FastQCFastQC Report
Thu 26 May 2016
SRR1926980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821687
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23240.28283275748551456No Hit
CTGTAGGACGTGGAATATGGCAAGA22950.2793034330590602No Hit
GTATCAACGCAGAGTACTTTTTTTT19440.23658643741473334No Hit
GTCCTACAGTGTGCATTTCTCATTT18460.22465975487016343No Hit
CTTTAGGACGTGAAATATGGCGAGG17430.21212456811413594No Hit
GTCCTAAAGTGTGTATTTCTCATTT17070.20774333779164086No Hit
CTGTAGGACCTGGAATATGGCGAGA15200.18498528028312483No Hit
GGTATCAACGCAGAGTACTTTTTTT11630.1415380795850488No Hit
TATCAACGCAGAGTACTTTTTTTTT9610.11695450944215985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA500.001511437513.28483319
TAGGACC5250.013.2363514
TAATCAG655.343659E-513.1805595
CTAGGAC1550.012.8978943
TCCAACG905.4697193E-712.65222318
GCCGGTT604.1300355E-412.65145211
GGCGAGG5050.012.58958719
GACGTGA5400.012.4802957
CTTAGAC853.8459693E-612.3198383
ATAGGAC1251.3606041E-912.1854393
TTAGGAC6900.012.1412883
TAAGCCT1103.6976417E-812.1154644
AGGACGT10550.012.0006275
GGACGTG10300.011.9222216
GTCCTAC11850.011.9043371
ACTGTTC2000.011.862188
TGGCGAG11900.011.80165318
TGTAGGA13500.011.7202312
TGCCGGT658.0975867E-411.67826410
TAGGACG11500.011.5059284
Produced by FastQC (version 0.11.5)