298220472550724 2215 18 -1.4604382788124042 2215 19 -0.40403782226636054 2215 20 -4.442869263279292 2215 21 -2.6555218185534564 2215 22 -1.8091715179371235 2215 23 -1.5431593407226813 2215 24 -1.7779216081490432 2215 25 -1.3085029053505934 2216 1 -0.533834865733958 2216 2 -1.1681702803070486 2216 3 -0.3977278510967608 2216 4 -0.34952020542926476 2216 5 -0.5641547884775839 2216 6 -1.5589502809666769 2216 7 -10.035016957656374 2216 8 -10.925306999959542 2216 9 -5.947263894089847 2216 10 -3.36026538114384 2216 11 -2.5338874154918223 2216 12 -2.1767370179118792 2216 13 -1.8778072069480913 2216 14 -2.0929327775566477 2216 15 -2.964261340477986 2216 16 -2.010428918297393 2216 17 -2.291072269970698 2216 18 -1.9435598609841875 2216 19 -1.3300852709732425 2216 20 -2.324965668306497 2216 21 -5.53747157390891 2216 22 -3.5672475290660515 2216 23 -3.631201224965384 2216 24 -3.85122177398652 2216 25 -3.272891705153235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42437.0 3 6.0 4 318.0 5 1887.0 6 2506.0 7 2114.0 8 1679.0 9 1519.0 10 1468.0 11 1270.0 12 1383.0 13 1299.0 14 1261.0 15 1369.0 16 1510.0 17 1541.0 18 1810.0 19 2256.0 20 2929.0 21 3823.0 22 4764.0 23 5597.0 24 6610.0 25 7502.0 26 8648.0 27 9142.0 28 10502.0 29 12220.0 30 14758.0 31 17906.0 32 23322.0 33 30554.0 34 41070.0 35 57150.0 36 82895.0 37 132544.0 38 175084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23849700995187 19.277115192586027 13.050325932075978 25.434061865386127 2 13.749395733352015 20.605737905909553 42.78207867726906 22.862787683469364 3 18.607091912372592 25.13346729105538 29.921334869082948 26.338105927489075 4 11.9246258457678 17.252822960075278 39.12726284894923 31.69528834520769 5 12.354392609169679 38.23783391905188 36.774867146158115 12.632906325620322 6 30.376719182769758 37.52706396402575 19.720620116415283 12.375596736789216 7 27.05721049876575 31.480299026493903 23.107192325342172 18.355298149398173 8 23.83798187356962 37.63640777312074 22.1011111875071 16.424499165802533 9 25.899122500104966 15.975536366562634 21.040824038178943 37.08451709515346 10 14.282774780726925 29.62755072045867 35.217229418565914 20.872445080248486 11 33.05185199698273 23.328570448938006 25.359490478662885 18.26008707541638 12 22.93158004486784 25.461308836557073 32.339831192804354 19.267279925770737 13 28.235335278599965 23.00004898153396 26.01885089321326 22.74576484665281 14 19.94018546145643 23.511983900176617 28.152858754278963 28.394971884087987 15 22.397124927927273 32.237921170629036 23.590453372443868 21.774500528999827 16 20.83700126097404 29.05852403058247 29.266353825677687 20.8381208827658 17 20.777563187505248 29.78419682592997 28.64052397346545 20.797716013099336 18 20.551223417058985 28.401552852400652 30.829913960557327 20.21730976998304 19 23.075006718022216 27.567544115012538 28.08875067180222 21.268698495163026 20 22.67470908060374 28.249165552904298 29.060451056266572 20.015674310225386 21 22.087685710767026 27.649212373627112 27.76984740088895 22.493254514716913 22 22.005052166063717 29.353784576198837 27.867244190358896 20.77391906737854 23 21.06085182194394 28.909128822919396 28.64000425449136 21.39001510064531 24 22.51107696355448 27.74279052713191 29.10953248636197 20.636600022951637 25 21.671200976276044 28.18829140496423 28.875015394261023 21.265492224498708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 269.0 1 269.0 2 347.5 3 426.0 4 426.0 5 426.0 6 622.5 7 819.0 8 819.0 9 819.0 10 1226.5 11 1634.0 12 1634.0 13 1634.0 14 2782.5 15 3931.0 16 3931.0 17 3931.0 18 6834.5 19 9738.0 20 9738.0 21 9738.0 22 15281.0 23 20824.0 24 20824.0 25 20824.0 