FastQCFastQC Report
Thu 26 May 2016
SRR1926981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756711
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18900.24976510186848086No Hit
CTGTAGGACGTGGAATATGGCAAGA12260.16201693909563888No Hit
GTCCTACAGTGGACATTTCTAAATT11790.15580584926081423No Hit
GGTATCAACGCAGAGTACTTTTTTT11670.1542200390902207No Hit
TATCAACGCAGAGTACTTTTTTTTT9210.12171093059305335No Hit
CACACACACACACACACACACACAC8790.116160594995976No Hit
GTCCTACAGTGTGCATTTCTCATTT8680.11470693567293194No Hit
GTCCTAAAGTGTGTATTTCTCATTT8020.10598497973466753No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG7800.1030776610885794No Hit
CTTTAGGACGTGAAATATGGCGAGG7570.10003819159494179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA456.633605E-414.8136336
TAGGACC3400.014.0044854
GGTATCA3650.013.3238551
GGACCTG4250.012.5480196
TTAGGAC3600.012.434523
GGCCTCG853.9926745E-612.27833815
AGGACCT4900.012.2439215
CTACACT1405.0931703E-1112.2439214
CCTCGAA701.1026125E-412.1986117
GCCTCGA701.1026125E-412.1986116
TCCAACG701.1026125E-412.1986118
GGACGTG6100.012.0208996
GTAGGAC8650.012.0017363
TAGGACA2550.011.9504944
AGGACGT6300.011.9416035
GACGTGG3400.011.7217827
TGTAGGA9450.011.5010932
GTCCATA1001.840106E-611.4428411
CTGTAGG9100.011.4218831
CGTGGAA3450.011.2753659
Produced by FastQC (version 0.11.5)