##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713587 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.141410928169936 31.0 28.0 31.0 16.0 34.0 2 28.240716268653998 31.0 28.0 31.0 16.0 34.0 3 28.053455289964642 31.0 28.0 33.0 16.0 34.0 4 31.50584721975036 35.0 32.0 37.0 17.0 37.0 5 31.760617836367533 35.0 32.0 37.0 19.0 37.0 6 30.96116240906855 35.0 32.0 37.0 11.0 37.0 7 30.982729506002773 35.0 32.0 37.0 11.0 37.0 8 30.81061874725857 35.0 32.0 37.0 11.0 37.0 9 32.96204947679821 37.0 33.0 39.0 17.0 39.0 10 33.12832072333156 37.0 33.0 39.0 17.0 39.0 11 33.2859763420578 38.0 34.0 39.0 17.0 39.0 12 33.165384178803706 38.0 33.0 39.0 17.0 39.0 13 33.264365802628134 38.0 34.0 39.0 17.0 39.0 14 34.271973844815 39.0 34.0 41.0 17.0 41.0 15 33.85495812003302 39.0 33.0 41.0 10.0 41.0 16 33.940458556560024 39.0 33.0 41.0 11.0 41.0 17 34.17318981427633 39.0 34.0 41.0 17.0 41.0 18 34.22760784599495 39.0 34.0 41.0 15.0 41.0 19 33.60600879780601 39.0 33.0 41.0 10.0 41.0 20 33.90122858179871 39.0 34.0 41.0 10.0 41.0 21 33.88562151496594 39.0 34.0 41.0 8.0 41.0 22 33.90805045495504 39.0 34.0 41.0 2.0 41.0 23 33.898777584232896 39.0 34.0 41.0 2.0 41.0 24 32.61172498938462 39.0 32.0 41.0 2.0 41.0 25 33.18751742954959 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.37136761823025566 1101 2 -0.395433978669093 1101 3 -1.150964600423908 1101 4 -0.8599153895796618 1101 5 -0.5473814698959387 1101 6 -2.771789578401595 1101 7 -1.177404115080524 1101 8 -0.3390514589711877 1101 9 -0.8192358522136374 1101 10 -10.112749107021564 1101 11 -4.643960954935981 1101 12 -1.9405386933678663 1101 13 -1.2557289053444265 1101 14 -1.2409215350995524 1101 15 -2.669720935458848 1101 16 -0.9468578899196416 1101 17 -0.664217372113086 1101 18 -0.6740617310513244 1101 19 -1.1898387497711553 1101 20 -0.6447751325813513 1101 21 -0.6386932748525638 1101 22 -0.3816212607227243 1101 23 -0.5167842172432131 1101 24 -0.6353507170142869 1101 25 -0.514392465553378 1102 1 0.13866746037179922 1102 2 0.03366653668321007 1102 3 -0.002079753513857696 1102 4 -0.1870376450038549 1102 5 0.16510737857316116 1102 6 -0.8451653525127192 1102 7 0.18274226229594603 1102 8 0.5179837495516111 1102 9 0.5300413553564098 1102 10 0.5948746424912343 1102 11 0.3733774019996261 1102 12 0.47028197269250427 1102 13 0.28524983095976353 1102 14 0.11105022879114301 1102 15 0.5566997479242843 1102 16 0.5242997039480954 1102 17 0.19100500890115768 1102 18 0.13461264424640973 1102 19 0.7397840469894845 1102 20 0.5174621027051245 1102 21 0.547738668328762 1102 22 0.34233525913349183 1102 23 -0.2818156147573916 1102 24 -3.154006470338093 1102 25 -1.6478626487416363 1103 1 -0.5302163145609065 1103 2 -0.7656968799023218 1103 3 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1 0.6915376733100196 2215 2 0.10866879894346937 2215 3 0.6440670219318072 2215 4 1.0508021107672931 2215 5 0.9957760074529354 2215 6 1.7686596771347993 2215 7 1.403050691715805 2215 8 -0.05999755227200865 2215 9 0.6435734818334282 2215 10 0.5080395684081651 2215 11 0.46610149673831813 2215 12 -0.048549557976599544 2215 13 0.48928903712072724 2215 14 0.45836909318411756 2215 15 0.41277713151313833 2215 16 0.6564847616000122 2215 17 0.3597672429665124 2215 18 -0.7760185501793799 2215 19 0.48052902119972174 2215 20 -2.815954577058836 2215 21 -1.6202234400025475 2215 22 -0.7122876101745419 2215 23 -0.4399857081530243 2215 24 -0.5317229686449707 2215 25 -0.19299116893766666 2216 