##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821687 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.304299569057317 31.0 28.0 31.0 16.0 34.0 2 28.377754546439217 31.0 28.0 31.0 16.0 34.0 3 28.173317820532635 31.0 28.0 33.0 16.0 34.0 4 31.687165550872777 35.0 32.0 37.0 19.0 37.0 5 31.972409202044087 35.0 33.0 37.0 19.0 37.0 6 31.160859305307252 35.0 32.0 37.0 16.0 37.0 7 31.214072998599224 35.0 32.0 37.0 17.0 37.0 8 31.020793805913932 35.0 32.0 37.0 11.0 37.0 9 33.215994654899006 37.0 33.0 39.0 17.0 39.0 10 33.38590120082221 37.0 34.0 39.0 17.0 39.0 11 33.55755050280703 38.0 34.0 39.0 19.0 39.0 12 33.42914759513051 38.0 34.0 39.0 17.0 39.0 13 33.5327259649964 38.0 34.0 39.0 18.0 39.0 14 34.571561920780056 39.0 34.0 41.0 18.0 41.0 15 34.161110009042375 39.0 34.0 41.0 16.0 41.0 16 34.243904309061726 39.0 34.0 41.0 17.0 41.0 17 34.470031776089925 39.0 34.0 41.0 17.0 41.0 18 34.52215381282654 39.0 34.0 41.0 17.0 41.0 19 33.938898875119115 39.0 34.0 41.0 10.0 41.0 20 34.20702530282212 39.0 34.0 41.0 11.0 41.0 21 34.183939870047844 39.0 34.0 41.0 11.0 41.0 22 34.206007883780565 39.0 34.0 41.0 10.0 41.0 23 34.20176052438459 39.0 34.0 41.0 2.0 41.0 24 32.968060830949014 39.0 32.0 41.0 2.0 41.0 25 33.51001537081638 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.26371977732182117 1101 2 -0.31778715212497843 1101 3 -1.0486884276951152 1101 4 -0.6541196935455353 1101 5 -0.449616881094947 1101 6 -2.18135168641124 1101 7 -0.6900544114334721 1101 8 -0.024636659490877122 1101 9 -0.2456301112545347 1101 10 -9.511209513959688 1101 11 -4.2095287240205295 1101 12 -1.452796513324472 1101 13 -0.8168202365756372 1101 14 -0.3301116278569012 1101 15 -1.772903466624058 1101 16 -0.250518833324719 1101 17 -0.23671424152372822 1101 18 -0.16434370934683074 1101 19 -0.9171837919245291 1101 20 -0.3255639906141923 1101 21 -0.3268707513643747 1101 22 0.0700700146983877 1101 23 -0.2203824071563929 1101 24 -0.26901808013787587 1101 25 -0.2728219862007677 1102 1 0.06057033756871988 1102 2 -0.058987729147553125 1102 3 -0.16992553120624976 1102 4 -0.26853086667629356 1102 5 0.2538147585195034 1102 6 -0.4758970412766992 1102 7 0.07439017007096638 1102 8 0.5772465448347361 1102 9 0.6297405503683251 1102 10 0.5064924696410813 1102 11 0.37158714992539643 1102 12 0.5764034332221328 1102 13 0.3332289336974199 1102 14 -0.14646425581097589 1102 15 0.4473361827764748 1102 16 0.13742399895154023 1102 17 0.14301389997700653 1102 18 0.07613627475420515 1102 19 0.4416358474020967 1102 20 0.2294878694612592 1102 21 0.10804117045660178 1102 22 -0.018066414820225418 1102 23 -0.46004825488959256 1102 24 -3.520963132786047 1102 25 -2.037829198982987 1103 1 -0.15859926495697962 1103 2 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0.7018183404486891 1107 11 0.41208958384535777 1107 12 0.48283573804999236 1107 13 0.30736894644432056 1107 14 0.4219065287043051 1107 15 -0.874718470569718 1107 16 -0.034773364312826516 1107 17 0.1307572095011622 1107 18 3.396921388230112E-4 1107 19 0.8761548892465143 1107 20 0.9831855936240359 1107 21 0.7053075794580863 1107 22 0.7016659529504281 1107 23 0.5335466231241099 1107 24 -3.3249642887165898 