##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926978_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 615902 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.724769525021838 31.0 30.0 33.0 25.0 34.0 2 30.059030495111234 31.0 30.0 34.0 26.0 34.0 3 30.118392211747974 31.0 30.0 34.0 26.0 34.0 4 33.67718727979451 35.0 35.0 37.0 28.0 37.0 5 33.640921770021855 37.0 35.0 37.0 28.0 37.0 6 33.56703826258074 37.0 35.0 37.0 28.0 37.0 7 33.54599108299697 37.0 35.0 37.0 28.0 37.0 8 33.5692918678621 37.0 35.0 37.0 28.0 37.0 9 35.0860315439794 39.0 35.0 39.0 28.0 39.0 10 34.98952593107345 39.0 35.0 39.0 28.0 39.0 11 34.99942848050502 39.0 35.0 39.0 28.0 39.0 12 34.892814116531525 39.0 35.0 39.0 27.0 39.0 13 34.906139288393284 39.0 35.0 39.0 27.0 39.0 14 35.997738276544 40.0 36.0 41.0 27.0 41.0 15 35.977684761536736 40.0 36.0 41.0 27.0 41.0 16 35.94686329968079 40.0 36.0 41.0 27.0 41.0 17 35.91267928988703 40.0 36.0 41.0 27.0 41.0 18 35.88819649879364 40.0 36.0 41.0 27.0 41.0 19 35.971930599348596 40.0 36.0 41.0 27.0 41.0 20 35.91690398797211 40.0 36.0 41.0 27.0 41.0 21 35.86757958246604 40.0 36.0 41.0 27.0 41.0 22 35.78204324713997 40.0 36.0 41.0 27.0 41.0 23 35.70512029511188 40.0 36.0 41.0 27.0 41.0 24 35.621253056492755 40.0 36.0 41.0 27.0 41.0 25 35.50570545314027 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11208043674691837 1101 2 0.16362648837299432 1101 3 0.17469548898909792 1101 4 0.3041649050634021 1101 5 0.1752291164670865 1101 6 0.3542123393483152 1101 7 -0.02804213310367487 1101 8 -0.03562408002589024 1101 9 0.17593010106276807 1101 10 0.21282192405642064 1101 11 0.25209713405439516 1101 12 0.048304866087974574 1101 13 0.10960499139400781 1101 14 0.21902724708185417 1101 15 0.31180425112876264 1101 16 0.31991055270612634 1101 17 0.2451324959991581 1101 18 0.15553643218412816 1101 19 0.1831734028220211 1101 20 0.11426951464392943 1101 21 0.2649712225413765 1101 22 -0.029128972902668693 1101 23 0.05945345206681196 1101 24 0.1851077789925455 1101 25 -0.02323282765497936 1102 1 -0.678885874930657 1102 2 -0.47161390149722493 1102 3 -0.42174559090008046 1102 4 -0.5486191693750087 1102 5 -0.4124441605051956 1102 6 -0.6539982685051626 1102 7 -0.5895557552755264 1102 8 -0.662742965730807 1102 9 -0.568968415776645 1102 10 -0.5362383290455739 1102 11 -0.6312744544623996 1102 12 -0.16373577469822465 1102 13 -0.3148730986030941 1102 14 -0.30372033048587355 1102 15 -0.437427081054544 1102 16 -0.5921405429128228 1102 17 -0.49768574673354493 1102 18 -0.433914166355045 1102 19 -0.6417925001666376 1102 20 -0.645123202139402 1102 21 -0.43202039933583336 1102 22 -0.6566803085075534 1102 23 -0.5303595014709543 1102 24 -0.6753320843342792 1102 25 -0.778448363536441 1103 1 -0.27830000504071606 1103 2 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9 0.41839083392765275 1107 10 0.443420983059184 1107 11 0.44565041351871315 1107 12 0.6376631132696602 1107 13 0.3478989720677532 1107 14 0.5474149853815362 1107 15 0.5161404558036438 1107 16 0.5716466994524367 1107 17 0.5194231560428122 1107 18 0.547947788921519 1107 19 0.654705157487669 1107 20 0.6983377882583781 1107 21 0.4868194177469931 1107 22 0.6630661779738958 1107 23 0.5526354412975749 1107 24 0.6843460410440798 1107 25 0.5441410693644357 1108 1 0.5950069709392167 1108 2 0.35265954647419306 1108 3 0.5804158813713194 1108 4 0.5478007921073242 1108 5 0.7378822332441501 1108 6 0.7506373461535105 1108 7 0.6861600465735052 1108 8 0.49808429489470996 1108 9 0.3236178486960739 1108 10 0.5302663987461571 1108 11 0.4283364347833043 1108 12 0.3671103787960348 1108 13 0.4532318048511428 1108 14 0.6538839348194045 1108 15 0.32240018414552907 1108 16 0.5512668522957966 1108 17 0.5651982290945625 1108 18 0.5621036926666392 1108 19 0.47854889808863277 