1 -0.6000224289917675 2216 2 -1.1317404082176559 2216 3 -0.34919036007237736 2216 4 -0.631326076475986 2216 5 -0.7069287898810153 2216 6 -1.5508784147818133 2216 7 -10.306164219545142 2216 8 -10.364384129037102 2216 9 -5.8458088323570685 2216 10 -3.4990518056908826 2216 11 -2.084429387552156 2216 12 -2.0344830618129173 2216 13 -1.7499576496962916 2216 14 -1.9332840107451972 2216 15 -3.2011498622092525 2216 16 -2.241949711003258 2216 17 -2.2122702676899237 2216 18 -1.839182155542474 2216 19 -1.1956315616104831 2216 20 -2.15494550940015 2216 21 -5.040397043040599 2216 22 -3.531244441919096 2216 23 -3.5860757647290136 2216 24 -3.671264160615827 2216 25 -2.986488805146301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44326.0 3 1.0 4 317.0 5 2043.0 6 2641.0 7 2097.0 8 1632.0 9 1513.0 10 1454.0 11 1364.0 12 1342.0 13 1301.0 14 1356.0 15 1326.0 16 1428.0 17 1658.0 18 1827.0 19 2227.0 20 2876.0 21 3593.0 22 4598.0 23 5676.0 24 6547.0 25 7374.0 26 8335.0 27 9192.0 28 10250.0 29 12033.0 30 14347.0 31 17684.0 32 22381.0 33 29186.0 34 39736.0 35 55497.0 36 81160.0 37 129542.0 38 183727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75513027017387 19.076474132035383 12.669200965598787 25.499194632191962 2 13.439181651710856 20.292848573292826 43.78626404080516 22.48170573419116 3 18.83827349153627 24.838590627100494 29.752227316036816 26.570908565326416 4 11.374351753945572 16.81499078642168 39.766056448242686 32.04460101139006 5 11.893574730656768 38.59254669877573 37.25809571601986 12.255782854547647 6 30.506082124804806 38.01138768884864 19.595683779972582 11.886846406373966 7 27.41893549833254 31.337211181581953 22.81962384216155 18.42422947792395 8 23.569052901659315 38.80274674396744 21.752082048226796 15.87611830614645 9 25.889570724131456 15.876170745997529 20.866401096667666 37.36785743320335 10 13.679939658870735 30.22033484840413 35.79053328435757 20.309192208367566 11 33.61580931049418 23.106918045056926 25.20530434380401 18.07196830064489 12 22.772960009643324 25.44645923243072 32.94133972110052 18.83924103682544 13 28.620925998343278 22.980221653161998 26.088047352538414 22.31080499595631 14 19.34789830218643 23.526064304706587 28.594695891916288 28.5313415011907 15 22.191900631146954 32.87766849857666 23.45573631709002 21.47469455318636 16 20.368501464754775 29.400501801157787 29.918282101958145 20.312714632129293 17 20.394289598912305 29.939517965897384 29.100618837034908 20.565573598155403 18 20.006839897345476 28.83773605004338 31.452174680886554 19.703249371724592 19 22.769222574522782 27.699795067605265 28.217032096730332 21.313950261141624 20 22.428280885789054 28.59695869186466 29.402909552690264 19.57185086965602 21 21.782799924873363 27.857405159630083 27.87520568719848 22.484589228298073 22 21.476638002929448 29.962786199356646 28.307017359432052 20.253558438281857 23 20.873315560339872 29.169575102110613 28.962690730290618 20.994418607258893 24 22.340395709872563 27.928718400116615 29.58363001295112 20.147255877059706 25 21.299340952605213 28.594835826836768 29.303360833354358 20.80246238720366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 300.5 3 383.0 4 383.0 5 383.0 6 538.0 7 693.0 8 693.0 9 693.0 10 1072.0 11 1451.0 12 1451.0 13 1451.0 14 2607.5 15 3764.0 16 3764.0 17 3764.0 18 6516.5 19 9269.0 20 9269.0 21 9269.0 22 15010.0 23 20751.0 24 20751.0 25 20751.0 26 29944.0 27 39137.0 28 39137.0 29 39137.0 30 51521.0 31 63905.0 32 63905.0 33 