FastQCFastQC Report
Thu 26 May 2016
SRR1926983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713587
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19460.2727067617543481No Hit
GTATCAACGCAGAGTACTTTTTTTT18180.254769215246354No Hit
CTGTAGGACGTGGAATATGGCAAGA17890.2507052398656366No Hit
GTCCTACAGTGTGCATTTCTCATTT14170.19857424532677864No Hit
GTCCTAAAGTGTGTATTTCTCATTT12730.17839450550528527No Hit
CTTTAGGACGTGAAATATGGCGAGG12050.1688651839229134No Hit
CTGTAGGACCTGGAATATGGCGAGA10880.15246914531795No Hit
GGTATCAACGCAGAGTACTTTTTTT10070.14111804166835998No Hit
TATCAACGCAGAGTACTTTTTTTTT9620.1348118729741433No Hit
GTACATGGGAAGCAGTGGTATCAAC8520.11939679394383587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC451.6883951E-618.97330313
GCCGGTT601.485716E-615.81108611
TAGGACC3000.013.9715174
TAGACAG903.4997356E-813.7598285
CCGGTTT655.516725E-513.13536412
GGCGAGG4250.012.94648719
CCTAGAC603.9938898E-412.701383
GTTAGGG603.9938898E-412.701383
AATCCCG1352.7284841E-1112.64886919
AGGACCT5200.012.6403145
TAGGACT1301.8735591E-1012.4571224
GTCCTAC9200.012.4375131
GGACCTG5150.012.2081216
CCTAACA550.002986058412.1334352
TAGGCTA550.003003431712.12404255
TCCAACG550.00309810312.07391918
ATAGTAC951.0034055E-612.0328853
TGTAGGA9350.012.0314732
GACGTGA3650.011.9574557
CTGTAGG9500.011.9443771
Produced by FastQC (version 0.11.5)