FastQCFastQC Report
Thu 26 May 2016
SRR1926978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615902
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14130.22941961545830344No Hit
GGTATCAACGCAGAGTACTTTTTTT9360.15197222934817553No Hit
CACACACACACACACACACACACAC7480.12144789268422575No Hit
TATCAACGCAGAGTACTTTTTTTTT7030.11414153550402498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC508.801489E-515.17941218
TAGGACC1250.014.4780344
GTATTAA602.483215E-514.2980211
CCCTGTA759.286159E-713.98028852
GTGACAC551.9126001E-413.8557073
TCGAACT500.001513129713.28198519
CCTCGAA655.5117118E-513.13602917
CTAAGGG603.99334E-412.7010653
ACAAGAC603.99334E-412.7010653
AGGACGT2500.012.57302955
GGACGTG2300.012.4219176
TAGGACG2700.012.3472514
GTCCTAC2950.012.2785271
GTACAAG701.05515755E-412.2554471
GTAGGAC3450.012.1488453
GGCCTCG550.003096098812.074532515
GCATTTC2300.011.96203613
TCCTACA3700.011.8506142
CCCATAC657.831066E-411.724063
TAAGACT907.2251714E-611.6416944
Produced by FastQC (version 0.11.5)