##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926979_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679090 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.82136830169786 31.0 30.0 33.0 25.0 34.0 2 30.074951773697155 31.0 30.0 33.0 26.0 34.0 3 30.168690453400874 31.0 30.0 34.0 26.0 34.0 4 33.766296072685506 35.0 35.0 37.0 28.0 37.0 5 33.73720567229675 37.0 35.0 37.0 28.0 37.0 6 33.72541047578377 37.0 35.0 37.0 29.0 37.0 7 33.71427793076028 37.0 35.0 37.0 29.0 37.0 8 33.74788172407192 37.0 35.0 37.0 29.0 37.0 9 35.27866556715605 39.0 35.0 39.0 30.0 39.0 10 35.143774757395924 39.0 35.0 39.0 29.0 39.0 11 35.19985863434891 39.0 35.0 39.0 29.0 39.0 12 35.087997172686975 39.0 35.0 39.0 29.0 39.0 13 35.111273910674576 39.0 35.0 39.0 29.0 39.0 14 36.191182317513146 40.0 36.0 41.0 29.0 41.0 15 36.19216745939419 40.0 36.0 41.0 29.0 41.0 16 36.17441870738783 40.0 36.0 41.0 29.0 41.0 17 36.14591733054529 40.0 36.0 41.0 29.0 41.0 18 36.11982358744791 40.0 36.0 41.0 29.0 41.0 19 36.19109101886348 40.0 37.0 41.0 29.0 41.0 20 36.147768337039274 40.0 37.0 41.0 28.0 41.0 21 36.09175808802957 40.0 36.0 41.0 28.0 41.0 22 36.00792089413774 40.0 36.0 41.0 28.0 41.0 23 35.93954998601069 40.0 36.0 41.0 28.0 41.0 24 35.866285764773444 40.0 36.0 41.0 27.0 41.0 25 35.7517457185351 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11494534232770093 1101 2 -0.08037145405508994 1101 3 0.17163317301129055 1101 4 0.19284144195092523 1101 5 0.010222268018985403 1101 6 -0.11208360130930828 1101 7 0.015618220110724224 1101 8 -0.02427259341847332 1101 9 0.2211867455168246 1101 10 0.18001884661477874 1101 11 0.09990586061261553 1101 12 0.1255451681151385 1101 13 0.28788628918496784 1101 14 0.10824731785687902 1101 15 0.10523746017719304 1101 16 0.26101937601769976 1101 17 0.23816610727465815 1101 18 0.1692689933367646 1101 19 0.1711010396042525 1101 20 0.18543996173526978 1101 21 0.23932752542908275 1101 22 -0.06571034600285941 1101 23 -1.4588625469968974E-4 1101 24 0.2947095014590744 1101 25 0.14934565976989234 1102 1 -0.23461031495581963 1102 2 -0.24577241885424783 1102 3 0.021015158887866647 1102 4 -0.06312808771202327 1102 5 -0.15774973309072493 1102 6 -0.22576405503116348 1102 7 -0.26489708466885986 1102 8 -0.14271098864468001 1102 9 -0.189142519888307 1102 10 -0.055037894022532896 1102 11 -0.06228384934806286 1102 12 -0.18153107581422034 1102 13 -0.11152470976934126 1102 14 -0.05210913143019269 1102 15 0.05731253037258455 1102 16 -0.28692130519513626 1102 17 -0.2029576693236308 1102 18 -0.021502917126134946 1102 19 0.045720846339555976 1102 20 0.07425432783254848 1102 21 -0.055506317538522865 1102 22 -0.11327146908842423 1102 23 -0.20555847660934745 1102 24 -0.15569134647480354 1102 25 -0.12431524188136223 1103 1 -0.47135534312115723 1103 2 -0.31087432168434503 1103 3 -0.09238911698242092 1103 4 -0.08069417504204068 1103 5 -0.14096150642808425 1103 6 -0.30237048630506536 1103 7 -0.18535081274850285 1103 8 -0.28714132130598813 1103 9 -0.24787459672491252 1103 10 -0.21927233321273576 1103 11 -0.1473450198177204 1103 12 -0.21613097593567687 1103 13 -0.13791545682384765 1103 14 -0.3603115026207817 1103 15 -0.12400401678920048 1103 16 -0.06595749780864679 1103 17 -0.07829239602428828 1103 18 -0.16848056365063258 1103 19 -0.0037724652917745516 1103 20 -0.21356028443936026 1103 21 -0.15894945561547047 1103 22 -0.017066442690484962 1103 23 -0.12572037849409412 1103 24 -0.07587893728515382 1103 25 -0.04661137970553142 1104 1 -0.6443319052099596 1104 2 -0.44052576929622234 1104 3 -0.17634088889498756 1104 4 -0.2529460202360312 1104 5 -0.25086138213306697 1104 6 -0.1499066512975915 1104 7 0.012059640179316489 1104 8 -0.2094528847915953 1104 9 -0.3551630623899342 1104 10 -0.17477107204338438 1104 11 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0.5311612177426355 1107 9 0.5864439958670928 1107 10 0.7779790696964781 1107 11 0.6990808173895644 1107 12 0.6868887318803658 1107 13 0.7171448820842912 1107 14 0.6311487068487551 1107 15 0.5105253081569359 1107 16 0.5519085805964181 1107 17 0.625137332233578 1107 18 0.36732815135556507 1107 19 0.4385385661917951 1107 20 0.4868939345486538 1107 21 0.5474044800282911 1107 22 0.744525809935773 1107 23 0.5662946805819971 1107 24 0.5189229979797645 1107 25 0.4279645904920173 1108 1 0.455142385644951 1108 2 0.34939860730269245 1108 3 0.3934997115941776 1108 4 0.5086772121723868 1108 5 0.47403530336917044 1108 6 0.6828250683616801 1108 7 0.2968449990390596 1108 8 0.3313247149385248 1108 9 0.23711716588591258 1108 10 0.19655107240011915 1108 11 0.30474384875545013 1108 12 0.36871222798017556 1108 13 0.44120728503636997 1108 14 0.5537387216457077 1108 15 0.613831787760887 1108 16 0.5329054567952483 1108 17 0.18224717677423286 1108 18 0.2749823776210718 1108 19 0.3616190725764099 1108 20 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7 0.36059688303677717 1110 8 0.2812796722345752 1110 9 0.3433789679405521 1110 10 0.13380918441785639 1110 11 0.41965384996814237 1110 12 0.3085091893300742 1110 13 0.4687924763812461 1110 14 0.3557240654428071 1110 15 0.4793394710067318 1110 16 0.3888900729794358 1110 17 0.5256068427652636 1110 18 0.4897849693089569 1110 19 0.5297054506232897 1110 20 0.4756981902954891 1110 21 0.4689481215347442 1110 22 0.41325884940624746 1110 23 0.3932649736312328 1110 24 0.4758414582149513 1110 25 0.42296078559015626 1111 1 0.2474107802180825 1111 2 0.060406250960785 1111 3 0.13331528085916844 1111 4 0.10092749994124262 1111 5 0.08428375459073578 1111 6 0.12176763099181898 1111 7 0.03893426558415314 1111 8 -0.01906781471137009 1111 9 -0.030462601053649507 1111 10 -0.16946762934201587 1111 11 -0.0092333108042979 1111 12 -0.16795793071302967 1111 13 -0.016786222380950733 1111 14 -0.021320448803983538 1111 15 -0.16640851539875 1111 16 -0.2692647730318356 1111 17 -0.11924404789240839 1111 18 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0.5285926238901482 2214 12 0.5473272101992137 2214 13 0.5670453833391349 2214 14 0.42505979394947957 2214 15 0.5401731988519174 2214 16 0.46051505109213764 2214 17 0.5036206431141608 2214 18 0.6673493504009258 2214 19 0.6009591552517222 2214 20 0.6345059646649744 2214 21 0.8374946101630769 2214 22 0.622597107112199 2214 23 0.6584353054751446 2214 24 0.5543698533407238 2214 25 0.5619613627664037 2215 1 0.5349993811100759 2215 2 0.47366458933914757 2215 3 0.5062853407581436 2215 4 0.6454496925972464 2215 5 0.6331814351284919 2215 6 0.44446338205987956 2215 7 0.1823765872051979 2215 8 0.2894471005868837 2215 9 0.1815029582094141 2215 10 0.2651276654050534 2215 11 0.054197184328231174 2215 12 0.1503247880652765 2215 13 0.04688987575387671 2215 14 0.18295867509725383 2215 15 0.26387777635571297 2215 16 0.12044774630744826 2215 17 0.3268273208984951 2215 18 0.12588415398053598 2215 19 0.11273599557950575 2215 20 -0.15161122589918108 2215 21 -0.13808495049955383 2215 22 -0.05575458433967384 2215 23 0.02552482263516964 2215 24 -0.15140823847281837 2215 25 -0.019710059346571995 2216 1 0.207400976699617 2216 2 0.4100242281627686 2216 3 -1.3669342412596173 2216 4 -1.0809817836521347 2216 5 0.011622429874172724 2216 6 