FastQCFastQC Report
Thu 26 May 2016
SRR1926979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679090
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16970.2498932394822483No Hit
CTGTAGGACGTGGAATATGGCAAGA16960.24974598359569422No Hit
GTATCAACGCAGAGTACTTTTTTTT16130.23752374501170684No Hit
GTCCTAAAGTGTGTATTTCTCATTT13990.20601098528913692No Hit
CTTTAGGACGTGAAATATGGCGAGG13580.19997349394042027No Hit
GTCCTACAGTGTGCATTTCTCATTT12010.17685431975143207No Hit
GGTATCAACGCAGAGTACTTTTTTT9820.14460528059609182No Hit
CTGTAGGACCTGGAATATGGCGAGA9580.14107113931879428No Hit
TATCAACGCAGAGTACTTTTTTTTT8790.12943792428102313No Hit
CTGAAGGACCTGGAATATGGCGAGA7390.10882210016345403No Hit
GATATACACTGTTCTACAAATCCCG7030.10352088824750769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT307.594358E-419.0451554
ATGTCGA601.4840425E-615.81253117
GTCGAGG551.1405633E-515.52503119
TGTCGAG653.411591E-614.59618418
TTAAGGT400.005192601614.2838674
GATCAAA400.00531865714.23232711
TAGGACC3200.013.9862864
CGGTTTC551.978309E-413.80104413
TATGTCG707.3472347E-613.55359816
GTCCTAA3800.013.5471021
AGTAGTG751.4400839E-512.6967715
GTAATAC604.0056987E-412.696773
TTTATAC604.0056987E-412.696773
TCCTAGA853.783758E-612.3370122
CCAACGA658.102071E-411.67694819
GTATAAG907.159937E-611.6516231
CTATCCT752.0253655E-411.42709354
AATGTCC1501.7826096E-1011.3858618
TGTAGGA9550.011.2800582
AACAGTG2700.011.2469527
Produced by FastQC (version 0.11.5)