FastQCFastQC Report
Thu 26 May 2016
SRR1926977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648341
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10110.15593645936320547No Hit
GTATCAACGCAGAGTACTTTTTTTT9390.14483119222754692No Hit
GTCCTACAGTGTGCATTTCTCATTT8970.1383531197317461No Hit
CTGTAGGACGTGGAATATGGCAAGA8760.13511408348384568No Hit
GTCCTAAAGTGTGTATTTCTCATTT6860.10580851743141341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC801.9139425E-613.1103923
CTAAGGC751.4209205E-512.7131073
GTCCTAC6200.012.6154761
GTATAGG853.7591726E-612.343981
TAGGACC2550.012.3258064
GTAGGAC5100.011.778323
TTATACC657.8410906E-411.7224454
GATATAC2200.011.7063791
GTTCAGG907.1136237E-611.6582041
CCTGCAC907.1444283E-611.6536823
TCCTACA7300.011.6246572
GGCGAGG1800.011.58895419
CCTACAG7100.011.4149383
AGGACGT3600.011.3685425
GACGTGG2450.011.2660257
ATTTCTC6350.011.1990615
AACCATT855.2108735E-511.1974697
GTCCTAA2300.011.1974051
TGTAGGA5650.011.1380322
CTACAGC600.00575795411.11194
Produced by FastQC (version 0.11.5)