FastQCFastQC Report
Thu 26 May 2016
SRR1926979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679090
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17130.25224933366711333No Hit
CTGTAGGACGTGGAATATGGCAAGA14240.20969238245298855No Hit
GTCCTAAAGTGTGTATTTCTCATTT13510.19894270273454182No Hit
GTCCTACAGTGTGCATTTCTCATTT11630.17125859606237762No Hit
GTATCAACGCAGAGTACTTTTTTTT10790.15888910159183614No Hit
CTTTAGGACGTGAAATATGGCGAGG10350.15240984258345727No Hit
CTGTAGGACCTGGAATATGGCGAGA7520.11073642668865688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG250.005892250719.0754131
GGCGTGC402.7341346E-416.6441338
CTTAGGC751.4232464E-512.7112933
TAGGACC2700.011.9926774
GTATAGT657.751176E-411.7387161
CCAACGA658.1401685E-411.67041719
GGCGAGG3250.011.67041719
AACAGGT907.282142E-611.6339277
GATATAC3450.011.6111221
TCCAACG907.600025E-611.58937418
GTCCTAG1251.7198545E-811.4452481
GTCCTAC9000.011.33927251
CCCCTAG600.005699262511.1273241
GGACAGC951.3277322E-511.0216156
GGACCTG4150.011.0095436
TCCTACA9700.011.0077172
ATCCCGT700.001511136910.83841210
GTCCTAA4950.010.79013251
TAGAGTG1158.5673673E-710.7602345
CTAGACA803.679412E-410.71404654
Produced by FastQC (version 0.11.5)