FastQCFastQC Report
Thu 26 May 2016
SRR1926978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615902
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8230.13362515465122698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCG453.4298697E-516.947463
CGGTCGT350.002133684716.3248654
GGCTCGG400.00533877514.22392817
GGTCGTC500.001476318913.3254525
ACTGTTC1102.6884663E-912.9687648
CCTACCC603.9681297E-412.7105963
GTCCTAG1001.3662248E-712.3978931
GTCCTAC3850.012.1377971
ATTAGGC550.002986343812.1328423
ATTACCC550.002986343812.1328423
TTAGAAC802.760166E-511.9161833
GTGCATT3500.011.37821811
CCTAGCA855.126401E-511.2152322
CCAGGGG600.00571945511.1217723
CTCGGTC600.00571945511.1217722
GTAGGAC3000.011.1217713
CCTACAG4300.011.0848223
GTGTAGC1552.910383E-1011.075041
TAGCCCT1552.9831426E-1011.058784
TCCTACA4750.011.0381492
Produced by FastQC (version 0.11.5)