##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713587 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.752253053937363 31.0 30.0 33.0 25.0 34.0 2 30.072806819630962 31.0 30.0 33.0 26.0 34.0 3 30.128843434647774 31.0 30.0 34.0 26.0 34.0 4 33.72663459395981 35.0 35.0 37.0 28.0 37.0 5 33.72588345919979 37.0 35.0 37.0 28.0 37.0 6 33.68395724697899 37.0 35.0 37.0 29.0 37.0 7 33.662282244491564 37.0 35.0 37.0 28.0 37.0 8 33.68682445167863 37.0 35.0 37.0 29.0 37.0 9 35.20746734455645 39.0 35.0 39.0 29.0 39.0 10 35.09791097651723 39.0 35.0 39.0 29.0 39.0 11 35.14055609196917 39.0 35.0 39.0 29.0 39.0 12 35.03810327262128 39.0 35.0 39.0 28.0 39.0 13 35.050688983964115 39.0 35.0 39.0 29.0 39.0 14 36.1418299380454 40.0 36.0 41.0 28.0 41.0 15 36.13289059357864 40.0 36.0 41.0 28.0 41.0 16 36.118422841223286 40.0 36.0 41.0 28.0 41.0 17 36.08953778586213 40.0 36.0 41.0 28.0 41.0 18 36.06411131368705 40.0 36.0 41.0 28.0 41.0 19 36.134561027597194 40.0 37.0 41.0 28.0 41.0 20 36.09951694747802 40.0 37.0 41.0 28.0 41.0 21 36.04783299023104 40.0 37.0 41.0 27.0 41.0 22 35.96689681846782 40.0 36.0 41.0 27.0 41.0 23 35.893556076554084 40.0 36.0 41.0 27.0 41.0 24 35.82887860905538 40.0 36.0 41.0 27.0 41.0 25 35.70641841849697 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11189658879827391 1101 2 0.0677910896231495 1101 3 0.1675808317751013 1101 4 0.13732048072046865 1101 5 0.0747567357950345 1101 6 0.1434018629652698 1101 7 0.0046251371134644614 1101 8 0.1664011655692974 1101 9 0.2118988173744114 1101 10 0.45928192005617774 1101 11 0.38964789426771773 1101 12 0.4042169030694609 1101 13 0.5686955346472189 1101 14 0.396235445538764 1101 15 0.24345557517914074 1101 16 0.24910485847399855 1101 17 0.4331805920404932 1101 18 0.316496297941093 1101 19 0.25020095197671566 1101 20 0.23011324412353673 1101 21 0.3616162943830332 1101 22 0.16351292897601155 1101 23 0.22660165630706075 1101 24 0.41506183858351875 1101 25 0.4397346914637694 1102 1 -0.46278445576454175 1102 2 -0.5140849659829279 1102 3 -0.1450465988255658 1102 4 -0.5183393001465362 1102 5 -0.3062990868198412 1102 6 -0.294441452612233 1102 7 -0.199654452336965 1102 8 -0.4315573464050644 1102 9 -0.39670713299585714 1102 10 -0.33633888458632555 1102 11 -0.3752908264459833 1102 12 -0.3524129527185096 1102 13 -0.25759782640254514 1102 14 -0.4059255695753521 1102 15 -0.28605548973792594 1102 16 -0.37256938690298824 1102 17 -0.19859196003607593 1102 18 -0.48578944109143407 1102 19 -0.39753279710301825 1102 20 -0.1606961621386418 1102 21 -0.2562893831536712 1102 22 -0.40253877948183003 1102 23 -0.30127500559584064 1102 24 -0.3166041784794089 1102 25 -0.40840700876575653 1103 1 -0.5837370400189954 1103 2 -0.5304099983934947 1103 3 -0.402338759026609 1103 4 -0.5554634981260591 1103 5 -0.7849896826362297 1103 6 -0.5578911862989102 1103 7 -0.481668020518903 1103 8 -0.4854300401352063 1103 9 -0.41930862101826705 1103 10 -0.5360521790790429 1103 11 -0.2991576715226145 1103 12 -0.4058864801854867 1103 13 -0.2653008675288149 1103 14 -0.4388455055004883 1103 15 -0.4288554848785111 1103 16 -0.5694937240700568 1103 17 -0.4438440508863195 1103 18 -0.4200458897959649 1103 19 -0.6112455466470124 1103 20 -0.5009758562037376 1103 21 -0.4755331887006875 1103 22 -0.45613205682775515 1103 23 -0.5505456594128262 1103 24 -0.5715351834585718 1103 25 -0.6728546581103458 1104 1 -0.05554697695109212 1104 2 -0.043154703653765836 1104 3 0.2502478963414241 1104 4 0.2022679366495339 1104 5 0.12522718108738218 1104 6 -0.020759883245801802 1104 7 0.14609210788916016 1104 8 0.1410290938620946 1104 9 0.34139895592876 1104 10 0.15217642690929267 1104 11 0.14648288670051812 1104 12 0.27949411834028126 1104 13 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2214 7 -0.20032278698412398 2214 8 -0.046840584833248045 2214 9 -0.09360145778201456 2214 10 -0.30076618136444466 2214 11 -0.5122659715782376 2214 12 -0.3293933531317492 2214 