FastQCFastQC Report
Thu 26 May 2016
SRR1926983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713587
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18300.25645086023147845No Hit
CTGTAGGACGTGGAATATGGCAAGA15110.21174713104358686No Hit
GTCCTACAGTGTGCATTTCTCATTT14280.2001157532298094No Hit
GTCCTAAAGTGTGTATTTCTCATTT13780.1931088991251242No Hit
CTTTAGGACGTGAAATATGGCGAGG10430.14616297662373334No Hit
GTATCAACGCAGAGTACTTTTTTTT10430.14616297662373334No Hit
CTGTAGGACCTGGAATATGGCGAGA8320.11659405230196176No Hit
GTACATGGGAAGCAGTGGTATCAAC7390.1035613036672473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG753.3578544E-916.4348418
GCCGGTT508.853504E-515.16955811
CCTATCC456.587217E-414.8271493
GGCGTGC456.6899776E-414.7969178
CCAACGA653.4341974E-614.58713719
TAGTACG400.00518650414.2865374
CGGTTTC400.00534353914.22245813
TAGGACC4450.013.6979524
GTATAGG603.9501922E-412.7179451
TGCCGGT604.1421843E-412.64661610
AGGACCT6400.012.6433085
CCGGTTT604.1569068E-412.64129912
GGCGAGG2950.012.21363619
TGTGCGT550.003102232712.07177110
ACTGTGC1353.619789E-1011.9784578
GTGTTAC802.7453196E-511.9230741
GTAGGAC9150.011.8756093
AATGTCC1459.458745E-1111.8083778
CTGTAGG9700.011.8001561
TGCAAGG657.792859E-411.731372
Produced by FastQC (version 0.11.5)