0.25286550412816666 2216 7 0.11635061923963264 2216 8 0.4209964886301307 2216 9 0.027972334945332022 2216 10 -0.15763326688110624 2216 11 -0.0032076942750478565 2216 12 0.15785104095754576 2216 13 -0.03581440024063909 2216 14 0.023312971358208756 2216 15 0.005752878248792115 2216 16 0.08244854499962884 2216 17 -0.17910678250971301 2216 18 -0.005845463290363284 2216 19 -0.1442162907890321 2216 20 0.049102063052387734 2216 21 0.05356303265313045 2216 22 0.0549113963092438 2216 23 0.03281251484852277 2216 24 -0.15274612265647391 2216 25 -0.06516970370419983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16868.0 3 18.0 4 259.0 5 1756.0 6 2393.0 7 1737.0 8 1295.0 9 1191.0 10 1082.0 11 940.0 12 886.0 13 841.0 14 836.0 15 853.0 16 905.0 17 922.0 18 1044.0 19 1256.0 20 1654.0 21 2043.0 22 2710.0 23 3170.0 24 3930.0 25 4438.0 26 5435.0 27 6124.0 28 6905.0 29 8538.0 30 11087.0 31 14148.0 32 18396.0 33 24245.0 34 32933.0 35 45401.0 36 67513.0 37 116596.0 38 268742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.435414175754346 19.81810381557396 10.643683685218464 28.102798323453225 2 13.769851858294624 20.993252616324924 41.21564944857792 24.021246076802534 3 18.547712464505793 25.14374418248442 29.542522356933297 26.766020996076488 4 11.989574283231972 17.74212549146652 38.205245254679056 32.06305497062245 5 12.613411284800199 38.96547407794656 35.64041204320711 12.780702594046137 6 31.210836671281434 38.50989859828343 17.85914277651286 12.42012195392228 7 27.747436452598308 31.87488769291044 21.91056084319426 18.46711501129699 8 24.255214492629833 39.385968917014345 19.902477242971866 16.456339347383956 9 26.067862507731732 15.804689110777298 20.109275131807607 38.018173249683365 10 14.573295006067175 30.205253113108633 34.234475690067505 20.98697619075669 11 33.94668480950025 22.981609764143162 24.934026059706415 18.137679366650172 12 23.161867569056056 25.13496081497778 32.12111573497318 19.582055880992982 13 29.060721411899838 22.652392557632698 25.808403955322234 22.47848207514523 14 20.059638634054398 23.446671280684445 27.511522773122856 28.9821673121383 15 22.062002627130127 32.690540564420644 23.307238744867966 21.940218063581263 16 21.188154095130667 28.900420711926454 28.854623928892764 21.056801264050115 17 20.580513010813014 29.774300570322886 28.42823252916775 21.216953889696345 18 20.583518753856282 28.501060983349703 30.701368160486066 20.214052102307953 19 23.098037367763823 27.346529463044554 27.538551704639165 22.01688146455246 20 23.041083101281572 28.47911975932392 28.198007030027078 20.28179010936743 21 22.10780711301323 27.391831330659826 27.371213047249743 23.1291485090772 22 22.48788820333093 29.262984287796908 27.398724764022443 20.850402744849724 23 21.51500024297257 28.89091120604221 28.05847245353702 21.535616097448198 24 22.73442803520599 27.509501700071716 28.907698401829514 20.848371862892787 25 22.069673398580967 28.158732589749857 28.5394092369679 21.232184774701278 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 237.0 3 235.0 4 235.0 5 235.0 6 418.5 7 602.0 8 602.0 9 602.0 10 997.5 11 1393.0 12 1393.0 13 1393.0 14 2274.0 15 3155.0 16 3155.0 17 3155.0 18 5557.5 19 7960.0 20 7960.0 21 7960.0 22 12996.5 23 18033.0 24 18033.0 25 18033.0 26 26107.0 27 34181.0 28 34181.0 29 34181.0 30 45721.5 31 57262.0 32 57262.0 33 57262.0 34 64195.5 35 71129.0 36 71129.0 37 71129.0 38 75693.0 39 80257.0 40 80257.0 41 80257.0 42 83805.0 43 87353.0 44 87353.0 45 87353.0 46 87990.0 47 88627.0 48 88627.0 49 88627.0 50 84454.5 51 80282.0 52 80282.0 53 80282.0 54 69593.0 55 58904.0 56 58904.0 57 58904.0 58 50002.5 59 41101.0 60 41101.0 61 41101.0 62 33253.0 63 25405.0 64 25405.0 65 25405.0 66 19378.0 67 13351.0 68 13351.0 69 13351.0 70 9700.5 71 6050.0 72 6050.0 73 6050.0 74 4223.0 75 2396.0 76 2396.0 77 2396.0 78 1605.5 79 815.0 80 815.0 81 815.0 82 545.0 83 275.0 84 275.0 85 275.0 86 173.5 87 72.0 88 72.0 89 72.0 90 42.5 91 13.0 92 13.0 93 13.0 94 6.