FastQCFastQC Report
Thu 26 May 2016
SRR1926980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821687
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23380.28453656927759596No Hit
GTCCTACAGTGTGCATTTCTCATTT18780.2285541818234924No Hit
CTGTAGGACGTGGAATATGGCAAGA18660.22709377171599407No Hit
GTCCTAAAGTGTGTATTTCTCATTT16190.19703366366998626No Hit
CTTTAGGACGTGAAATATGGCGAGG13520.1645395387781479No Hit
GTATCAACGCAGAGTACTTTTTTTT12330.15005713854545588No Hit
CTGTAGGACCTGGAATATGGCGAGA12180.14823162591108294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.013.7742154
CCAACGA604.1536975E-412.64288519
AATGTCC1750.012.4972678
GGACCTG6650.012.1644886
AAATGTC1800.012.1605137
AGGACCT7300.012.12435
CTCTTAG1156.6298526E-811.6170491
GTGTAGG1406.2937033E-1011.5874131
TGTAGGA11600.011.4269372
GTATAGG1354.3910404E-911.3097191
GTAGGAC11400.011.2928093
CTGTAGG12000.011.1330041
GTAGGTC600.005707937811.1255073
TCAATAC600.005707937811.1255073
TTAGACT600.005778563711.1071484
GGACTGC600.005794359411.1030766
CCTACAC1303.0784577E-811.0032493
ATGCCGG951.386497E-510.97934919
ACTAGGT700.001443614110.8984573
TTATACT1053.3712404E-610.8804734
Produced by FastQC (version 0.11.5)