##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926982_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680003 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.345347005822035 31.0 28.0 31.0 18.0 34.0 2 28.397837950714923 31.0 28.0 31.0 18.0 34.0 3 28.187022704311598 31.0 28.0 33.0 16.0 34.0 4 31.752580503321308 35.0 32.0 37.0 19.0 37.0 5 31.99447061262965 35.0 33.0 37.0 19.0 37.0 6 31.15452137711157 35.0 32.0 37.0 16.0 37.0 7 31.226673999967648 35.0 32.0 37.0 17.0 37.0 8 31.04002923516514 35.0 32.0 37.0 11.0 37.0 9 33.236135722930634 37.0 33.0 39.0 17.0 39.0 10 33.41276435545137 37.0 34.0 39.0 17.0 39.0 11 33.57910038632183 38.0 34.0 39.0 19.0 39.0 12 33.44657302982487 38.0 34.0 39.0 18.0 39.0 13 33.561510758040775 38.0 34.0 39.0 19.0 39.0 14 34.58814299348679 39.0 34.0 41.0 19.0 41.0 15 34.15978311860389 39.0 34.0 41.0 17.0 41.0 16 34.24683861688845 39.0 34.0 41.0 17.0 41.0 17 34.47424790772982 39.0 34.0 41.0 17.0 41.0 18 34.53517410952599 39.0 34.0 41.0 18.0 41.0 19 33.91153568440139 39.0 33.0 41.0 10.0 41.0 20 34.18833299264856 39.0 34.0 41.0 16.0 41.0 21 34.18638888357845 39.0 34.0 41.0 11.0 41.0 22 34.19970353071972 39.0 34.0 41.0 10.0 41.0 23 34.195922665047064 39.0 34.0 41.0 7.0 41.0 24 32.907625407534965 39.0 32.0 41.0 2.0 41.0 25 33.4909022460195 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.14969059344379332 1101 2 -0.23664908389932293 1101 3 -1.000592020967506 1101 4 -0.337433790891577 1101 5 -0.21284536081052963 1101 6 -1.8628097802609282 1101 7 -0.3738232808916493 1101 8 -0.06334134490076337 1101 9 -0.1989799239488761 1101 10 -8.997746557882778 1101 11 -3.923226886974515 1101 12 -1.3373643387725949 1101 13 -0.5935116594279108 1101 14 -0.3722617268011845 1101 15 -2.113194237963036 1101 16 -0.3324092615571459 1101 17 0.06965011108870556 1101 18 -0.063773961827998 1101 19 -0.6870754668538268 1101 20 0.17768740595442267 1101 21 0.1575637897009159 1101 22 0.2986412891998782 1101 23 0.13595094511178019 1101 24 -0.14341753951267577 1101 25 0.2211288117170369 1102 1 0.11273987881343572 1102 2 -0.09243222313701338 1102 3 -0.2034657499826551 1102 4 -0.17798181374779531 1102 5 0.2190976707255814 1102 6 -0.860658785489111 1102 7 0.3364716331444768 1102 8 0.688906571164889 1102 9 0.6636104810960859 1102 10 0.5561776157556437 1102 11 0.22142422167349451 1102 12 0.20554269270278525 1102 13 -0.05664057234045572 1102 14 0.008865192012684986 1102 15 0.46176919512583936 1102 16 0.28634336392176607 1102 17 0.11434727633065478 1102 18 -0.07112175996851988 1102 19 0.26422601151222835 1102 20 0.18239226416591237 1102 21 -0.03557630838444226 1102 22 -0.04558670612360771 1102 23 -0.2489303452826377 1102 24 -3.2214353851775392 1102 25 -1.5055511151997543 1103 1 0.12243475177400498 1103 2 -0.20438654887903596 1103 3 0.14715920287320827 1103 4 -0.051762946715662395 1103 5 -0.1851900501892878 1103 6 0.7747289788493319 1103 7 0.2555508903363801 1103 8 0.6472260585628824 1103 9 0.2304919551326705 1103 10 0.1549020228607958 1103 11 -0.03762184604688912 1103 12 -0.20593132348829357 1103 13 -0.1313505212311199 1103 14 -0.052825840102414645 1103 15 0.3013313305869971 1103 16 0.30862903393931873 1103 17 -0.02442161445193136 1103 18 -0.04899714451177317 1103 19 -1.6500511695029019 1103 20 -0.4137511926357931 1103 21 -0.09034097505247018 1103 22 -0.11148044680945901 1103 23 -0.21531377337992552 1103 24 1.5361306115157447 1103 25 0.9114966119942096 1104 1 -0.14668108303903793 