1108 20 0.6865507151802817 1108 21 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0.3656764049402881 2214 12 0.3762530261627077 2214 13 0.3336204859525935 2214 14 0.3766284614507427 2214 15 0.1767582034517119 2214 16 0.14964930699246537 2214 17 0.2554454188081081 2214 18 0.3399660291049287 2214 19 0.03884283224218876 2214 20 0.037819765369974334 2214 21 0.14483697350362945 2214 22 0.2551614686558281 2214 23 0.27907467952638143 2214 24 0.19385453444439804 2214 25 0.3709999913712778 2215 1 0.6127064962321995 2215 2 0.35815761797693924 2215 3 0.25985884235633705 2215 4 0.3422582168217616 2215 5 0.35318411723762466 2215 6 0.41592254122842576 2215 7 0.4429376018323694 2215 8 0.193455901481272 2215 9 0.09086306453941972 2215 10 0.4554536854988598 2215 11 0.14506985712420573 2215 12 0.35413010880642304 2215 13 0.22622542597560624 2215 14 0.17684046945818466 2215 15 0.084332760914549 2215 16 0.2576995032377951 2215 17 0.16403371344406992 2215 18 -0.0749401435594379 2215 19 0.16879329802929277 2215 20 -0.009748053548392477 2215 21 0.10615437865172339 2215 22 0.004138864976816592 2215 23 0.14615787493628574 2215 24 0.3229853410205905 2215 25 0.04274421040943821 2216 1 0.002422595332571831 2216 2 0.0967856854254805 2216 3 -1.5978197879484064 2216 4 -0.9090400027628078 2216 5 -0.09979737209401662 2216 6 0.05295977451936551 2216 7 0.003753743323585468 2216 8 -0.027471044964961777 2216 9 0.014229839538245415 2216 10 0.10794002622704113 2216 11 -0.1501352674577774 2216 12 -0.08388505769539023 2216 13 -0.2549224238666099 2216 14 -0.34794639880049516 2216 15 -0.3778010292978706 2216 16 -0.06137107751676041 2216 17 -0.20407188685737765 2216 18 -0.015030695111413195 2216 19 -0.12341208560783912 2216 20 -0.17463665983165555 2216 21 0.030130522026972528 2216 22 8.359117480480904E-4 2216 23 -0.36575277458563704 2216 24 -0.1338849194475955 2216 25 -0.287286169695129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16169.0 3 13.0 4 263.0 5 1801.0 6 2451.0 7 1732.0 8 1443.0 9 1228.0 10 1046.0 11 1009.0 12 885.0 13 848.0 14 788.0 15 819.0 16 888.0 17 892.0 18 1008.0 19 1205.0 20 1601.0 21 2013.0 22 2573.0 23 3034.0 24 3767.0 25 4339.0 26 5130.0 27 5627.0 28 6534.0 29 8178.0 30 10141.0 31 12956.0 32 16917.0 33 22069.0 34 30129.0 35 41601.0 36 61049.0 37 104216.0 38 239540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41582275394594 19.734553552560996 10.32152668281344 28.528097010679627 2 13.860520305909315 21.200230813618003 40.58304820266086 24.35620067781182 3 18.326439603947534 24.749056882605228 29.15877013919 27.76573337425724 4 11.456692785540557 17.901711635942082 38.581787362275165 32.059808216242196 5 12.39171698900567 38.78914628740483 35.83150798148289 12.987628742106605 6 30.747800132619297 39.090270729096574 18.0047801598926 12.157148978391527 7 27.936598159982463 32.35897394255832 21.436123133389092 18.268304764070127 8 24.510663587914923 38.690945936729705 19.886040718765123 16.91234975659025 9 25.59464269476091 16.471050546252876 20.069109757206512 37.865197001779705 10 13.284048360541279 31.19972591796374 34.82903970617367 20.687186015321316 11 33.90732089883043 23.87382340667709 23.714535768274644 18.504319926217836 12 21.935343504881466 26.43346901531253 31.966663473512792 19.66452400629322 13 28.001272940118234 22.709316655219947 26.054843585859324 23.23456681880249 14 19.818575033040972 23.220252572649542 28.458585943867693 28.50258645044179 15 22.422639401703574 31.469015168490067 23.628227485135138 22.480117944671218 16 21.061860691670724 30.23006981652866 27.748173404773503 20.959896087027115 17 20.65702011034223 29.725021188435825 28.107231345246486 21.510727355975465 18 20.283649943172595 29.5237863289495 29.640038967364834 20.55252476051307 19 21.88874510676229 28.486396352660577 