##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926977_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 648341 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.884764344688982 30.0 27.0 31.0 10.0 31.0 2 27.548038763551897 31.0 28.0 31.0 16.0 34.0 3 27.36178800970477 31.0 27.0 31.0 10.0 34.0 4 30.996932169953773 35.0 32.0 37.0 16.0 37.0 5 30.805738029833066 35.0 32.0 37.0 10.0 37.0 6 30.474060718048065 35.0 30.0 37.0 10.0 37.0 7 30.530753106775602 35.0 31.0 37.0 10.0 37.0 8 30.19290311734103 35.0 30.0 37.0 10.0 37.0 9 32.29626539120617 37.0 32.0 39.0 10.0 39.0 10 32.43664367979196 37.0 32.0 39.0 10.0 39.0 11 32.60659436932108 37.0 33.0 39.0 10.0 39.0 12 32.45783160404787 37.0 32.0 39.0 10.0 39.0 13 32.58948300354289 37.0 33.0 39.0 10.0 39.0 14 33.53415563723411 39.0 33.0 40.0 10.0 41.0 15 33.0950333235134 38.0 32.0 40.0 8.0 41.0 16 33.15863257144003 38.0 32.0 40.0 2.0 41.0 17 33.39499738563503 38.0 33.0 40.0 2.0 41.0 18 33.44227343327046 39.0 33.0 40.0 2.0 41.0 19 32.82226791148485 39.0 32.0 40.0 2.0 41.0 20 33.09203952858141 39.0 32.0 40.0 2.0 41.0 21 33.0891938038779 39.0 33.0 40.0 2.0 41.0 22 33.09531558238643 39.0 33.0 40.0 2.0 41.0 23 33.07465824311589 39.0 33.0 40.0 2.0 41.0 24 31.765478351669877 38.0 31.0 40.0 2.0 41.0 25 32.33906385682843 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5208894178527679 1101 2 -0.690450610241605 1101 3 -1.43773150585017 1101 4 -0.981454464720855 1101 5 -0.9975898780069699 1101 6 -2.917634732276653 1101 7 -1.0392549848037227 1101 8 -0.6398876035011227 1101 9 -0.7832422332595321 1101 10 -9.602135211350046 1101 11 -4.47296562654029 1101 12 -2.2983585719068174 1101 13 -1.5465475554755521 1101 14 -1.0224784499515565 1101 15 -2.432431536346499 1101 16 -0.7886008187586171 1101 17 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0.23662665344711442 1103 4 0.23755228480498047 1103 5 0.04226411184225398 1103 6 0.791141770472958 1103 7 0.7960046653228972 1103 8 0.7826316543689096 1103 9 0.6377967910790261 1103 10 0.753807444169837 1103 11 0.3317134795978589 1103 12 0.13995055001907275 1103 13 0.3979071315774405 1103 14 0.23912024464564752 1103 15 0.6839792417032413 1103 16 0.6131008670334737 1103 17 0.20653383142308002 1103 18 0.04280869699033474 1103 19 -1.2076179340248814 1103 20 -0.060163741925450154 1103 21 0.2700210948433295 1103 22 0.30409227590532595 1103 23 0.1417395317259107 1103 24 1.6596455182911605 1103 25 1.05176313342362 1104 1 -0.8183544692107958 1104 2 -0.2599090603383196 1104 3 -1.5026392650112967 1104 4 -0.7166881611592828 1104 5 -0.8502703008734791 1104 6 -2.119339700904586 1104 7 -0.36390951700898455 1104 8 -0.08460579072618302 1104 9 -0.10584565927128864 1104 10 -0.16110237114664727 1104 11 -0.27147391294501233 1104 12 -0.1331105691634349 1104 13 -0.22188337857031826 1104 14 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1.0756414152144274 2215 2 0.17740072751835712 2215 3 0.8523200216224076 2215 4 1.1682878979162474 2215 5 1.4476170183803916 2215 6 1.6426495506525498 2215 7 0.8877027969886306 2215 8 0.07989923196846149 2215 9 0.4215339487501808 2215 10 0.3437747657223724 2215 11 0.17688875234154722 2215 12 0.1528686018041654 2215 13 0.40374297176346374 2215 14 0.562824065408968 2215 15 0.7879115670278125 2215 16 0.8288670421124422 2215 17 0.24836958130596543 2215 18 -0.5052146990472437 2215 19 0.7383394015058329 2215 20 -3.2634727874777347 2215 21 -1.6086425316177468 