FastQCFastQC Report
Thu 26 May 2016
SRR1926984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714653
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19040.2664230052906795No Hit
GTCCTACAGTGGACATTTCTAAATT16120.2255640149834955No Hit
CTGTAGGACGTGGAATATGGCAAGA15680.21940718082761845No Hit
GTCCTAAAGTGTGTATTTCTCATTT12930.1809269673533869No Hit
CTTTAGGACGTGAAATATGGCGAGG12000.16791365879664677No Hit
GTCCTACAGTGTGCATTTCTCATTT11670.16329603317973898No Hit
GGTATCAACGCAGAGTACTTTTTTT11500.1609172563467865No Hit
TATCAACGCAGAGTACTTTTTTTTT9750.1364298477722755No Hit
GTACATGGGAAGCAGTGGTATCAAC9320.13041294166539566No Hit
CCCATGTACTCTGCGTTGATACCAC8610.12047805018659405No Hit
CTGTAGGACCTGGAATATGGCGAGA8520.11921869774561919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCC350.002130204816.3293765
TAGGACC3400.016.2493294
CTAACCA551.0983682E-515.58713154
TCCAACG400.005321786314.23121118
TACACGG551.9138392E-413.8552285
TGTAGGA8750.013.5141432
GGACCTG5250.012.8820636
CTGTAGG9250.012.6832291
AGGACCT5600.012.5872285
GTAGGAC8950.012.5604843
CTAGGAC1155.0840754E-912.42627053
GATATAC1404.9112714E-1112.2634021
CTAAACT701.06240994E-412.2470324
GGCGAGG3100.012.24190119
TAGTCCC550.003004773512.1233244
TCGAACT550.003096097812.07496619
AATGCCG802.900177E-511.85934318
GACGTGG3600.011.8576827
GTACAAG657.7486853E-411.7393261
CCTAATA657.7624223E-411.7368492
Produced by FastQC (version 0.11.5)