##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926978_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615902 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.248904858240433 30.0 26.0 31.0 10.0 31.0 2 26.921867439949864 31.0 27.0 31.0 10.0 33.0 3 26.749122425320913 31.0 27.0 31.0 10.0 34.0 4 30.351788109147236 35.0 30.0 37.0 10.0 37.0 5 30.10490954729811 35.0 30.0 37.0 10.0 37.0 6 29.772290072121862 35.0 30.0 37.0 8.0 37.0 7 29.8431893385636 35.0 30.0 37.0 7.0 37.0 8 29.459407503141733 35.0 29.0 37.0 2.0 37.0 9 31.545268565453593 37.0 31.0 39.0 2.0 39.0 10 31.686562147874174 37.0 32.0 39.0 2.0 39.0 11 31.868699890567004 37.0 32.0 39.0 2.0 39.0 12 31.728429198151655 37.0 32.0 39.0 2.0 39.0 13 31.830516867943278 37.0 32.0 39.0 2.0 39.0 14 32.74640121318002 38.0 32.0 40.0 2.0 41.0 15 32.28518660436238 38.0 31.0 40.0 2.0 41.0 16 32.341521865491586 38.0 32.0 40.0 2.0 41.0 17 32.57000139632604 38.0 32.0 40.0 2.0 41.0 18 32.59990225717728 38.0 32.0 40.0 2.0 41.0 19 31.9884121175122 38.0 31.0 40.0 2.0 41.0 20 32.245902757256836 38.0 32.0 40.0 2.0 41.0 21 32.25653431877149 38.0 32.0 40.0 2.0 41.0 22 32.25535718344802 38.0 32.0 40.0 2.0 41.0 23 32.21767261674747 38.0 32.0 40.0 2.0 41.0 24 30.891859419193313 38.0 29.0 40.0 2.0 41.0 25 31.457014265256486 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.44652726406951615 1101 2 -0.6004773665467589 1101 3 -1.5224818655177188 1101 4 -0.8883005654200815 1101 5 -0.7236765273868428 1101 6 -2.801333710983588 1101 7 -1.0386733631580185 1101 8 -0.5255614593816738 1101 9 -0.6654287361177111 1101 10 -8.974771230557455 1101 11 -4.52062304429262 1101 12 -2.061770809224136 1101 13 -1.2040339266423494 1101 14 -1.0297667860433855 1101 15 -2.2462726294950706 1101 16 -0.6338525904891306 1101 17 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0.9153648751202859 2215 4 1.03660119139915 2215 5 1.377099786374906 2215 6 1.7279273461900893 2215 7 1.072283641002869 2215 8 -0.8532313622942951 2215 9 0.21349135942859832 2215 10 0.10357289026239158 2215 11 0.11890153576593931 2215 12 -0.3320551365473001 2215 13 0.20775910772349704 2215 14 0.5358057212766951 2215 15 0.5488325484984529 2215 16 0.6461886331200546 2215 17 0.07718115890298805 2215 18 -0.6363179152635148 2215 19 0.37918696039728417 2215 20 -3.8594001444346517 2215 21 -2.1528083534850175 2215 22 -1.0217495561251013 2215 23 -0.6025404238904812 2215 24 -0.9837697958133127 2215 25 -0.5987045165873255 2216 1 -0.2640394840190048 2216 2 -1.3460975509903683 2216 3 -0.7265414175649383 2216 4 -0.7973591731621035 2216 5 -0.7084620100548413 2216 6 -2.445621944940804 2216 7 -9.857347558658006 2216 8 -11.197362585505637 2216 9 -6.3714169114959915 2216 10 -4.220322592757718 2216 11 -3.0363961421578516 2216 12 -2.7695227546529004 2216 13 -2.57189681992946 2216 14 -2.5353637949966767 2216 15 -3.9076255787682754 2216 16 -2.8483859276637027 2216 17 -3.061378023424737 2216 18 -2.664970004190085 2216 19 -2.0107116548351627 2216 20 -2.92094340664557 2216 21 -5.549863367379793 2216 22 -4.2322642516175435 2216 23 -3.97254478072718 2216 24 -4.055804791809731 2216 25 -3.6705481650259273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52936.0 3 1.0 4 405.0 5 2345.0 6 2710.0 7 2230.0 8 1828.0 9 1663.0 10 1533.0 11 1528.0 12 1490.0 13 1462.0 14 1501.0 15 1480.0 16 1550.0 17 1737.0 18 1962.0 19 2409.0 20 3103.0 21 4015.0 22 5085.0 23 5799.0 24 6725.0 25 7707.0 26 8423.0 27 9322.0 28 10261.0 29 12329.0 30 14439.0 31 17247.0 32 21855.0 33 28194.0 34 37258.0 35 50723.0 36 72027.0 37 109057.0 38 111563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98074184966113 18.847765419944835 12.81631125466809 25.355181475725942 2 13.90479008524053 20.211581201236257 42.52499256197944 23.358636151543774 3 18.969438903006683 24.336531379712323 29.733702076047496 26.9603276412335 4 11.084068388121926 16.911486351458276 39.544945709657554 32.459499550762246 5 12.659942283551137 38.103412156181946 36.52359894700773 12.713046613259193 6 31.291412238534495 37.82593867740678 18.89664804469274 11.986001039365988 7 27.514866123475123 31.47221842292086 23.095084572448336 17.917830881155684 8 24.13018842118289 37.46116621835495 21.26772350998769 17.140921850474474 9 25.54872780892125 16.180243850156224 20.65645969067276 37.614568650249765 10 13.234169104354704 30.625537008733446 35.67687057703771 20.46342330987414 11 