##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 756711 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30785068540037 31.0 28.0 31.0 18.0 34.0 2 28.338427748506366 31.0 28.0 31.0 18.0 34.0 3 28.139054407825444 31.0 28.0 31.0 16.0 34.0 4 31.678528526742706 35.0 32.0 37.0 19.0 37.0 5 31.980084867274297 35.0 33.0 37.0 19.0 37.0 6 31.124338089442336 35.0 32.0 37.0 17.0 37.0 7 31.190499411267975 35.0 32.0 37.0 17.0 37.0 8 30.98380623514129 35.0 32.0 37.0 11.0 37.0 9 33.22081745871277 37.0 33.0 39.0 17.0 39.0 10 33.404483349653965 37.0 34.0 39.0 17.0 39.0 11 33.568720422988434 38.0 34.0 39.0 19.0 39.0 12 33.454026702400256 38.0 34.0 39.0 18.0 39.0 13 33.56609855017305 38.0 34.0 39.0 19.0 39.0 14 34.594985403938885 39.0 34.0 41.0 19.0 41.0 15 34.16745891099772 39.0 34.0 41.0 17.0 41.0 16 34.2543044834818 39.0 34.0 41.0 17.0 41.0 17 34.48770798891519 39.0 34.0 41.0 18.0 41.0 18 34.53798213584843 39.0 34.0 41.0 18.0 41.0 19 33.94997561816863 39.0 34.0 41.0 10.0 41.0 20 34.22159054117093 39.0 34.0 41.0 16.0 41.0 21 34.20444529020987 39.0 34.0 41.0 11.0 41.0 22 34.22404722542688 39.0 34.0 41.0 10.0 41.0 23 34.2217702663236 39.0 34.0 41.0 8.0 41.0 24 32.96578482406097 39.0 32.0 41.0 2.0 41.0 25 33.53368723330307 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.21074049375358683 1101 2 -0.2846960319486165 1101 3 -1.1167413982985614 1101 4 -0.6215484377373635 1101 5 -0.15980323267226026 1101 6 -2.698793534700318 1101 7 -0.5877419111165629 1101 8 -0.2117696224601957 1101 9 -0.1482259819857248 1101 10 -9.554378177247212 1101 11 -4.295544926719124 1101 12 -1.6966587353247746 1101 13 -0.9227981241226217 1101 14 -0.6998657478841679 1101 15 -1.824747751213124 1101 16 -0.20441879310202893 1101 17 -0.1369994565422843 1101 18 0.11540133749549142 1101 19 -0.951127607981185 1101 20 0.04168249172683147 1101 21 0.06158851840189783 1101 22 0.340375419184177 1101 23 -0.003481321842976115 1101 24 0.07078536318039141 1101 25 0.13023116408418645 1102 1 -0.5507304384494134 1102 2 -0.6689454034921063 1102 3 -0.8247273208727215 1102 4 -0.5490899158670786 1102 5 -0.42000232769488477 1102 6 -1.196708064614846 1102 7 -0.5671245154403408 1102 8 -0.1917957662510439 1102 9 -0.21323151240301996 1102 10 -0.25519265688522097 1102 11 -0.4140084762414986 1102 12 -0.4544304799200489 1102 13 -0.38859199038707715 1102 14 -0.7349426709610896 1102 15 -0.21480506644691388 1102 16 -0.38356007012566096 1102 17 -0.4914730613688292 1102 18 -0.6814050978991801 1102 19 -0.27675857831803796 1102 20 -0.26541856408010744 1102 21 -0.44779308039146315 1102 22 -0.44166982968459934 1102 23 -0.7557789588464985 1102 24 -3.952390101877427 1102 25 -2.2403791928791144 1103 1 0.3430161200030284 1103 2 0.1477272484746628 1103 3 0.4420734165162514 1103 4 0.06938630653071343 1103 5 0.12995338108435206 1103 6 0.8704198691797558 1103 7 0.5800252846506346 1103 8 0.5437577144004742 1103 9 0.32798565822591286 1103 10 0.5956041860684849 1103 11 0.26552738368651774 1103 12 0.031693998361291165 1103 13 0.257110165119002 1103 14 0.44016247081071924 1103 15 0.6865891094570671 1103 16 0.621443640760404 1103 17 0.3936107727346112 1103 18 0.1214965755907329 1103 19 -1.4979353681222776 1103 20 -0.005534439490098464 1103 21 0.008008271488321839 1103 22 0.2457299165386786 1103 23 0.14279733776901793 1103 24 1.6253038816989118 1103 25 1.007599770786122 1104 1 -0.6714602019267097 1104 2 -0.6122385736029621 1104 3 -1.5347250323318846 1104 4 -0.846132880210007 1104 5 -0.8711146789899189 1104 6 -1.990524186381279 1104 7 -0.796506970763609 1104 8 -0.4110660830734254 1104 9 -0.5672294326413478 1104 10 -0.5843338116748953 1104 11 -0.4513654926266497 1104 12 -0.3945879475893932 1104 13 -0.6515734371463822 1104 14 -0.6633298117705877 1104 15 -0.3861485202163806 1104 16 -0.747546467360138 1104 17 -0.6238944580211339 1104 18 -0.8050537546863055 1104 19 -1.3726163194077827 1104 20 -0.8035396319052239 1104 21 -0.6053265954697338 1104 22 -0.624295388269239 1104 23 -0.4981318197232909 1104 24 0.8657817153444469 1104 25 0.23196359038804104 1105 1 -0.17699049375358555 1105 2 -0.226446031948619 1105 3 0.3546891572569919 1105 4 -0.39102760440403017 1105 5 -0.0402268437833726 1105 6 -0.5464810347003173 1105 7 -0.15220024444989377 1105 8 0.41349426642869247 1105 9 -0.7922607042079477 1105 10 0.010934322752788717 1105 11 -0.06918381560801379 1105 12 -0.2106726242136645 1105 13 -0.07813840190039656 1105 14 -0.5641226923286098 1105 15 0.20525919323132058 1105 16 -0.5016549042131402 1105 17 -0.42333973432005934 1105 18 -0.30757088472672933 1105 19 -0.8680026079811896 1105 20 -0.23403973049538962 1105 21 -0.2624392593758813 1105 22 -0.1960690252602646 1105 23 -0.4482382662874187 1105 24 -5.065957692375164 1105 25 -2.340421613693593 1106 1 -0.3185038415169359 1106 2 -0.9644420636946514 1106 3 -0.3903460159031802 1106 4 -0.6419813381702646 1106 5 -0.20458245345148285 1106 6 -0.007630475537258263 1106 7 -2.9956841910588423 1106 8 -0.3754983381889119 1106 9 -0.7415189112786535 1106 10 -0.13134787421690675 1106 11 -0.4064944216686186 1106 12 -0.1845317511977882 1106 13 -0.46647489179938617 1106 14 -0.7192250552434771 1106 15 -0.0831719936373645 1106 16 -0.032620813304049534 1106 17 -0.4754929630357907 1106 18 -0.6380993840052298 1106 19 -0.18772212457569992 1106 20 -0.15526988922554352 1106 21 -0.5472426504292685 1106 22 -0.8169925461837906 1106 23 -0.8026732410348956 1106 24 -3.716735560340531 1106 25 -1.934022804169782 1107 1 0.3120929823960843 1107 2 0.20892064899968332 1107 3 0.5315716551147496 1107 4 0.4330873806002522 1107 5 0.5020579498470177 1107 6 0.3214383990329033 1107 7 -1.5019172895798576 1107 8 -1.3623688798152358 1107 9 -0.07329424819538133 1107 10 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1108 22 0.3322737242689229 1108 23 0.2202851564997701 1108 24 -0.6453803618666925 1108 25 0.02509745410301889 1109 1 0.5424592428161752 1109 2 0.3072324655582044 1109 3 -0.9320602429688059 1109 4 0.4030183136033081 1109 5 0.680887707209191 1109 6 0.7856667155584098 1109 7 0.9475754282380322 1109 8 1.2903479803246398 1109 9 0.6662762289466286 1109 10 0.6731578092049446 1109 11 0.23953231478995463 1109 12 -0.042851980220817154 1109 13 0.2455051969800195 1109 14 0.19931122078644847 1109 15 -0.8853427272221595 1109 16 0.24610035263133767 1109 17 0.11494070716079108 1109 18 0.21457077958788062 1109 19 -0.898204473161357 1109 20 -0.11736012752238878 1109 21 0.12325377383514535 1109 22 0.5493915072451401 1109 23 0.3934779405523585 1109 24 -0.5237066868713569 1109 25 0.005140939227658237 1110 1 0.5095372840241907 1110 2 0.6234150791624913 1110 3 0.8353141572569918 1110 4 0.6326182289292994 1110 5 0.8520301006610715 1110 6 0.917650909744129 1110 7 0.9498136444389971 1110 8 1.2246748219842516 1110 9 0.960829573569832 1110 10 0.8081218227527884 1110 11 0.5945661843919865 1110 12 0.5668968202307809 1110 13 0.5789796536551535 1110 14 0.3006342521158345 1110 15 0.7759189154535449 1110 16 0.6189700957868567 1110 17 0.3318338767910518 1110 18 0.44562355971771694 1110 19 -1.3391831635367453 1110 20 0.1415158250601678 1110 21 0.16492185173522955 1110 22 0.22004208585084228 1110 23 0.0434631226014659 1110 24 -1.036270192375163 1110 25 -0.7693799470269269 1111 