63905.0 34 72656.0 35 81407.0 36 81407.0 37 81407.0 38 86721.5 39 92036.0 40 92036.0 41 92036.0 42 93378.0 43 94720.0 44 94720.0 45 94720.0 46 94921.5 47 95123.0 48 95123.0 49 95123.0 50 87514.0 51 79905.0 52 79905.0 53 79905.0 54 67723.5 55 55542.0 56 55542.0 57 55542.0 58 45792.5 59 36043.0 60 36043.0 61 36043.0 62 28589.5 63 21136.0 64 21136.0 65 21136.0 66 15857.0 67 10578.0 68 10578.0 69 10578.0 70 7627.5 71 4677.0 72 4677.0 73 4677.0 74 3252.5 75 1828.0 76 1828.0 77 1828.0 78 1260.0 79 692.0 80 692.0 81 692.0 82 462.0 83 232.0 84 232.0 85 232.0 86 151.5 87 71.0 88 71.0 89 71.0 90 44.5 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20880425231961905 2 0.1306077605113322 3 0.13425132464576847 4 0.07132977478569537 5 0.025925360187335253 6 0.025925360187335253 7 0.283637454157657 8 0.26738155263478736 9 0.02144097356033672 10 0.043302358366954556 11 0.018778369000556343 12 0.019619191493118568 13 0.01821782067218153 14 0.019619191493118568 15 0.017376998179619303 16 0.022001521888711538 17 0.021300836478243018 18 0.01765727234380671 19 0.02536481185896044 20 0.020179739821493386 21 0.01751713526171301 22 0.019899465657305975 23 0.02031987690358709 24 0.018918506082650048 25 0.01863823191846264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 713587.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.32555789372866 #Duplication Level Percentage of deduplicated Percentage of total 1 93.72107583127003 79.96803081703564 2 4.799929913860297 8.191133955018536 3 0.7287062316317606 1.8653179726384992 4 0.2558151301968554 0.8731027480681408 5 0.11265569076233958 0.48062048320999995 6 0.08037075480452116 0.4114607695221505 7 0.05339786686833505 0.31893419466130163 8 0.03626039038156891 0.2475150431001408 9 0.026088572216211605 0.20034197810991755 >10 0.15530642501399028 2.5753266009117186 >50 0.016264755351702055 0.9731345161130932 >100 0.011992743586085555 2.135867421681733 >500 0.0011499891072456415 0.6051414087007448 >1k 9.857049490676927E-4 1.1540720912284532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1830 0.25645086023147845 No Hit CTGTAGGACGTGGAATATGGCAAGA 1511 0.21174713104358686 No Hit GTCCTACAGTGTGCATTTCTCATTT 1428 0.2001157532298094 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1378 0.1931088991251242 No Hit CTTTAGGACGTGAAATATGGCGAGG 1043 0.14616297662373334 No Hit GTATCAACGCAGAGTACTTTTTTTT 1043 0.14616297662373334 No Hit CTGTAGGACCTGGAATATGGCGAGA 832 0.11659405230196176 No Hit GTACATGGGAAGCAGTGGTATCAAC 739 0.1035613036672473 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 1.4013708209370407E-4 11 0.0 0.0 0.0 0.0 1.4013708209370407E-4 12 0.0 0.0 0.0 0.0 1.4013708209370407E-4 13 0.0 0.0 0.0 0.0 1.4013708209370407E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 75 3.3578544E-9 16.43484 18 GCCGGTT 50 8.853504E-5 15.169558 11 CCTATCC 45 6.587217E-4 14.827149 3 GGCGTGC 45 6.6899776E-4 14.796917 8 CCAACGA 65 3.4341974E-6 14.587137 19 TAGTACG 40 0.005186504 14.286537 4 CGGTTTC 40 0.005343539 14.222458 13 TAGGACC 445 0.0 13.697952 4 GTATAGG 60 3.9501922E-4 12.717945 1 TGCCGGT 60 4.1421843E-4 12.646616 10 AGGACCT 640 0.0 12.643308 5 CCGGTTT 60 4.1569068E-4 12.641299 12 GGCGAGG 295 0.0 12.213636 19 TGTGCGT 55 0.0031022327 12.071771 10 ACTGTGC 135 3.619789E-10 11.978457 8 GTGTTAC 80 2.7453196E-5 11.923074 1 GTAGGAC 915 0.0 11.875609 3 AATGTCC 145 9.458745E-11 11.808377 8 CTGTAGG 970 0.0 11.800156 1 TGCAAGG 65 7.792859E-4 11.73137 2 >>END_MODULE