33.85872292330102 23.736718431561048 24.09657728114947 18.307981363988457 12 21.918439992724817 26.051816197677134 32.422473822329614 19.607269987268428 13 28.038674706034232 22.6924844471365 26.114033221663423 23.15480762516584 14 19.612106764016186 23.06085860718299 28.86659044996005 28.46044417884077 15 22.853936817353627 31.210356575652565 23.634869649660118 22.30083695733369 16 20.788207419244305 30.08946420538604 28.417267939530056 20.7050604358396 17 20.805834513400804 29.505151156899174 28.2677042599353 21.421310069764722 18 20.213315318884966 29.417085361853324 29.853751692914283 20.51584762634743 19 22.588299109375914 28.224046200718476 28.26578405451581 20.9218706353898 20 22.0075903362061 28.57938592004001 29.080536487691283 20.332487256062606 21 22.27370014192966 27.917271572820958 28.078850532155027 21.73017775309436 22 21.24347787959837 29.01383428414603 28.743611854060404 20.9990759821952 23 21.277231501254494 28.520181556874558 28.416573966563003 21.78601297530794 24 21.9065497659116 28.17602668429164 29.016568879477354 20.90085467031941 25 21.518072602201386 28.390663111365168 28.651627005134834 21.439637281298616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 178.0 2 251.0 3 324.0 4 324.0 5 324.0 6 492.5 7 661.0 8 661.0 9 661.0 10 1067.0 11 1473.0 12 1473.0 13 1473.0 14 2456.5 15 3440.0 16 3440.0 17 3440.0 18 5955.0 19 8470.0 20 8470.0 21 8470.0 22 13040.5 23 17611.0 24 17611.0 25 17611.0 26 24632.5 27 31654.0 28 31654.0 29 31654.0 30 40464.5 31 49275.0 32 49275.0 33 49275.0 34 57016.0 35 64757.0 36 64757.0 37 64757.0 38 70443.5 39 76130.0 40 76130.0 41 76130.0 42 79590.0 43 83050.0 44 83050.0 45 83050.0 46 83109.5 47 83169.0 48 83169.0 49 83169.0 50 78183.0 51 73197.0 52 73197.0 53 73197.0 54 63031.5 55 52866.0 56 52866.0 57 52866.0 58 43703.0 59 34540.0 60 34540.0 61 34540.0 62 26869.5 63 19199.0 64 19199.0 65 19199.0 66 14330.0 67 9461.0 68 9461.0 69 9461.0 70 6725.0 71 3989.0 72 3989.0 73 3989.0 74 2776.0 75 1563.0 76 1563.0 77 1563.0 78 1083.0 79 603.0 80 603.0 81 603.0 82 393.0 83 183.0 84 183.0 85 183.0 86 126.0 87 69.0 88 69.0 89 69.0 90 45.0 91 21.0 92 21.0 93 21.0 94 16.0 95 11.0 96 11.0 97 11.0 98 9.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22032725985627585 2 0.13297570067965359 3 0.13622297053752058 4 0.06738084955074022 5 0.02159434455481554 6 0.023055615990855687 7 0.28478556653493575 8 0.28348665859178895 9 0.018509438189841892 10 0.036207058915217034 11 0.01704816675380174 12 0.01785998421826849 13 0.01607398579644164 14 0.018509438189841892 15 0.01623634928933499 16 0.02029543661166874 17 0.01915889216141529 18 0.01623634928933499 19 0.02500397790557589 20 0.019321255654308638 21 0.016885803260908393 22 0.01834707469694854 23 0.01899652866852194 24 0.01769762072537514 25 0.017535257232481793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 615902.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.63301810439769 #Duplication Level Percentage of deduplicated Percentage of total 1 95.96126876197938 87.93220677787428 2 3.250565575607834 5.957182684784092 3 0.37951801131895135 1.0432914240640343 4 0.13686994012150933 0.5016722280440837 5 0.06531529558126363 0.2992518831246009 6 0.04482801173749086 0.24646356066753916 7 0.023378858013996452 0.1499592723759676 8 0.018776891202109118 0.13764665691737366 9 0.017453243930755025 0.143936407537861 >10 0.08696868541914696 1.5099336036042026 >50 0.008502679490474879 0.5372707813500978 >100 0.006022636494891984 1.2305130447866355 >500 5.314091022297747E-4 0.3106716748692519 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 823 0.13362515465122698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGTCG 45 3.4298697E-5 16.94746 3 CGGTCGT 35 0.0021336847 16.324865 4 GGCTCGG 40 0.005338775 14.223928 17 GGTCGTC 50 0.0014763189 13.325452 5 ACTGTTC 110 2.6884663E-9 12.968764 8 CCTACCC 60 3.9681297E-4 12.710596 3 GTCCTAG 100 1.3662248E-7 12.397893 1 GTCCTAC 385 0.0 12.137797 1 ATTAGGC 55 0.0029863438 12.132842 3 ATTACCC 55 0.0029863438 12.132842 3 TTAGAAC 80 2.760166E-5 11.916183 3 GTGCATT 350 0.0 11.378218 11 CCTAGCA 85 5.126401E-5 11.215232 2 CCAGGGG 60 0.005719455 11.121772 3 CTCGGTC 60 0.005719455 11.121772 2 GTAGGAC 300 0.0 11.121771 3 CCTACAG 430 0.0 11.084822 3 GTGTAGC 155 2.910383E-10 11.07504 1 TAGCCCT 155 2.9831426E-10 11.05878 4 TCCTACA 475 0.0 11.038149 2 >>END_MODULE