1107 25 -1.202165310860174 1108 1 0.21452299238782047 1108 2 -0.0320624999141188 1108 3 0.591862306075523 1108 4 0.5720879010044122 1108 5 0.4018874203191203 1108 6 -2.8038011531504736 1108 7 -1.1916365297724845 1108 8 0.3966861327522544 1108 9 0.18421339556757488 1108 10 0.5187161064273909 1108 11 0.2735243729021235 1108 12 0.21821262419665288 1108 13 0.2227164901485068 1108 14 0.04201750049805497 1108 15 -0.8933105971368818 1108 16 -0.045994948780204936 1108 17 0.04247187937417607 1108 18 -0.1348267471668123 1108 19 -0.05305309768298372 1108 20 -1.2453600434342178 1108 21 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3 -2.9512731597430175 2212 4 -1.6337377635506236 2212 5 -0.5203802786997933 2212 6 0.30885037582577723 2212 7 0.36624558394267837 2212 8 0.07587658783884876 2212 9 -0.04725770639024773 2212 10 0.18310777461478267 2212 11 0.05176502915836778 2212 12 -0.15736025309420398 2212 13 -0.19772373682994981 2212 14 -0.43312175408800613 2212 15 -1.31463371122571 2212 16 -0.4432593891114678 2212 17 -0.18665644340101295 2212 18 -0.24470621917251378 2212 19 0.4090288126900745 2212 20 -0.7361618543955757 2212 21 -0.8142707606120752 2212 22 -0.3003835849686993 2212 23 -0.10381515326079693 2212 24 1.1222243483080518 2212 25 0.6150773988272036 2213 1 -0.4812284709272774 2213 2 -0.5489170930540155 2213 3 0.06279320563973911 2213 4 -6.998711435759255 2213 5 -1.753095841810481 2213 6 -2.972134186828839 2213 7 -0.9786001117052905 2213 8 -2.656826536945207 2213 9 -0.8376608463766999 2213 10 -0.2238300475724344 2213 11 -0.21336757722387745 2213 12 -0.10349124249398045 2213 13 -0.11135199670805918 2213 14 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25 -0.9874016452248711 2215 1 0.4904141545898888 2215 2 0.06140876918980709 2215 3 0.47399229860318215 2215 4 0.9319668393110199 2215 5 0.6515828857378949 2215 6 1.411258306715549 2215 7 0.7049933965342312 2215 8 -0.21490106060798908 2215 9 0.3660021458557594 2215 10 0.16134375342561924 2215 11 0.06623320187059534 2215 12 -0.19769250163130891 2215 13 0.1911004865905639 2215 14 0.28942221183197603 2215 15 0.22832501109021308 2215 16 0.31126785352657294 2215 17 -0.035511864610754174 2215 18 -0.7183836783738826 2215 19 0.36459175858487214 2215 20 -3.5213722019262796 2215 21 -1.7438223517785048 2215 22 -0.9661839982998117 2215 23 -0.745647112782855 2215 24 -0.7772459227326465 2215 25 -0.6244867823100151 2216 1 -1.0810205011736507 2216 2 -1.6446703091947512 2216 3 -0.8566395192567313 2216 4 -0.9748291435280407 2216 5 -1.142059960545133 2216 6 -2.0842838213258865 2216 7 -10.393817689039178 2216 8 -11.026706707988659 2216 9 -6.268047250029916 2216 10 -4.047505925933464 2216 11 -2.902501027939973 2216 12 -2.871649050565388 2216 13 -2.358406579692396 2216 14 -2.666653276087473 2216 15 -3.5886662583843503 2216 16 -2.677645780682788 2216 17 -2.903872537478776 2216 18 -2.5877863289790355 2216 19 -1.980531671700927 2216 20 -2.5816826063017935 2216 21 -5.794317662631073 2216 22 -4.1728179892577835 2216 23 -3.9002114358415128 2216 24 -4.105887384829465 2216 25 -3.345158006143567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47344.0 3 7.0 4 284.0 5 2070.0 6 2842.0 