##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714653 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.864665788851372 31.0 30.0 33.0 26.0 34.0 2 30.168652478895353 31.0 30.0 34.0 26.0 34.0 3 30.23082810818677 31.0 30.0 34.0 26.0 34.0 4 33.81384951857755 35.0 35.0 37.0 28.0 37.0 5 33.79405949460787 37.0 35.0 37.0 30.0 37.0 6 33.74504129976366 37.0 35.0 37.0 29.0 37.0 7 33.73568571040771 37.0 35.0 37.0 29.0 37.0 8 33.75966378088387 37.0 35.0 37.0 29.0 37.0 9 35.28941458302141 39.0 35.0 39.0 29.0 39.0 10 35.175135345405394 39.0 35.0 39.0 29.0 39.0 11 35.212585688439006 39.0 35.0 39.0 29.0 39.0 12 35.123464114752196 39.0 35.0 39.0 29.0 39.0 13 35.13860712821467 39.0 35.0 39.0 29.0 39.0 14 36.237627212087546 40.0 36.0 41.0 29.0 41.0 15 36.22323981008965 40.0 36.0 41.0 29.0 41.0 16 36.20131868193375 40.0 36.0 41.0 29.0 41.0 17 36.168217302662974 40.0 36.0 41.0 29.0 41.0 18 36.14934660597521 40.0 36.0 41.0 29.0 41.0 19 36.22402200788355 40.0 37.0 41.0 29.0 41.0 20 36.191687434321274 40.0 37.0 41.0 29.0 41.0 21 36.13749190166416 40.0 37.0 41.0 29.0 41.0 22 36.062753532133776 40.0 37.0 41.0 28.0 41.0 23 35.98441061606122 40.0 37.0 41.0 28.0 41.0 24 35.922055878867084 40.0 36.0 41.0 27.0 41.0 25 35.80017295106856 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09107501849817012 1101 2 -0.13497813988841756 1101 3 0.21370913687195525 1101 4 0.005717384177906126 1101 5 -0.11186662551465787 1101 6 -0.18957324347356774 1101 7 0.015448076209040096 1101 8 0.07442943913518008 1101 9 -0.009066552173251807 1101 10 -0.03791052993695132 1101 11 -0.09213434274848709 1101 12 0.07761459900786605 1101 13 -0.0013323825241045029 1101 14 0.18603259294519603 1101 15 0.024938934387535028 1101 16 0.2532591852508048 1101 17 0.16090425809804998 1101 18 0.2089440394215174 1101 19 0.10120780494713699 1101 20 0.29555992660255725 1101 21 -0.0030522014898437533 1101 22 -0.005157207177447276 1101 23 0.0037650450314501427 1101 24 0.03300943238962617 1101 25 0.12639730019879636 1102 1 -0.37759818883167284 1102 2 -0.19048721651687117 1102 3 -0.0787718670728701 1102 4 -0.014868073801324044 1102 5 -0.039640137821280064 1102 6 -0.0681979379040385 1102 7 0.06274450292088574 1102 8 -0.032244111119425156 1102 9 0.04775658374231284 1102 10 -0.00506875538328444 1102 11 -0.12271777095178038 1102 12 0.11628977308321709 1102 13 0.1260863602503406 1102 14 0.12256238211325154 1102 15 0.15753095825063923 1102 16 0.10325433143879792 1102 17 0.12463127971074783 1102 18 0.031170968370531682 1102 19 -0.13995163836474944 1102 20 -0.04341441879470409 1102 21 -0.15440994508502115 1102 22 -0.04905067041643463 1102 23 -0.12262645461995447 1102 24 -0.11729282772174798 1102 25 -0.07683622110943844 1103 1 -0.5342734422583177 1103 2 -0.4051037576058363 1103 3 -0.18873836991705062 1103 4 -0.2842942262001955 1103 5 -0.1671878119229646 1103 6 -0.3045577378002946 1103 7 -0.3213834720397699 1103 8 -0.21468052367074364 1103 9 -0.09388627151656692 1103 10 -0.022554094882671905 1103 11 -0.2714636057870621 1103 12 -0.2628857221207994 1103 13 -0.5098971461369786 1103 14 -0.2888372011504785 1103 15 -0.2846912835091473 1103 16 -0.15502104859126575 1103 17 -0.27407806872121654 1103 18 -0.2788579569949903 1103 19 -0.41475579869391765 1103 20 -0.41570409446135415 1103 21 -0.5098349897039327 1103 22 -0.24161184009140158 1103 23 -0.3650843194180524 1103 24 -0.4194231061613607 1103 25 -0.5498055109873192 