FastQCFastQC Report
Thu 26 May 2016
SRR1926982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680003
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10080.14823464014129348No Hit
GTATCAACGCAGAGTACTTTTTTTT9710.14279348767578967No Hit
GTACATGGGAAGCAGTGGTATCAAC8930.13132295004580863No Hit
GTCCTACAGTGTGCATTTCTCATTT8830.12985236829837515No Hit
CTGTAGGACGTGGAATATGGCAAGA8020.11794065614416406No Hit
GTCCTAAAGTGTGTATTTCTCATTT7680.11294067820289029No Hit
CCCATGTACTCTGCGTTGATACCAC7400.10882304931007657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2250.014.8095954
CCTAGAC851.6405465E-814.5757623
AGAACTA551.9270155E-413.8438435
GTCTAAG706.8912304E-613.6379071
GGACGTG4050.013.1601966
GTAGGAC5150.012.5837043
AGGACGT4450.012.4050175
ATAGGAC701.057238E-412.2532493
CTAGGAC701.057238E-412.2532493
TGTAGGA5750.012.1002392
GACGTGA2400.011.8961747
TAGGACA1459.276846E-1111.8184944
TAGGACG4550.011.7174814
CTGTAGG5800.011.68627551
CTAGAAC907.183762E-611.648153
GTCCTAC6150.011.6421161
GGACATG1406.566552E-1011.55713756
TAGACAG1655.456968E-1211.5365365
GTCTAGG751.978626E-411.4558421
CTAGGGC752.0101605E-411.4363673
Produced by FastQC (version 0.11.5)