FastQCFastQC Report
Thu 26 May 2016
SRR1926981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756711
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12200.1612240340103421No Hit
GTATCAACGCAGAGTACTTTTTTTT11600.15329498315737447No Hit
GTCCTACAGTGTGCATTTCTCATTT8720.11523553906312978No Hit
CTGTAGGACGTGGAATATGGCAAGA8700.11497123736803087No Hit
GTCCTAAAGTGTGTATTTCTCATTT7750.10241690685083209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG1500.013.90929319
GTCCTAT1101.7462298E-1013.8731961
TCCAACG551.986951E-413.7943418
GTCCTAC5800.012.4978361
CCTACAC1457.2759576E-1212.4887053
GTCCTAA3850.012.3867821
GTCCCTA701.04978506E-412.2629151
GGATAGT701.0714882E-412.2360746
GGACCTG3200.012.1935886
CCTAACC550.002992358512.1301773
AATGCCG550.00310585112.07004718
TCCTACA6500.012.0235392
ATTATAC802.7683554E-511.9135673
GGACGTG4000.011.8961836
TGGATAG802.810053E-511.8961835
GACGTGG2350.011.7443167
CCTGGAC1552.3646862E-1111.6829823
CTGGACA1552.3646862E-1111.669034
TAGGACC2450.011.6564964
TAGGACG4350.011.5984374
Produced by FastQC (version 0.11.5)