1104 1 -0.19477706298946273 1104 2 -0.09458197852513095 1104 3 -0.013459978353026258 1104 4 0.06606215875682153 1104 5 -0.06835563445244475 1104 6 -0.15228400615007587 1104 7 -0.15962889416670123 1104 8 -0.25886875793533193 1104 9 -0.31758138626420873 1104 10 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0.4221966408233797 2216 25 0.4764952523643089 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17754.0 3 19.0 4 305.0 5 1927.0 6 2515.0 7 1892.0 8 1463.0 9 1286.0 10 1120.0 11 1020.0 12 953.0 13 918.0 14 891.0 15 853.0 16 890.0 17 986.0 18 1049.0 19 1287.0 20 1623.0 21 2207.0 22 2759.0 23 3306.0 24 4053.0 25 4742.0 26 5833.0 27 6270.0 28 7224.0 29 8728.0 30 11189.0 31 14388.0 32 18455.0 33 24861.0 34 33866.0 35 47005.0 36 69655.0 37 121076.0 38 290285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.855078270566516 20.097477465866078 11.026385320381168 28.021058943186244 2 13.638426689901914 21.19188970355449 40.946098523796046 24.223585082747547 3 18.20739111084987 25.4826222405092 29.42225282491806 26.887733823722876 4 11.9278866806688 17.984112569316856 38.47643541690863 31.611565333105716 5 12.618193674600587 39.086440035207964 35.40317739301522 12.892188897176226 6 30.853216536046745 38.5096830998277 18.10854115264767 12.528559211477889 7 27.578861627042834 32.335541370251825 22.062921354604757 18.022675648100588 8 24.422280593973777 38.543222074038205 20.137367981337214 16.897129350650804 9 25.981804620075934 16.19587532204367 20.601978513162333 37.22034154471807 10 14.627634267162968 30.24908342895525 34.11351487503848 21.0097674288433 11 33.109576485240304 23.440174079749234 24.90977659299063 18.540472842019827 12 23.22980028013674 25.612081981274777 31.72233719675758 19.43578054183091 13 28.456806707637888 23.04253282734997 25.845031400073882 22.655629064938264 14 20.129769272640008 23.70605034891059 27.780335351562226 28.383845026887172 15 22.182326510793533 32.3733175118696 23.496552814630004 21.94780316270687 16 20.944938081578396 29.262995872105225 28.869516546561254 20.922549499755124 17 20.572530406407594 30.051829421872462 28.35575916669932 21.019881005020626 18 20.432099629189114 28.519135241027076 30.789757223815855 20.25900790596796 19 22.6473864202437 27.69207950163156 28.238779154049805 21.421754924074936 20 22.82497512758047 28.299146291805606 28.71501594493505 20.16086263567887 21 21.905123095352717 27.575402201050146 27.805471277751032 22.714003425846105 22 22.227601685855493 29.320144629833823 27.595529012722274 20.85672467158841 23 20.941940972669215 29.047004695991895 28.545082081908397 21.465972249430493 24 22.23515781107448 27.770859103451752 29.212539809585646 20.781443275888126 25 21.6356642192155 28.427376189616595 28.714661337967957 21.22229825319995 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 292.0 1 292.0 2 310.0 3 328.0 4 328.0 5 328.0 6 523.5 7 719.0 8 719.0 9 719.0 10 1161.0 11 1603.0 12 1603.0 13 1603.0 14 2692.0 15 3781.0 16 3781.0 17 3781.0 18 6559.0 19 9337.0 20 9337.0 21 9337.0 22 14621.0 23 19905.0 24 19905.0 25 19905.0 26 28197.0 27 36489.0 28 36489.0 29 36489.0 30 48210.0 31 59931.0 32 59931.0 33 59931.0 34 67826.0 35 75721.0 36 75721.0 37 75721.0 38 80804.0 39 85887.0 40 85887.0 41 85887.0 42 89237.5 43 92588.0 44 92588.0 45 92588.0 46 94338.5 47 96089.0 48 96089.0 49 96089.0 50 90105.5 51 84122.0 52 84122.0 53 84122.0 54 72445.0 55 60768.0 56 60768.0 57 