FastQCFastQC Report
Thu 26 May 2016
SRR1926982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680003
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15160.22294019291091363No Hit
GTCCTACAGTGGACATTTCTAAATT10730.15779342149961104No Hit
CTGTAGGACGTGGAATATGGCAAGA10140.14911698918975358No Hit
GTACATGGGAAGCAGTGGTATCAAC9920.14588170934539993No Hit
GGTATCAACGCAGAGTACTTTTTTT9770.14367583672424974No Hit
GTCCTACAGTGTGCATTTCTCATTT9000.13235235726901204No Hit
CCCATGTACTCTGCGTTGATACCAC8480.12470533218235803No Hit
CTTTAGGACGTGAAATATGGCGAGG8410.12367592495915458No Hit
TATCAACGCAGAGTACTTTTTTTTT8270.12161711051274773No Hit
GTCCTAAAGTGTGTATTTCTCATTT7980.11735242344519069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG350.002137654516.3206835
TCGAACT601.4818033E-615.81482819
GCCTCGA551.1389999E-515.52728516
GATTAGG400.00515737814.2985291
ATTGGAC400.00520050914.2805973
TAGGACC2850.014.0300614
TTAGACT551.9222917E-413.8478524
GTCTAAG655.272713E-513.1986431
AGGACTG1259.822543E-1112.94774155
TGCACCT701.0675599E-412.2405125
CTCGAAC701.10111534E-412.2000118
TATGGGC550.003016527512.116873
TAGGCAG1103.6874553E-812.116875
GGTCATT550.00309235912.07677711
AGGACCT5000.011.8052945
GTATTAG657.787827E-411.7321271
GGCGAGA4050.011.71468719
CTAGAAC907.267483E-611.6360433
TTAGGAC2700.011.6360433
GGACCTG4700.011.5460166
Produced by FastQC (version 0.11.5)