7 2256.0 8 1765.0 9 1656.0 10 1550.0 11 1465.0 12 1528.0 13 1416.0 14 1413.0 15 1514.0 16 1677.0 17 1743.0 18 1926.0 19 2477.0 20 3193.0 21 4087.0 22 5184.0 23 6218.0 24 7189.0 25 8261.0 26 9371.0 27 10191.0 28 11567.0 29 13593.0 30 16519.0 31 19798.0 32 25458.0 33 33312.0 34 45538.0 35 64519.0 36 94689.0 37 152728.0 38 215339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96636951564831 19.397613438529753 13.319091881762605 25.316925164059327 2 13.335673138910115 20.496331257974532 43.689410308062214 22.47858529505314 3 18.81483377205811 24.7225065808716 29.962342790289558 26.500316856780735 4 11.670115195896352 17.075030172093584 39.58956209970979 31.665292532300278 5 12.118888243736237 38.41275405928831 37.05355132189344 12.414806375082014 6 30.275575551084156 37.94857562290251 19.635868764736458 12.139980061276882 7 27.09604100470812 31.30429687643047 22.921109392680624 18.678552726180783 8 23.293010424550182 38.8226605795757 21.96198823270916 15.92234076316496 9 25.601590201708763 16.117468482625732 21.164995599679134 37.11594571598637 10 13.989301088883261 30.116192999201356 35.42328145392212 20.47122445799326 11 33.23291376210664 23.143972452172896 25.590071486562643 18.033042299157813 12 22.929348626544506 25.313404930490385 32.81491733218427 18.942329110780836 13 28.619247065639836 22.989462716615606 26.003245049959894 22.38804516778466 14 19.394991369887357 23.85234108542229 28.39453125950956 28.358136285180795 15 21.8886972482871 33.21652317965434 23.501602437311412 21.393177134747145 16 20.287247678391665 29.27545370112267 30.06825145066286 20.369047169822803 17 20.268668125725775 30.015191093734405 29.187958136851304 20.528182643688513 18 19.9795512223622 28.617699147359005 31.737598364097792 19.66515126618101 19 22.92167028408171 27.654052580131932 27.978098306854992 21.446178828931366 20 22.513407648506593 28.64392255576681 29.288682557032715 19.553987238693875 21 21.5660975853196 27.869852158946784 27.82019041869229 22.743859837041327 22 21.715907763019516 30.002702255287655 28.283605344135864 19.99778463755697 23 20.795756226469294 29.35925884900546 28.852036375897256 20.992948548627993 24 22.40527483656113 27.834938610766578 29.65257601078922 20.107210541883074 25 21.284802777162415 28.645816850423223 29.375893120144898 20.693487252269467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 240.0 2 321.5 3 403.0 4 403.0 5 403.0 6 618.5 7 834.0 8 834.0 9 834.0 10 1335.5 11 1837.0 12 1837.0 13 1837.0 14 3061.5 15 4286.0 16 4286.0 17 4286.0 18 7547.5 19 10809.0 20 10809.0 21 10809.0 22 17648.0 23 24487.0 24 24487.0 25 24487.0 26 35121.0 27 45755.0 28 45755.0 29 45755.0 30 60367.0 31 74979.0 32 74979.0 33 74979.0 34 84271.0 35 93563.0 36 93563.0 37 93563.0 38 98917.5 39 104272.0 40 104272.0 41 104272.0 42 106761.5 43 109251.0 44 109251.0 45 109251.0 46 109035.5 47 108820.0 48 108820.0 49 108820.0 50 100460.0 51 92100.0 52 92100.0 53 92100.0 54 78013.5 55 63927.0 56 63927.0 57 63927.0 58 52870.5 59 41814.0 60 41814.0 61 41814.0 62 33013.5 63 24213.0 64 24213.0 65 24213.0 66 18138.5 67 12064.0 68 12064.0 69 12064.0 70 8661.0 71 5258.0 72 5258.0 73 5258.0 74 3559.5 75 1861.0 76 1861.0 77 1861.0 78 1246.5 79 632.0 80 632.0 81 632.0 82 417.5 83 203.0 84 203.0 85 203.0 86 129.0 87 55.0 88 55.0 89 55.0 90 35.