1104 2 -0.27714503244735056 1104 3 -1.1358606679096148 1104 4 -0.4737365599539274 1104 5 -0.23092001699913567 1104 6 -1.7038745937181972 1104 7 -0.2278087562652722 1104 8 0.13482010717090276 1104 9 0.09754433100425075 1104 10 0.19851183580692577 1104 11 0.15145178254625336 1104 12 0.10549112848835307 1104 13 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10 -0.11684989397478773 1107 11 -0.3336594279027594 1107 12 0.017460009847596325 1107 13 -0.4131933656197049 1107 14 -0.08213302042864967 1107 15 -1.2614538956973078 1107 16 -0.6235872150021606 1107 17 -0.4534000301743859 1107 18 -0.6182178038035886 1107 19 0.21691795038122308 1107 20 0.25123077895557344 1107 21 -0.03667724223310387 1107 22 -0.30524273237485033 1107 23 -0.35284313420483215 1107 24 -3.6858582993844493 1107 25 -2.173909381637415 1108 1 0.5830074980197928 1108 2 0.12139684465001821 1108 3 0.8940843148674418 1108 4 0.7988761846544179 1108 5 0.9071169744060441 1108 6 -2.5110516219001866 1108 7 -1.3322008843995334 1108 8 0.4619024924098589 1108 9 0.3989569453768098 1108 10 0.9078806731934748 1108 11 0.7845309340896591 1108 12 0.6837953992604042 1108 13 0.6353693592077292 1108 14 0.4595340963429848 1108 15 -0.34546508710715074 1108 16 0.48207762603877313 1108 17 0.39401978812723826 1108 18 0.5054224290987293 1108 19 0.5531499596857969 1108 20 -0.8894936379784539 1108 21 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19 0.9138796117676691 1111 20 0.46083008671122627 1111 21 0.5063926381593546 1111 22 0.5558670218504886 1111 23 0.4190790999579903 1111 24 1.0404714099008388 1111 25 0.6455523146403763 1112 1 -0.34775771843053604 1112 2 -0.37813627260823424 1112 3 -1.9731490689066788 1112 4 -0.795048405492345 1112 5 -0.63379068055103 1112 6 0.36339008233526826 1112 7 0.4754364445773902 1112 8 0.7331866730262853 1112 9 -0.4227793697227895 1112 10 -0.1991768485056582 1112 11 -0.24028017475249186 1112 12 -0.2654182305740136 1112 13 -0.4642285107597601 1112 14 -0.26567879346047363 1112 15 0.17099187503084323 1112 16 0.06664274440009166 1112 17 -0.7324967081054083 1112 18 -0.657484740649366 1112 19 0.27991534369030546 1112 20 -0.005825981694911775 1112 21 -0.16809982918334754 1112 22 -0.09904036739204969 1112 23 -0.03436522196755476 1112 24 1.1977727094279729 1112 25 0.3548448469256087 1113 1 -0.48087259552131556 1113 2 -0.0030314577443846247 1113 3 -4.4567207181409785 1113 4 -1.0576923880158482 1113 5 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-0.04403307655688593 2215 1 0.5956177990913503 2215 2 0.2323813099827916 2215 3 0.480530749459831 2215 4 0.8206535019020933 2215 5 0.6290760365693799 2215 6 1.5657722006641421 2215 7 0.8529815595350421 2215 8 -0.07462988038926 2215 9 0.4724098520618938 2215 10 0.21285110880561575 2215 11 0.10762322925656065 2215 12 -0.3508167274840588 2215 13 0.2206924292987651 2215 14 0.18936246664208056 2215 15 0.2510587329900602 2215 16 0.5714122422773897 2215 17 -0.052034044552307535 2215 18 -0.3597960348689071 2215 19 0.4386843801991205 2215 20 -3.651906980193438 2215 21 -1.7986116649037882 2215 22 -0.739760587877619 2215 23 -0.848115621617886 2215 24 -0.8314359626806791 2215 25 -0.6397806149487621 2216 1 -0.7422859788859206 2216 2 -1.309361306757161 2216 3 -0.6031515231083695 2216 4 -0.6208121950380914 2216 5 -0.9370613886850947 2216 6 -1.8238235268589484 2216 7 -10.590660696600562 2216 8 -11.465769968334119 2216 9 -6.387587409170852 2216 10 -3.9169961288433086 2216 11 -2.716561607102097 2216 12 -2.4740407129990203 2216 13 -2.200513236906371 2216 14 -2.1934380750252203 2216 15 -3.43308000078655 2216 16 -2.3890468055445275 2216 17 -2.5410576740275275 2216 18 -2.2132088431704133 2216 19 -1.7069716306342286 2216 20 -2.670798388071958 2216 21 -5.281400795040877 2216 22 -3.7915636096508187 2216 23 -3.5772903397530946 2216 24 -3.8603208279485486 2216 25 -3.0566400096811996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37926.0 3 2.0 4 269.0 5 1711.0 6 2271.0 7 1893.0 8 1539.0 9 1346.0 10 1328.0 11 1282.0 12 1209.0 13 1169.0 14 1252.0 15 1222.0 16 1373.0 17 1475.0 18 1724.0 19 2010.0 20 2718.0 21 3493.0 22 4232.0 23 5123.0 24 6105.0 25 6905.0 26 8241.0 27 8911.0 28 9769.0 29 11685.0 30 13726.0 31 16542.0 32 21681.0 33 28384.0 34 38239.0 35 53963.0 36 78366.0 37 125404.0 38 175515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1581709134378 18.64727307030719 12.509636119492304 25.684919896762704 2 14.227591396297765 20.33962447522688 41.71237647308288 23.72040765539248 3 18.781301309850758 24.351148972514487 29.773739664401365 27.093810053233398 4 11.990852339452376 16.615012405852383 38.588676090993914 32.80545916370133 5 12.595867863415322 37.933814033260674 36.544079359615914 12.926238743708096 6 31.227467356625827 37.069958387696055 19.24285905505209 12.459715200626023 7 27.778187471055173 31.1958160155985 22.55381157321235 18.472184940133978 8 24.495632179839447 36.94174420782653 21.676570402063636 16.886053210270386 9 25.994713687489796 15.604476744784657 20.43673119380351 37.964078373922035 10 14.204192717910995 29.524678190511217 35.04450165502023 21.226627436557557 11 34.04879641586972 23.08033993934277 24.320547856552423 18.550315788235086 12 22.77206024030817 25.269570819427635 32.05035616736557 19.90801277289863 13 28.54081451051857 22.293213785110304 25.765524869354444 23.400446835016687 14 20.285551231250313 22.59302404254881 27.981343711665918 29.140081014534964 15 22.995349275034048 31.21354211097612 23.24774155971372 22.54336705427611 16 21.61441824137581 28.711751372707067 28.292548035554326 21.381282350362795 17 21.3377028154036 29.096867475550365 28.04283779033252 21.522591918713513 18 21.032280079365428 28.270036608701666 29.740415035895357 20.957268276037553 19 23.242879879499995 27.338732690627417 27.761482950249622 21.65690447962297 20 23.00720427608645 27.896198413235396 28.309657377690574 20.786939932987583 21 22.582637881787456 27.288224340853134 27.33176053914287 22.797377238216544 22 22.403038178740104 28.479248574382872 27.594682010528317 21.523031236348714 23 21.641049024078132 28.20539220733376 28.060511568388076 22.09304720020004 24 22.765808486103595 27.44554985527028 28.374232226484363 21.414409432141763 25 22.171053889520525 27.637665449314667 28.288505069194912 21.90277559196989 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 245.0 3 306.0 4 306.0 5 306.0 6 481.0 7 656.0 8 656.0 9 656.0 10 990.5 11 1325.0 12 1325.0 13 1325.0 14 2242.0 15 3159.0 16 3159.0 17 3159.0 18 5613.0 19 8067.0 20 8067.0 21 8067.0 22 12816.5 23 17566.0 24 17566.0 25 17566.0 26 24997.0 27 32428.0 28 32428.0 29 32428.0 30 42278.5 31 52129.0 32 52129.0 33 52129.0 34 60209.5 35 68290.0 36 68290.0 37 68290.0 38 74287.0 39 80284.0 40 80284.0 41 80284.0 42 83755.5 43 87227.0 44 87227.0 45 87227.0 46 88914.5 47 90602.0 48 90602.0 49 90602.0 50 86366.5 51 82131.0 52 82131.0 53 82131.0 54 71984.5 55 61838.0 56 61838.0 57 61838.0 58 52404.5 59 42971.0 60 42971.0 61 42971.0 62 34655.5 63 26340.0 64 26340.0 65 26340.0 66 20251.0 67 14162.0 68 14162.0 69 14162.0 70 10287.0 71 6412.0 72 6412.0 73 6412.0 74 4490.5 75 2569.0 76 2569.0 77 2569.0 78 1737.0 79 905.0 80 905.0 81 905.0 82 610.0 83 315.0 84 315.0 85 315.0 86 209.5 87 104.0 88 104.0 89 104.0 90 65.0 91 26.0 92 26.0 93 26.0 94 15.