##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926979_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679090 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.349385206673638 31.0 28.0 31.0 19.0 34.0 2 28.412083818050625 31.0 28.0 31.0 20.0 34.0 3 28.194595708963465 31.0 28.0 33.0 16.0 34.0 4 31.73724101370952 35.0 32.0 37.0 19.0 37.0 5 31.995530783843083 35.0 33.0 37.0 19.0 37.0 6 31.166983757675712 35.0 32.0 37.0 17.0 37.0 7 31.218595473354046 35.0 32.0 37.0 17.0 37.0 8 31.033541945839286 35.0 32.0 37.0 11.0 37.0 9 33.23355372631021 37.0 33.0 39.0 17.0 39.0 10 33.40424096953276 37.0 34.0 39.0 17.0 39.0 11 33.57421696682325 38.0 34.0 39.0 19.0 39.0 12 33.44348613585828 38.0 34.0 39.0 18.0 39.0 13 33.55500890898114 38.0 34.0 39.0 19.0 39.0 14 34.576350704619415 39.0 34.0 41.0 19.0 41.0 15 34.161535289873214 39.0 33.0 41.0 17.0 41.0 16 34.252621891060095 39.0 34.0 41.0 17.0 41.0 17 34.493701865732085 39.0 34.0 41.0 18.0 41.0 18 34.53542682118718 39.0 34.0 41.0 18.0 41.0 19 33.91281715236567 39.0 33.0 41.0 10.0 41.0 20 34.18603719683694 39.0 34.0 41.0 16.0 41.0 21 34.17274440795771 39.0 34.0 41.0 11.0 41.0 22 34.204952215464814 39.0 34.0 41.0 11.0 41.0 23 34.207283276148964 39.0 34.0 41.0 8.0 41.0 24 32.91393335198575 39.0 32.0 41.0 2.0 41.0 25 33.49012207512995 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.40594252554457455 1101 2 -0.6099229242696325 1101 3 -1.4763283318486415 1101 4 -1.0932852543358216 1101 5 -0.664762901354802 1101 6 -3.140034609709833 1101 7 -0.9857883285503277 1101 8 -0.5062464967249802 1101 9 -0.4701466537743997 1101 10 -9.12438535074779 1101 11 -4.196754680942739 1101 12 -1.914205041755693 1101 13 -1.2000146375486267 1101 14 -0.9614686852575076 1101 15 -2.746542821439103 1101 16 -1.0761433950861132 1101 17 -0.7726181891628485 1101 18 -0.7161989037177534 1101 19 -1.2072303766918466 1101 20 -0.5991360487733104 1101 21 -0.767331302455716 1101 22 -0.4558437698567914 1101 23 -0.5145472252849714 1101 24 -0.7789398308449123 1101 25 -0.6126531406721512 1102 1 -0.01807375205083872 1102 2 -0.32691178345102756 1102 3 -0.380645132300657 1102 4 -0.27279135114702413 1102 5 0.11291460939426656 1102 6 -0.7129234891140008 1102 7 0.12114645757095133 1102 8 0.42351441722253824 1102 9 0.42739196004070124 1102 10 0.4785592479927061 1102 11 0.10645687876170484 1102 12 0.25575875032978246 1102 13 0.06943708133649551 1102 14 -0.07435953612825585 1102 15 0.5075924313303517 1102 16 0.486718442706902 1102 17 0.18044159810168026 1102 18 0.013075037660584599 1102 19 0.5460822460802177 1102 20 0.3882770517237262 1102 21 0.16434112109660504 1102 22 0.2695878441341151 1102 23 -0.04511562755357801 1102 24 -3.184794680093777 1102 25 -1.1847249792883048 1103 1 0.22966510871723855 1103 2 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-1.4335442416167723 2214 25 -0.3902874495742523 2215 1 0.4328746072849192 2215 2 -0.13954501028918997 2215 3 0.22130716407695417 2215 4 0.6152782556148182 2215 5 0.7045315236578844 2215 6 1.3742851810081511 2215 7 0.8554893374972181 2215 8 -0.27603371186272696 2215 9 0.16732367885893495 2215 10 -0.15103087505495694 2215 11 -0.11146986567923278 2215 12 -0.3921866868613648 2215 13 -0.09455857600795525 2215 14 -0.08558122731297146 2215 15 0.03385117144243566 2215 16 0.3060201312680775 2215 17 -0.141493127435929 2215 18 -0.6156391324106423 2215 19 0.22343726507502026 2215 20 -3.4489726112717065 2215 21 -1.9947794048549028 2215 22 -0.9770684319179352 2215 23 -1.053749158170504 2215 24 -0.6447459374099225 2215 25 -0.592263180331166 2216 1 0.04240162895580113 2216 2 -0.7943955695980662 2216 3 -0.00875881029011083 2216 4 -0.005269671150795574 2216 5 -0.03479045548338533 2216 6 -1.2144272344673368 2216 7 -9.570453650239717 2216 8 -10.251963014143787 2216 9 -5.068533561644632 2216 10 -2.954859213072254 2216 11 -1.8547277221030924 2216 12 -1.286031671777451 2216 13 -1.1656631194297375 2216 14 -1.1433734865798186 2216 15 -2.2341698817244335 2216 16 -1.3342064340713762 2216 17 -1.5634119928742436 2216 18 -1.2207344594631238 2216 19 -0.388598009000539 2216 20 -1.4043946692352236 2216 21 -3.6879297967973876 2216 22 -2.5747324816462474 2216 23 -2.557454701004488 2216 24 -2.671812478639044 2216 25 -2.0417877790033003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37790.0 3 7.0 4 231.0 5 1708.0 6 2282.0 7 1847.0 8 1482.0 9 1311.0 10 1289.0 11 1215.0 12 1151.0 13 1150.0 14 1267.0 15 1245.0 16 1305.0 17 1413.0 18 1674.0 19 2138.0 20 2685.0 21 3448.0 22 4326.0 23 5114.0 24 6003.0 25 6938.0 26 8143.0 27 8625.0 28 9898.0 29 11576.0 30 13936.0 31 16913.0 32 21888.0 33 29148.0 34 38799.0 35 54611.0 36 78709.0 37 125435.0 38 172390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.620700850790655 18.975509330657243 12.935854959747935 25.46793485880417 2 13.72627369681349 20.379149015855134 43.12086499175026 22.773712295581117 3 18.852995647198988 24.673245019050878 30.062637868189164 26.411121465560967 4 11.86460158344004 16.961345260952275 39.06850227437158 32.105550881236105 5 12.350743136062167 38.22006725119928 36.906120801770975 12.523068810967573 6 30.668862184609086 37.60521622071436 19.417961909334732 12.307959685341824 7 26.920776418567765 31.348867021410143 22.978132028964318 18.752224531057777 8 23.635995800224165 38.28086813087825 21.79261317908559 16.290522889812 9 25.827508299384654 15.687383462500332 20.693377871646 37.79173036646901 10 14.22363313618184 29.6482945045556 35.46222572495525 20.665846634307307 11 33.874440703527945 22.68571293915267 25.18461570183423 18.255230655485157 12 22.882058638561475 24.894657077674665 32.816621574053755 19.40666270971011 13 28.824203435162847 22.6337230366816 25.951650863503676 22.590422664651875 14 19.897374568098336 23.14374335394286 28.02610998359289 28.93277209436591 15 22.400626821663643 32.47233722298562 23.305659510354488 21.82137644499625 16 20.982292113119566 28.533513608252587 29.502933149523614 20.981261129104237 17 20.636465930647688 29.389013915236063 28.844069234485275 21.13045091963097 18 20.522046239811953 28.328586030262954 30.860464636765037 20.288903093160055 19 23.35006539644385 27.086647342312087 27.552817938656958 22.010469322587106 20 22.871521758661267 28.332137896905756 28.589291737055046 20.207048607377928 21 22.135556275589856 27.419217060885448 27.489469498365192 22.955757165159504 22 22.18129439609528 29.140039441921843 27.844995309091818 20.833670852891057 23 21.525219781109943 28.672947740036026 28.253342189872615 21.548490288981416 24 22.921836822160397 27.472359152077313 28.74162158659277 20.864182439169515 25 22.010188797624675 27.950561208634706 28.850144554854996 21.189105438885626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 157.0 2 241.0 3 325.0 4 325.0 5 325.0 6 490.0 7 655.0 8 655.0 9 655.0 10 1028.5 11 1402.0 12 1402.0 13 1402.0 14 2396.5 15 3391.0 16 3391.0 