13 -0.3699735235025088 2214 14 -0.4649552684412228 2214 15 -0.4490401955645922 2214 16 -0.33130074248803254 2214 17 -0.3910388808820784 2214 18 -0.5535980705239467 2214 19 -0.599089959708266 2214 20 -0.5024599780601875 2214 21 -0.42836964206602346 2214 22 -0.3167593739814123 2214 23 -0.42544978775465836 2214 24 -0.4864158533635745 2214 25 -0.34231940225247826 2215 1 0.45574083630516427 2215 2 0.38460149427034906 2215 3 0.26021708546767996 2215 4 0.48472308584745605 2215 5 0.43697961888153003 2215 6 0.5115899713734464 2215 7 0.3489007968212334 2215 8 0.4838174505868409 2215 9 0.25281398222701057 2215 10 0.3567081180379006 2215 11 0.40271714623543886 2215 12 0.4577595982061595 2215 13 0.5610474845272719 2215 14 0.39201648798607636 2215 15 0.31600340523128523 2215 16 0.3765197556765614 2215 17 0.45647526481354106 2215 18 0.2987457477715765 2215 19 0.22912043888168654 2215 20 0.35412997720705874 2215 21 0.38361831662772516 2215 22 0.464514138357643 2215 23 0.4321211947594463 2215 24 0.569632706364402 2215 25 0.5144237217776109 2216 1 -0.6717488947651304 2216 2 -0.5721914996070439 2216 3 -2.244355494552469 2216 4 -1.9478894848725332 2216 5 -0.8611293480253863 2216 6 -0.780435139065986 2216 7 -0.8504326915148113 2216 8 -0.476424353644731 2216 9 -0.7103728735429655 2216 10 -0.777485480350073 2216 11 -0.7426366474559529 2216 12 -0.7257636134500416 2216 13 -0.9289548405134198 2216 14 -1.001729152562639 2216 15 -0.9547088322328676 2216 16 -0.8641994569096596 2216 17 -0.8693016862831016 2216 18 -1.035750105905862 2216 19 -0.8903479018142733 2216 20 -0.7455755176390966 2216 21 -0.9916432504496981 2216 22 -1.0261856988895133 2216 23 -1.0379632817188877 2216 24 -1.0751010764767983 2216 25 -1.122211169332985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18445.0 3 15.0 4 300.0 5 2046.0 6 2624.0 7 1962.0 8 1522.0 9 1346.0 10 1148.0 11 1080.0 12 942.0 13 912.0 14 941.0 15 955.0 16 966.0 17 987.0 18 1065.0 19 1293.0 20 1744.0 21 2106.0 22 2837.0 23 3457.0 24 3988.0 25 4772.0 26 5728.0 27 6308.0 28 7202.0 29 8883.0 30 11179.0 31 14626.0 32 19004.0 33 24903.0 34 33798.0 35 46892.0 36 69633.0 37 121968.0 38 286010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34630628608307 19.981620870834004 10.80706824830063 27.865004594782288 2 13.498768286318718 21.02807409361024 41.8194852054072 23.653672414663845 3 18.66820091774751 25.184096114009247 29.08480334582599 27.06289962241726 4 11.4786283942953 17.71052443500232 38.84053381017311 31.970313360529268 5 12.105741121204563 39.27439268786945 36.091171796388224 12.528694394537766 6 30.85451671513672 39.06147165205065 18.06123539570126 12.022776237111373 7 27.845383164156345 31.996737000563456 21.93287864389295 18.225001191387253 8 24.00973759780694 39.80852754571331 19.864951795527265 16.316783060952485 9 25.877195441218515 16.07514253199612 20.399453977721844 37.64820804906352 10 13.848290475516459 30.749718664557058 34.716723587752135 20.68526727217435 11 33.77627483487793 23.30123619077783 24.895909681415656 18.026579292928584 12 22.970558435388735 25.644529087716496 32.37451004298028 19.010402433914486 13 28.809863477498034 22.959099304634925 25.967162848838676 22.263874369028365 14 19.622975194335098 23.878938377520893 28.116403465870317 28.381682962273697 15 21.77386693125141 33.154654547340066 23.413227275211575 21.658251246196944 16 20.426607098813875 29.758935177918787 29.36795107513621 20.446506648131123 17 20.186691405636896 30.287114060853384 28.82365636000807 20.702538173501647 18 19.963956439423136 28.933926211600365 31.41185093212964 19.69026641684686 19 22.50997076736371 27.88577690705815 28.303666271479543 21.300586054098595 20 22.395106365088708 28.71436979735979 29.23862776423106 19.65189607332044 21 21.560138362523265 27.751132464736617 28.018971587468883 22.66975758527123 22 21.570070040611782 29.983211554038334 28.06515262350999 20.381565781839893 23 20.79300883145129 29.4134133797468 