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23029310164808095 2 0.13235235726901204 3 0.1349994044143923 4 0.07132321475052317 5 0.023676366133678822 6 0.02294107525996209 7 0.2924987095645166 8 0.2905869532928531 9 0.01970579541560846 10 0.03720571821006672 11 0.017205806444971567 12 0.018529330017661685 13 0.01617639922176814 14 0.01838227184291834 15 0.016029341047024793 16 0.020882260813555233 17 0.019999911765095153 18 0.01617639922176814 19 0.024852831531625593 20 0.01897050454189173 21 0.016029341047024793 22 0.017941097318688298 23 0.019558737240865116 24 0.016911690095484874 25 0.016470515571254833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 680003.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.60365860417674 #Duplication Level Percentage of deduplicated Percentage of total 1 93.12182303217338 80.64690570476907 2 5.29971458955521 9.179493460268283 3 0.8454444168554078 2.196557389384591 4 0.2609930494773355 0.9041181181999268 5 0.13245028111828994 0.5735339463997811 6 0.07574921507808355 0.3936095497294023 7 0.04918457752996501 0.29816950526970265 8 0.03807381844443271 0.26378655794552464 9 0.029643867956449202 0.23105406781868593 >10 0.1236318226950598 1.9490914540993256 >50 0.012070898595904342 0.7083534940468681 >100 0.009520365292319904 1.5055930899375647 >500 0.0015300587054017122 1.0013239385523165 >1k 1.7000652282241245E-4 0.14840972357899354 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1008 0.14823464014129348 No Hit GTATCAACGCAGAGTACTTTTTTTT 971 0.14279348767578967 No Hit GTACATGGGAAGCAGTGGTATCAAC 893 0.13132295004580863 No Hit GTCCTACAGTGTGCATTTCTCATTT 883 0.12985236829837515 No Hit CTGTAGGACGTGGAATATGGCAAGA 802 0.11794065614416406 No Hit GTCCTAAAGTGTGTATTTCTCATTT 768 0.11294067820289029 No Hit CCCATGTACTCTGCGTTGATACCAC 740 0.10882304931007657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4705817474334673E-4 2 0.0 0.0 0.0 0.0 1.4705817474334673E-4 3 0.0 0.0 0.0 0.0 1.4705817474334673E-4 4 0.0 0.0 0.0 0.0 1.4705817474334673E-4 5 0.0 0.0 0.0 0.0 1.4705817474334673E-4 6 0.0 0.0 0.0 0.0 1.4705817474334673E-4 7 0.0 0.0 0.0 0.0 1.4705817474334673E-4 8 0.0 0.0 0.0 0.0 1.4705817474334673E-4 9 0.0 0.0 0.0 0.0 1.4705817474334673E-4 10 0.0 0.0 0.0 0.0 1.4705817474334673E-4 11 0.0 0.0 0.0 0.0 1.4705817474334673E-4 12 0.0 0.0 0.0 0.0 2.9411634948669347E-4 13 0.0 0.0 0.0 0.0 2.9411634948669347E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 225 0.0 14.809595 4 CCTAGAC 85 1.6405465E-8 14.575762 3 AGAACTA 55 1.9270155E-4 13.843843 5 GTCTAAG 70 6.8912304E-6 13.637907 1 GGACGTG 405 0.0 13.160196 6 GTAGGAC 515 0.0 12.583704 3 AGGACGT 445 0.0 12.405017 5 ATAGGAC 70 1.057238E-4 12.253249 3 CTAGGAC 70 1.057238E-4 12.253249 3 TGTAGGA 575 0.0 12.100239 2 GACGTGA 240 0.0 11.896174 7 TAGGACA 145 9.276846E-11 11.818494 4 TAGGACG 455 0.0 11.717481 4 CTGTAGG 580 0.0 11.6862755 1 CTAGAAC 90 7.183762E-6 11.64815 3 GTCCTAC 615 0.0 11.642116 1 GGACATG 140 6.566552E-10 11.5571375 6 TAGACAG 165 5.456968E-12 11.536536 5 GTCTAGG 75 1.978626E-4 11.455842 1 CTAGGGC 75 2.0101605E-4 11.436367 3 >>END_MODULE