17 3391.0 18 5904.5 19 8418.0 20 8418.0 21 8418.0 22 13576.5 23 18735.0 24 18735.0 25 18735.0 26 27354.5 27 35974.0 28 35974.0 29 35974.0 30 47592.5 31 59211.0 32 59211.0 33 59211.0 34 66165.0 35 73119.0 36 73119.0 37 73119.0 38 77394.5 39 81670.0 40 81670.0 41 81670.0 42 84233.0 43 86796.0 44 86796.0 45 86796.0 46 87372.5 47 87949.0 48 87949.0 49 87949.0 50 82758.5 51 77568.0 52 77568.0 53 77568.0 54 67659.5 55 57751.0 56 57751.0 57 57751.0 58 48798.0 59 39845.0 60 39845.0 61 39845.0 62 32053.5 63 24262.0 64 24262.0 65 24262.0 66 18539.0 67 12816.0 68 12816.0 69 12816.0 70 9266.5 71 5717.0 72 5717.0 73 5717.0 74 3954.0 75 2191.0 76 2191.0 77 2191.0 78 1498.5 79 806.0 80 806.0 81 806.0 82 523.5 83 241.0 84 241.0 85 241.0 86 154.5 87 68.0 88 68.0 89 68.0 90 41.0 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 4.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21867499153278652 2 0.13061597137345565 3 0.1334138332179829 4 0.06493984597034266 5 0.021057591777231294 6 0.019290521138582515 7 0.29171391126360274 8 0.28140599920481824 9 0.018259729932704062 10 0.037550251071286574 11 0.01649265929405528 12 0.017228938726825606 13 0.01649265929405528 14 0.017081682840271537 15 0.016050891634393084 16 0.018701497592366254 17 0.017965218159595932 18 0.01590363574783902 19 0.02267740652932601 20 0.017523450499933736 21 0.01619814752094715 22 0.017228938726825606 23 0.017523450499933736 24 0.016639915180609345 25 0.016050891634393084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 679090.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.44319974120882 #Duplication Level Percentage of deduplicated Percentage of total 1 93.24762639551265 78.7412794110989 2 5.122426885744306 8.651082333452893 3 0.8150806930193167 2.064840652974992 4 0.2817662850631222 0.9517298675969442 5 0.13807272402485568 0.5829651306821845 6 0.08376227317424777 0.42438926186584214 7 0.048788687429859 0.2883911014425691 8 0.04066584609624421 0.27471633316403254 9 0.0292046959605707 0.22195241769417712 >10 0.15734237391727518 2.499664613358853 >50 0.019028344244335103 1.1266807001266383 >100 0.01361628036162419 2.2528754196307377 >500 0.0015711092710220772 0.7747898448909843 >1k 0.0010474061806813849 1.1446429120202612 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1713 0.25224933366711333 No Hit CTGTAGGACGTGGAATATGGCAAGA 1424 0.20969238245298855 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1351 0.19894270273454182 No Hit GTCCTACAGTGTGCATTTCTCATTT 1163 0.17125859606237762 No Hit GTATCAACGCAGAGTACTTTTTTTT 1079 0.15888910159183614 No Hit CTTTAGGACGTGAAATATGGCGAGG 1035 0.15240984258345727 No Hit CTGTAGGACCTGGAATATGGCGAGA 752 0.11073642668865688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCACG 25 0.0058922507 19.075413 1 GGCGTGC 40 2.7341346E-4 16.644133 8 CTTAGGC 75 1.4232464E-5 12.711293 3 TAGGACC 270 0.0 11.992677 4 GTATAGT 65 7.751176E-4 11.738716 1 CCAACGA 65 8.1401685E-4 11.670417 19 GGCGAGG 325 0.0 11.670417 19 AACAGGT 90 7.282142E-6 11.633927 7 GATATAC 345 0.0 11.611122 1 TCCAACG 90 7.600025E-6 11.589374 18 GTCCTAG 125 1.7198545E-8 11.445248 1 GTCCTAC 900 0.0 11.3392725 1 CCCCTAG 60 0.0056992625 11.127324 1 GGACAGC 95 1.3277322E-5 11.021615 6 GGACCTG 415 0.0 11.009543 6 TCCTACA 970 0.0 11.007717 2 ATCCCGT 70 0.0015111369 10.838412 10 GTCCTAA 495 0.0 10.7901325 1 TAGAGTG 115 8.5673673E-7 10.760234 5 CTAGACA 80 3.679412E-4 10.7140465 4 >>END_MODULE