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 4.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2451054963751404 2 0.13460113157443163 3 0.13715684926255375 4 0.06863927505242264 5 0.021419348243309193 6 0.020689143189560017 7 0.2998708754063287 8 0.3002359779332033 9 0.018985331397478603 10 0.0359017484760012 11 0.016307912867064952 12 0.017768322974563308 13 0.015577707813315776 14 0.017646622132271778 15 0.015334306128732716 16 0.0202023398203939 17 0.018620228870604015 18 0.014847502759566597 19 0.02409676677372284 20 0.01715981876310566 21 0.015699408655607306 22 0.018376827186020954 23 0.018741929712895542 24 0.015334306128732716 25 0.015699408655607306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 821687.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.431732758365 #Duplication Level Percentage of deduplicated Percentage of total 1 93.89822661988788 79.27989976454768 2 4.599732968588347 7.767268495273845 3 0.6956142472118295 1.7619574867050132 4 0.2569151571816163 0.8676716757092626 5 0.13762133110720026 0.5809803724946797 6 0.08054207186474743 0.408018401249363 7 0.0537647184979221 0.3177613840831697 8 0.043985979436224655 0.2971049968699407 9 0.030063226726908757 0.22844612923744465 >10 0.16846490845988554 2.7125150209096716 >50 0.017896376881133408 1.064755541830436 >100 0.01471919505326556 2.5861740207231865 >500 0.0014430582959350541 0.725800358149704 >1k 0.001010140807154538 1.4016463522165847 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2338 0.28453656927759596 No Hit GTCCTACAGTGTGCATTTCTCATTT 1878 0.2285541818234924 No Hit CTGTAGGACGTGGAATATGGCAAGA 1866 0.22709377171599407 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1619 0.19703366366998626 No Hit CTTTAGGACGTGAAATATGGCGAGG 1352 0.1645395387781479 No Hit GTATCAACGCAGAGTACTTTTTTTT 1233 0.15005713854545588 No Hit CTGTAGGACCTGGAATATGGCGAGA 1218 0.14823162591108294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.217008422915295E-4 0.0 5 0.0 0.0 0.0 2.43401684583059E-4 0.0 6 0.0 0.0 0.0 2.43401684583059E-4 0.0 7 0.0 0.0 0.0 3.651025268745885E-4 0.0 8 0.0 0.0 0.0 4.86803369166118E-4 0.0 9 0.0 0.0 0.0 7.30205053749177E-4 0.0 10 0.0 0.0 0.0 7.30205053749177E-4 0.0 11 0.0 0.0 0.0 7.30205053749177E-4 0.0 12 0.0 0.0 0.0 7.30205053749177E-4 0.0 13 0.0 0.0 0.0 7.30205053749177E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 470 0.0 13.774215 4 CCAACGA 60 4.1536975E-4 12.642885 19 AATGTCC 175 0.0 12.497267 8 GGACCTG 665 0.0 12.164488 6 AAATGTC 180 0.0 12.160513 7 AGGACCT 730 0.0 12.1243 5 CTCTTAG 115 6.6298526E-8 11.617049 1 GTGTAGG 140 6.2937033E-10 11.587413 1 TGTAGGA 1160 0.0 11.426937 2 GTATAGG 135 4.3910404E-9 11.309719 1 GTAGGAC 1140 0.0 11.292809 3 CTGTAGG 1200 0.0 11.133004 1 GTAGGTC 60 0.0057079378 11.125507 3 TCAATAC 60 0.0057079378 11.125507 3 TTAGACT 60 0.0057785637 11.107148 4 GGACTGC 60 0.0057943594 11.103076 6 CCTACAC 130 3.0784577E-8 11.003249 3 ATGCCGG 95 1.386497E-5 10.979349 19 ACTAGGT 70 0.0014436141 10.898457 3 TTATACT 105 3.3712404E-6 10.880473 4 >>END_MODULE