28.50182090952929 21.123037631047847 20 21.820242378583394 28.723031161105123 29.09809448348423 20.358631976827258 21 21.92723776984609 27.977603627069215 28.270386837584077 21.82477176550062 22 21.371581842565863 29.04634178814162 28.49219518689662 21.0898811823959 23 21.16927693042075 28.702455910193503 28.376754743449446 21.751512415936304 24 21.08095119028677 28.357595851288032 29.455173063247074 21.10627989517813 25 21.44995827014318 28.520327473833596 28.613042447545407 21.41667180847782 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 265.0 3 284.0 4 284.0 5 284.0 6 439.0 7 594.0 8 594.0 9 594.0 10 957.0 11 1320.0 12 1320.0 13 1320.0 14 2286.0 15 3252.0 16 3252.0 17 3252.0 18 5694.0 19 8136.0 20 8136.0 21 8136.0 22 12660.5 23 17185.0 24 17185.0 25 17185.0 26 24097.5 27 31010.0 28 31010.0 29 31010.0 30 39841.5 31 48673.0 32 48673.0 33 48673.0 34 56308.5 35 63944.0 36 63944.0 37 63944.0 38 69668.0 39 75392.0 40 75392.0 41 75392.0 42 78845.0 43 82298.0 44 82298.0 45 82298.0 46 83384.0 47 84470.0 48 84470.0 49 84470.0 50 79929.0 51 75388.0 52 75388.0 53 75388.0 54 64524.5 55 53661.0 56 53661.0 57 53661.0 58 44302.5 59 34944.0 60 34944.0 61 34944.0 62 27217.0 63 19490.0 64 19490.0 65 19490.0 66 14480.0 67 9470.0 68 9470.0 69 9470.0 70 6727.5 71 3985.0 72 3985.0 73 3985.0 74 2694.5 75 1404.0 76 1404.0 77 1404.0 78 976.5 79 549.0 80 549.0 81 549.0 82 351.0 83 153.0 84 153.0 85 153.0 86 99.5 87 46.0 88 46.0 89 46.0 90 26.5 91 7.0 92 7.0 93 7.0 94 4.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11560280694006514 2 0.1115437196177314 3 0.02029543661166874 4 0.0 5 0.006332176222840646 6 0.3433987874694351 7 0.023867433455322436 8 0.011040717516747794 9 0.011365444502534494 10 0.004546177801013797 11 0.005682722251267247 12 1.6236349289334992E-4 13 8.118174644667496E-4 14 0.0 15 0.0032472698578669985 16 3.2472698578669984E-4 17 0.0 18 3.2472698578669984E-4 19 1.6236349289334992E-4 20 9.741809573600995E-4 21 0.01428798737461479 22 0.0 23 0.0 24 0.0 25 0.005845085744160597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 615902.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.93679560827125 #Duplication Level Percentage of deduplicated Percentage of total 1 94.42903256655059 83.98215568858099 2 4.54382013750718 8.082256057004463 3 0.49108634204485785 1.3102693688537135 4 0.18601664127882717 0.6617489602060865 5 0.08219499887842405 0.3655079907636242 6 0.05629994481447134 0.3004282010832954 7 0.037601989847743836 0.2340940339887159 8 0.024138675828626294 0.17174531827398837 9 0.021228845099841964 0.1699222911879866 >10 0.11013569696416581 1.8395554926377826 >50 0.009313457686483208 0.5681208033081872 >100 0.007304562799892026 1.1505202476160734 >500 0.0016435266290385048 0.9341886592379682 >1k 1.826140698931672E-4 0.22948688725717126 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1413 0.22941961545830344 No Hit GGTATCAACGCAGAGTACTTTTTTT 936 0.15197222934817553 No Hit CACACACACACACACACACACACAC 748 0.12144789268422575 No Hit TATCAACGCAGAGTACTTTTTTTTT 703 0.11414153550402498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAC 50 8.801489E-5 15.179412 18 TAGGACC 125 0.0 14.478034 4 GTATTAA 60 2.483215E-5 14.298021 1 CCCTGTA 75 9.286159E-7 13.9802885 2 GTGACAC 55 1.9126001E-4 13.855707 3 TCGAACT 50 0.0015131297 13.281985 19 CCTCGAA 65 5.5117118E-5 13.136029 17 CTAAGGG 60 3.99334E-4 12.701065 3 ACAAGAC 60 3.99334E-4 12.701065 3 AGGACGT 250 0.0 12.5730295 5 GGACGTG 230 0.0 12.421917 6 TAGGACG 270 0.0 12.347251 4 GTCCTAC 295 0.0 12.278527 1 GTACAAG 70 1.05515755E-4 12.255447 1 GTAGGAC 345 0.0 12.148845 3 GGCCTCG 55 0.0030960988 12.0745325 15 GCATTTC 230 0.0 11.962036 13 TCCTACA 370 0.0 11.850614 2 CCCATAC 65 7.831066E-4 11.72406 3 TAAGACT 90 7.2251714E-6 11.641694 4 >>END_MODULE