28.776629586342782 21.01694820245913 24 21.937431332541088 27.998245476355972 29.7935491827171 20.27077400838584 25 21.24407010069442 28.86068853400977 29.132710620913887 20.762530744381923 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 260.0 1 260.0 2 262.0 3 264.0 4 264.0 5 264.0 6 468.0 7 672.0 8 672.0 9 672.0 10 1043.5 11 1415.0 12 1415.0 13 1415.0 14 2363.0 15 3311.0 16 3311.0 17 3311.0 18 6153.0 19 8995.0 20 8995.0 21 8995.0 22 14400.0 23 19805.0 24 19805.0 25 19805.0 26 28839.0 27 37873.0 28 37873.0 29 37873.0 30 50260.5 31 62648.0 32 62648.0 33 62648.0 34 71388.5 35 80129.0 36 80129.0 37 80129.0 38 85126.0 39 90123.0 40 90123.0 41 90123.0 42 93048.0 43 95973.0 44 95973.0 45 95973.0 46 96093.5 47 96214.0 48 96214.0 49 96214.0 50 89344.5 51 82475.0 52 82475.0 53 82475.0 54 69357.5 55 56240.0 56 56240.0 57 56240.0 58 46488.5 59 36737.0 60 36737.0 61 36737.0 62 29177.5 63 21618.0 64 21618.0 65 21618.0 66 16299.5 67 10981.0 68 10981.0 69 10981.0 70 7968.5 71 4956.0 72 4956.0 73 4956.0 74 3420.0 75 1884.0 76 1884.0 77 1884.0 78 1301.0 79 718.0 80 718.0 81 718.0 82 462.5 83 207.0 84 207.0 85 207.0 86 140.0 87 73.0 88 73.0 89 73.0 90 44.0 91 15.0 92 15.0 93 15.0 94 8.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11519268148102474 2 0.10636404530912139 3 0.01415384529146411 4 0.0 5 0.004624523709092234 6 0.3496420198237916 7 0.01863823191846264 8 0.00896877325399706 9 0.008828636171903355 10 0.003643564134436305 11 0.0033632899702488975 12 5.605483283748163E-4 13 7.006854104685203E-4 14 0.0 15 0.002242193313499265 16 4.204112462811122E-4 17 4.204112462811122E-4 18 8.408224925622244E-4 19 1.4013708209370407E-4 20 9.809595746559285E-4 21 0.013873571127276703 22 1.4013708209370407E-4 23 1.4013708209370407E-4 24 4.204112462811122E-4 25 0.005885757447935571 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 713587.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.6700672644527 #Duplication Level Percentage of deduplicated Percentage of total 1 92.17738511461174 76.20310627686312 2 6.042078044587165 9.989979967261876 3 0.9059800620690793 2.246922980145115 4 0.28269690471387743 0.9348228851259528 5 0.14483333089177639 0.5986690603478945 6 0.09202782605821173 0.45647679422602305 7 0.06297327913963814 0.36442036556359286 8 0.04929215034919691 0.32599883079820985 9 0.03196463321065686 0.23782665398477051 >10 0.17391881470970832 2.7088763672483847 >50 0.020547983557532814 1.1761666994252593 >100 0.013924502091935577 2.5187494295438237 >500 0.001018866004118474 0.6175442384870583 >1k 0.0013584880054912985 1.6204394509788846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1946 0.2727067617543481 No Hit GTATCAACGCAGAGTACTTTTTTTT 1818 0.254769215246354 No Hit CTGTAGGACGTGGAATATGGCAAGA 1789 0.2507052398656366 No Hit GTCCTACAGTGTGCATTTCTCATTT 1417 0.19857424532677864 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1273 0.17839450550528527 No Hit CTTTAGGACGTGAAATATGGCGAGG 1205 0.1688651839229134 No Hit CTGTAGGACCTGGAATATGGCGAGA 1088 0.15246914531795 No Hit GGTATCAACGCAGAGTACTTTTTTT 1007 0.14111804166835998 No Hit TATCAACGCAGAGTACTTTTTTTTT 962 0.1348118729741433 No Hit GTACATGGGAAGCAGTGGTATCAAC 852 0.11939679394383587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTTC 45 1.6883951E-6 18.973303 13 GCCGGTT 60 1.485716E-6 15.811086 11 TAGGACC 300 0.0 13.971517 4 TAGACAG 90 3.4997356E-8 13.759828 5 CCGGTTT 65 5.516725E-5 13.135364 12 GGCGAGG 425 0.0 12.946487 19 CCTAGAC 60 3.9938898E-4 12.70138 3 GTTAGGG 60 3.9938898E-4 12.70138 3 AATCCCG 135 2.7284841E-11 12.648869 19 AGGACCT 520 0.0 12.640314 5 TAGGACT 130 1.8735591E-10 12.457122 4 GTCCTAC 920 0.0 12.437513 1 GGACCTG 515 0.0 12.208121 6 CCTAACA 55 0.0029860584 12.133435 2 TAGGCTA 55 0.0030034317 12.1240425 5 TCCAACG 55 0.003098103 12.073919 18 ATAGTAC 95 1.0034055E-6 12.032885 3 TGTAGGA 935 0.0 12.031473 2 GACGTGA 365 0.0 11.957455 7 CTGTAGG 950 0.0 11.944377 1 >>END_MODULE