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11647940626426541 2 0.11088368257521095 3 0.015609123974730889 4 0.0 5 0.005153956029392275 6 0.335301653683606 7 0.02179387121000162 8 0.010160656172230486 9 0.01030791205878455 10 0.00382865305040569 11 0.00500670014283821 12 5.8902354621626E-4 13 7.362794327703251E-4 14 0.0 15 0.0032396295041894297 16 4.41767659662195E-4 17 1.47255886554065E-4 18 4.41767659662195E-4 19 2.9451177310813E-4 20 4.41767659662195E-4 21 0.011927726810879264 22 0.0 23 1.47255886554065E-4 24 1.47255886554065E-4 25 0.00530121191594634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 679090.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.69639210960217 #Duplication Level Percentage of deduplicated Percentage of total 1 91.46446415950335 74.7231672806944 2 6.504570105544712 10.627998196939544 3 1.069201436691921 2.620496994483995 4 0.3457143974745327 1.1297447589605714 5 0.16377993041039146 0.669011470724535 6 0.09397020013416985 0.46062157900673584 7 0.059587736055310925 0.3407672134788713 8 0.045022397556207724 0.2942533955573073 9 0.035833755274907626 0.2634739669528541 >10 0.17943866680106194 2.7641879131892826 >50 0.018757881904666797 1.0802264749069928 >100 0.01713428075202798 2.845295810955078 >500 0.0014428867981291051 0.859924719868835 >1k 0.0010821650985968288 1.320830224281055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1697 0.2498932394822483 No Hit CTGTAGGACGTGGAATATGGCAAGA 1696 0.24974598359569422 No Hit GTATCAACGCAGAGTACTTTTTTTT 1613 0.23752374501170684 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1399 0.20601098528913692 No Hit CTTTAGGACGTGAAATATGGCGAGG 1358 0.19997349394042027 No Hit GTCCTACAGTGTGCATTTCTCATTT 1201 0.17685431975143207 No Hit GGTATCAACGCAGAGTACTTTTTTT 982 0.14460528059609182 No Hit CTGTAGGACCTGGAATATGGCGAGA 958 0.14107113931879428 No Hit TATCAACGCAGAGTACTTTTTTTTT 879 0.12943792428102313 No Hit CTGAAGGACCTGGAATATGGCGAGA 739 0.10882210016345403 No Hit GATATACACTGTTCTACAAATCCCG 703 0.10352088824750769 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.47255886554065E-4 5 0.0 0.0 0.0 0.0 1.47255886554065E-4 6 0.0 0.0 0.0 0.0 1.47255886554065E-4 7 0.0 0.0 0.0 0.0 1.47255886554065E-4 8 0.0 0.0 0.0 0.0 1.47255886554065E-4 9 0.0 0.0 0.0 0.0 1.47255886554065E-4 10 0.0 0.0 0.0 0.0 1.47255886554065E-4 11 0.0 0.0 0.0 0.0 1.47255886554065E-4 12 0.0 0.0 0.0 0.0 1.47255886554065E-4 13 0.0 0.0 0.0 0.0 1.47255886554065E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAACGT 30 7.594358E-4 19.045155 4 ATGTCGA 60 1.4840425E-6 15.812531 17 GTCGAGG 55 1.1405633E-5 15.525031 19 TGTCGAG 65 3.411591E-6 14.596184 18 TTAAGGT 40 0.0051926016 14.283867 4 GATCAAA 40 0.005318657 14.232327 11 TAGGACC 320 0.0 13.986286 4 CGGTTTC 55 1.978309E-4 13.801044 13 TATGTCG 70 7.3472347E-6 13.553598 16 GTCCTAA 380 0.0 13.547102 1 AGTAGTG 75 1.4400839E-5 12.696771 5 GTAATAC 60 4.0056987E-4 12.69677 3 TTTATAC 60 4.0056987E-4 12.69677 3 TCCTAGA 85 3.783758E-6 12.337012 2 CCAACGA 65 8.102071E-4 11.676948 19 GTATAAG 90 7.159937E-6 11.651623 1 CTATCCT 75 2.0253655E-4 11.4270935 4 AATGTCC 150 1.7826096E-10 11.385861 8 TGTAGGA 955 0.0 11.280058 2 AACAGTG 270 0.0 11.246952 7 >>END_MODULE