1 0.2121654891523974 1111 2 -0.13407458171426612 1111 3 0.525245919043595 1111 4 0.5153580965885247 1111 5 0.5546583057892782 1111 6 0.8009759717788683 1111 7 0.6325349016763795 1111 8 0.7927806946062503 1111 9 0.3603684486732206 1111 10 0.18514208743434324 1111 11 0.024876358089258588 1111 12 0.2844981436385581 1111 13 0.2369614567982481 1111 14 0.16563631994048222 1111 15 -0.8622531275572101 1111 16 -0.36769863870446073 1111 17 -0.24426827374658444 1111 18 -0.011828880315370327 1111 19 0.6073945866700399 1111 20 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-5.8875584573182 2216 10 -3.5054206482896824 2216 11 -2.537885553059752 2216 12 -2.306024672690711 2216 13 -2.0409688662933654 2216 14 -2.235803971822392 2216 15 -3.3609536714190433 2216 16 -2.3209559016391417 2216 17 -2.4297459172887415 2216 18 -2.3903867362925766 2216 19 -1.8580859949395716 2216 20 -2.5158876498433074 2216 21 -4.970174673361292 2216 22 -3.6647519939432343 2216 23 -3.5811218194834744 2216 24 -4.116337759942731 2216 25 -3.622311092458073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42248.0 3 4.0 4 323.0 5 1898.0 6 2579.0 7 2092.0 8 1588.0 9 1470.0 10 1435.0 11 1360.0 12 1392.0 13 1313.0 14 1407.0 15 1458.0 16 1512.0 17 1608.0 18 1826.0 19 2248.0 20 3066.0 21 3825.0 22 4769.0 23 5781.0 24 6783.0 25 7680.0 26 8714.0 27 9650.0 28 10620.0 29 12995.0 30 15395.0 31 18743.0 32 24174.0 33 31967.0 34 43259.0 35 60451.0 36 88321.0 37 141577.0 38 191180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72403180438502 19.24631447353784 12.821341356824648 25.20831236525249 2 13.695798875289448 20.563810783989414 43.11465431690374 22.6257360238174 3 18.697203226557704 24.72820909110156 29.8775485359812 26.697039146359536 4 11.400854773188897 17.034238532498307 39.96765508632363 31.59725160798917 5 12.146273955691141 38.30822163153143 37.088346751648594 12.45715766112883 6 29.971740360264143 38.23827978365117 19.895156472224134 11.894823383860546 7 27.470772248879932 31.421887012539436 22.85000397656478 18.257336762015854 8 23.596451234963872 38.063738873662054 22.10238086402409 16.237429027349982 9 25.65164349902919 16.18769553270146 21.021640604451306 37.13902036381804 10 13.14221202250518 30.61823152417615 36.015430009729684 20.224126443588986 11 33.598334655035686 23.566745968807826 24.628998149616706 18.205921226539783 12 21.887168018778898 25.997562761533928 33.00264078224252 19.112628437444652 13 28.03272513398669 22.89543420939592 26.377436046550052 22.69440461006734 14 19.399593966597408 23.31268801514166 29.14968252205956 28.13803549620137 15 22.19006118087414 32.110905368558925 23.99337041986019 21.705663030706745 16 20.59980438288086 30.033307779745698 28.92529540828465 20.441592429088796 17 20.350759860742865 29.86798592625293 28.84179723124668 20.939456981757527 18 19.86579647163936 29.51103075288259 30.5618996262285 20.06127314924955 19 22.19932561487084 28.216448282379936 28.610346299803975 20.97387980294525 20 21.860543804090955 28.706205161010633 29.663915748959813 19.7693352859386 21 21.77731019132862 28.090388102627 28.447774529344283 21.684527176700097 22 21.207627370666582 29.446689162308303 29.02849756341273 20.317185903612387 23 20.994231968955198 28.861038562803337 28.813720617935353 21.331008850306112 24 21.449171158263702 28.32613884784258 29.79521489615357 20.429475097740145 25 21.36309987192687 28.518277185140345 29.158510650952486 20.960112291980295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 163.0 2 212.0 3 261.0 4 261.0 5 261.0 6 510.5 7 760.0 8 760.0 9 760.0 10 1251.0 11 1742.0 12 1742.0 13 1742.0 14 3042.5 15 