26 29536.0 27 38248.0 28 38248.0 29 38248.0 30 49876.5 31 61505.0 32 61505.0 33 61505.0 34 69547.5 35 77590.0 36 77590.0 37 77590.0 38 82556.0 39 87522.0 40 87522.0 41 87522.0 42 89988.0 43 92454.0 44 92454.0 45 92454.0 46 93415.5 47 94377.0 48 94377.0 49 94377.0 50 88142.5 51 81908.0 52 81908.0 53 81908.0 54 70498.5 55 59089.0 56 59089.0 57 59089.0 58 49302.5 59 39516.0 60 39516.0 61 39516.0 62 31553.5 63 23591.0 64 23591.0 65 23591.0 66 17940.0 67 12289.0 68 12289.0 69 12289.0 70 8915.0 71 5541.0 72 5541.0 73 5541.0 74 3868.5 75 2196.0 76 2196.0 77 2196.0 78 1497.5 79 799.0 80 799.0 81 799.0 82 546.0 83 293.0 84 293.0 85 293.0 86 183.0 87 73.0 88 73.0 89 73.0 90 44.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22682336742447032 2 0.1368496319192671 3 0.1392284087522196 4 0.07122337693957767 5 0.020429495153592023 6 0.0198697829576032 7 0.2902107736202045 8 0.27873667360243365 9 0.017211150026656293 10 0.03456222810230979 11 0.013992804899720565 12 0.01539208538969262 13 0.013712948801726153 14 0.015532013438689825 15 0.013573020752728947 16 0.01763093417364791 17 0.015811869536684238 18 0.013573020752728947 19 0.021968703692561285 20 0.01511222929169821 21 0.014132732948717771 22 0.01511222929169821 23 0.015951797585681445 24 0.014972301242701004 25 0.014132732948717771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714653.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.12971325045734 #Duplication Level Percentage of deduplicated Percentage of total 1 93.19571315418122 79.33724336987322 2 5.1967782318548785 8.84800481408049 3 0.814791497793127 2.080888996981186 4 0.2600012406839437 0.8853532425674906 5 0.14681421367644434 0.624912595568354 6 0.08460419092730587 0.4321398308057095 7 0.05839201867291431 0.3479627064018382 8 0.03727762558358212 0.25387468612706066 9 0.026168303993370393 0.20049301936857772 >10 0.14887299613924812 2.3429213496539694 >50 0.016280271126432315 0.9898833956961929 >100 0.012497418491519745 2.1233237130953855 >500 9.866382959674693E-4 0.6312016476604932 >1k 8.221985799728911E-4 0.9017966321199598 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1600 0.22388487839552904 No Hit CTGTAGGACGTGGAATATGGCAAGA 1277 0.1786881185694316 No Hit GTATCAACGCAGAGTACTTTTTTTT 1226 0.1715517880705741 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1208 0.1690330831886244 No Hit GTCCTACAGTGTGCATTTCTCATTT 1131 0.15825862341583957 No Hit CTTTAGGACGTGAAATATGGCGAGG 992 0.138808624605228 No Hit GTACATGGGAAGCAGTGGTATCAAC 844 0.11809927335364155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.3992804899720565E-4 0.0 8 0.0 0.0 0.0 1.3992804899720565E-4 0.0 9 0.0 0.0 0.0 1.3992804899720565E-4 0.0 10 0.0 0.0 0.0 1.3992804899720565E-4 0.0 11 0.0 1.3992804899720565E-4 0.0 1.3992804899720565E-4 0.0 12 0.0 1.3992804899720565E-4 0.0 1.3992804899720565E-4 0.0 13 0.0 1.3992804899720565E-4 0.0 1.3992804899720565E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGGCG 40 0.00533927 14.224175 16 GGAGTTT 40 0.00533927 14.224175 13 TAGGACC 305 0.0 12.800201 4 GCTATTC 55 0.002982734 12.135241 3 TTAACCC 55 0.0030127424 12.119039 4 CTGTGCG 55 0.003110842 12.067309 9 GTCCTAG 120 9.309588E-9 11.930294 1 ATGTCGA 80 2.9148867E-5 11.853479 17 TGTAGGA 785 0.0 11.781895 2 TCGAACT 65 8.136982E-4 11.671119 19 TAAACTG 90 7.294291E-6 11.632401 5 GTCTTAA 75 1.9833332E-4 11.453082 1 GATATAC 160 4.1836756E-11 11.333778 1 AGGACGT 660 0.0 11.247859 5 AGGACCT 470 0.0 11.137405 5 GGACCTG 445 0.0 11.121479 6 GGACGTG 635 0.0 11.0911665 6 GTAGGAC 775 0.0 11.072708 3 TTAATAC 95 1.3032497E-5 11.040332 3 CTGTAGG 830 0.0 11.039115 1 >>END_MODULE