60768.0 58 50718.5 59 40669.0 60 40669.0 61 40669.0 62 32518.0 63 24367.0 64 24367.0 65 24367.0 66 18617.0 67 12867.0 68 12867.0 69 12867.0 70 9281.5 71 5696.0 72 5696.0 73 5696.0 74 3989.0 75 2282.0 76 2282.0 77 2282.0 78 1575.5 79 869.0 80 869.0 81 869.0 82 556.5 83 244.0 84 244.0 85 244.0 86 161.5 87 79.0 88 79.0 89 79.0 90 48.0 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11851905750063316 2 0.11124279895277848 3 0.01665143783066747 4 0.0 5 0.005876978057882637 6 0.3548575322569135 7 0.0198697829576032 8 0.010634531723787628 9 0.010634531723787628 10 0.004617625616907786 11 0.005317265861893814 12 2.798560979944113E-4 13 6.996402449860282E-4 14 0.0 15 0.0016791365879664675 16 4.197841469916169E-4 17 1.3992804899720565E-4 18 4.197841469916169E-4 19 1.3992804899720565E-4 20 8.395682939832338E-4 21 0.01189388416476248 22 1.3992804899720565E-4 23 1.3992804899720565E-4 24 1.3992804899720565E-4 25 0.004477697567910581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714653.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.26016316901146 #Duplication Level Percentage of deduplicated Percentage of total 1 91.47209342874059 75.24509330859263 2 6.553608495806642 10.782018084217482 3 1.033667623671458 2.550890021572154 4 0.32586750905330114 1.0722365786481547 5 0.16066433598330745 0.6608137246713877 6 0.09789975202897656 0.48319497456656235 7 0.06428775384861023 0.3701824784949146 8 0.05253200233045405 0.3457032866638433 9 0.03167848517117275 0.23452896232150022 >10 0.1731429654435551 2.628093577677725 >50 0.01702379863515268 0.9981824665471181 >100 0.015320839190590711 2.5614790207552045 >500 0.0010213892751918126 0.6820995125558238 >1k 0.0011916208210571147 1.3854840027155246 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1904 0.2664230052906795 No Hit GTCCTACAGTGGACATTTCTAAATT 1612 0.2255640149834955 No Hit CTGTAGGACGTGGAATATGGCAAGA 1568 0.21940718082761845 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1293 0.1809269673533869 No Hit CTTTAGGACGTGAAATATGGCGAGG 1200 0.16791365879664677 No Hit GTCCTACAGTGTGCATTTCTCATTT 1167 0.16329603317973898 No Hit GGTATCAACGCAGAGTACTTTTTTT 1150 0.1609172563467865 No Hit TATCAACGCAGAGTACTTTTTTTTT 975 0.1364298477722755 No Hit GTACATGGGAAGCAGTGGTATCAAC 932 0.13041294166539566 No Hit CCCATGTACTCTGCGTTGATACCAC 861 0.12047805018659405 No Hit CTGTAGGACCTGGAATATGGCGAGA 852 0.11921869774561919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.3992804899720565E-4 0.0 9 0.0 0.0 0.0 1.3992804899720565E-4 0.0 10 0.0 0.0 0.0 1.3992804899720565E-4 0.0 11 0.0 0.0 0.0 2.798560979944113E-4 0.0 12 0.0 0.0 0.0 2.798560979944113E-4 0.0 13 0.0 1.3992804899720565E-4 0.0 2.798560979944113E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCC 35 0.0021302048 16.329376 5 TAGGACC 340 0.0 16.249329 4 CTAACCA 55 1.0983682E-5 15.5871315 4 TCCAACG 40 0.0053217863 14.231211 18 TACACGG 55 1.9138392E-4 13.855228 5 TGTAGGA 875 0.0 13.514143 2 GGACCTG 525 0.0 12.882063 6 CTGTAGG 925 0.0 12.683229 1 AGGACCT 560 0.0 12.587228 5 GTAGGAC 895 0.0 12.560484 3 CTAGGAC 115 5.0840754E-9 12.4262705 3 GATATAC 140 4.9112714E-11 12.263402 1 CTAAACT 70 1.06240994E-4 12.247032 4 GGCGAGG 310 0.0 12.241901 19 TAGTCCC 55 0.0030047735 12.123324 4 TCGAACT 55 0.0030960978 12.074966 19 AATGCCG 80 2.900177E-5 11.859343 18 GACGTGG 360 0.0 11.857682 7 GTACAAG 65 7.7486853E-4 11.739326 1 CCTAATA 65 7.7624223E-4 11.736849 2 >>END_MODULE