4343.0 16 4343.0 17 4343.0 18 7569.5 19 10796.0 20 10796.0 21 10796.0 22 16773.5 23 22751.0 24 22751.0 25 22751.0 26 32180.5 27 41610.0 28 41610.0 29 41610.0 30 53516.5 31 65423.0 32 65423.0 33 65423.0 34 74811.0 35 84199.0 36 84199.0 37 84199.0 38 90690.5 39 97182.0 40 97182.0 41 97182.0 42 100159.0 43 103136.0 44 103136.0 45 103136.0 46 102341.5 47 101547.0 48 101547.0 49 101547.0 50 94544.5 51 87542.0 52 87542.0 53 87542.0 54 74432.5 55 61323.0 56 61323.0 57 61323.0 58 49942.0 59 38561.0 60 38561.0 61 38561.0 62 29487.0 63 20413.0 64 20413.0 65 20413.0 66 14912.5 67 9412.0 68 9412.0 69 9412.0 70 6571.5 71 3731.0 72 3731.0 73 3731.0 74 2501.5 75 1272.0 76 1272.0 77 1272.0 78 833.5 79 395.0 80 395.0 81 395.0 82 251.5 83 108.0 84 108.0 85 108.0 86 67.0 87 26.0 88 26.0 89 26.0 90 18.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 2.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21078060184138991 2 0.12699696449503178 3 0.12990428314111993 4 0.0643574627565874 5 0.0208798339128148 6 0.020483381370166417 7 0.30275759173581457 8 0.29007111037106637 9 0.016386705096133134 10 0.03475567290550818 11 0.014668744077990143 12 0.01546164916328691 13 0.014007989840242839 14 0.016254554248583675 15 0.013479386450044997 16 0.01731176102897936 17 0.016518855943682593 18 0.013082933907396614 19 0.02246564408340833 20 0.015197347468187987 21 0.014140140687792301 22 0.01585810170593529 23 0.016783157638781517 24 0.014933045773089066 25 0.014800894925539605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 756711.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.32559943136371 #Duplication Level Percentage of deduplicated Percentage of total 1 95.02608356726793 84.88261876261069 2 3.9433098540707916 7.044770329169538 3 0.5024664318174005 1.3464934564868323 4 0.15265709551025133 0.5454474625561657 5 0.09385068028059113 0.41916341365525345 6 0.05903877503940111 0.3164204382052763 7 0.03707004249488896 0.23179126367614575 8 0.030255170314645124 0.21620489794029413 9 0.019960449954544764 0.16046812413985032 >10 0.11518417250166164 1.9018275590048077 >50 0.011542149099686073 0.7062263290064649 >100 0.006954064639237192 1.0836216850485871 >500 0.001331629371021652 0.8303933357746793 >1k 2.959176380048115E-4 0.31455294272540774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1220 0.1612240340103421 No Hit GTATCAACGCAGAGTACTTTTTTTT 1160 0.15329498315737447 No Hit GTCCTACAGTGTGCATTTCTCATTT 872 0.11523553906312978 No Hit CTGTAGGACGTGGAATATGGCAAGA 870 0.11497123736803087 No Hit GTCCTAAAGTGTGTATTTCTCATTT 775 0.10241690685083209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3215084754946075E-4 0.0 0.0 0.0 0.0 8 1.3215084754946075E-4 0.0 0.0 0.0 0.0 9 1.3215084754946075E-4 0.0 0.0 0.0 0.0 10 1.3215084754946075E-4 0.0 0.0 0.0 0.0 11 1.3215084754946075E-4 0.0 0.0 0.0 0.0 12 1.3215084754946075E-4 0.0 0.0 0.0 0.0 13 1.3215084754946075E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 150 0.0 13.909293 19 GTCCTAT 110 1.7462298E-10 13.873196 1 TCCAACG 55 1.986951E-4 13.79434 18 GTCCTAC 580 0.0 12.497836 1 CCTACAC 145 7.2759576E-12 12.488705 3 GTCCTAA 385 0.0 12.386782 1 GTCCCTA 70 1.04978506E-4 12.262915 1 GGATAGT 70 1.0714882E-4 12.236074 6 GGACCTG 320 0.0 12.193588 6 CCTAACC 55 0.0029923585 12.130177 3 AATGCCG 55 0.003105851 12.070047 18 TCCTACA 650 0.0 12.023539 2 ATTATAC 80 2.7683554E-5 11.913567 3 GGACGTG 400 0.0 11.896183 6 TGGATAG 80 2.810053E-5 11.896183 5 GACGTGG 235 0.0 11.744316 7 CCTGGAC 155 2.3646862E-11 11.682982 3 CTGGACA 155 2.3646862E-11 11.66903 4 TAGGACC 245 0.0 11.656496 4 TAGGACG 435 0.0 11.598437 4 >>END_MODULE