2215 22 -0.6201763157231994 2215 23 -0.5136383022404871 2215 24 -0.21667681122874782 2215 25 0.15530414515738755 2216 1 0.0825256051376364 2216 2 -0.7440615134291519 2216 3 -0.12644919533874344 2216 4 -0.5166652668089498 2216 5 -0.23105088180792066 2216 6 -1.6258519794551027 2216 7 -9.65512468315794 2216 8 -10.528860957961651 2216 9 -5.485263250281182 2216 10 -3.075702564002448 2216 11 -1.9888703208602507 2216 12 -1.7836884574894505 2216 13 -1.5920559204834746 2216 14 -1.5251342549073748 2216 15 -2.786391427081316 2216 16 -1.8450094549852736 2216 17 -1.947761222617597 2216 18 -1.8178960379475306 2216 19 -1.0404966120329924 2216 20 -2.0237567851234566 2216 21 -4.8306764269973925 2216 22 -3.2239025981916 2216 23 -2.818360147137799 2216 24 -3.1575523469390276 2216 25 -2.3441495727539348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47122.0 3 8.0 4 418.0 5 2083.0 6 2583.0 7 2139.0 8 1713.0 9 1588.0 10 1493.0 11 1400.0 12 1417.0 13 1392.0 14 1406.0 15 1417.0 16 1415.0 17 1653.0 18 1935.0 19 2300.0 20 3035.0 21 3836.0 22 4810.0 23 5490.0 24 6449.0 25 7309.0 26 8502.0 27 9139.0 28 10106.0 29 12133.0 30 14420.0 31 17083.0 32 22310.0 33 28585.0 34 38087.0 35 52425.0 36 75245.0 37 118527.0 38 137368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30656443472279 18.71060991350992 12.587668787051994 25.395156864715297 2 13.710738644788217 19.999351307270302 43.29020041516335 22.999709632778135 3 19.119932041084255 24.500579195304656 29.636728704919296 26.74276005869179 4 10.938430906481038 16.724752658630322 39.8902591489296 32.44655728595904 5 12.36798015421214 38.533287668274205 36.65330655139325 12.445425626120414 6 31.20230052807857 37.89037008696404 18.97812245930648 11.929206925650918 7 27.526716069547625 31.382429959738356 23.03482794835744 18.056026022356583 8 23.919371203028916 38.20232365350589 21.180138825305303 16.698166318159885 9 25.780365368654568 15.904890608464385 20.604052315410463 37.71069170747059 10 13.245561386103477 30.52896150244014 35.91485514234269 20.310621969113697 11 34.029115804510376 23.30498473556821 24.46335379389392 18.2025456660275 12 22.162315594286603 25.888147897894427 32.80759482345028 19.14194168436869 13 28.340606840336108 22.819220570064005 26.011696231197252 22.828476358402636 14 19.36643346934368 23.343077741868303 28.921764483559205 28.368724305228817 15 22.541427429879583 32.120874611641945 23.557560293840883 21.78013766463759 16 20.421738882263917 29.912637819509087 29.20161488134448 20.464008416882514 17 20.4684190471489 29.760437380345962 28.7058620258769 21.065281546628245 18 19.942922374429223 29.244261384672342 30.609033691225473 20.20378254967296 19 22.652751101134687 28.05698902336488 28.32897507694444 20.96128479855599 20 22.173388539905467 28.654710042083696 29.329159930395292 19.842741487615545 21 21.928881388249124 27.95827477743617 28.15002445078296 21.96281938353174 22 21.136722654851965 29.562255488775623 28.729066136815213 20.571955719557195 23 20.949946469286946 29.01472634544125 28.72100162597613 21.31432555929568 24 21.910230224794674 28.159497466971906 29.5213471636873 20.40892514454612 25 21.409828225874875 28.564023849375612 29.0579805163251 20.968167408424414 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 262.5 3 333.0 4 333.0 5 333.0 6 499.5 7 666.0 8 666.0 9 666.0 10 1073.0 11 1480.0 12 1480.0 13 1480.0 14 2501.0 15 3522.0 16 3522.0 17 3522.0 18 6020.5 19 8519.0 20 8519.0 21 8519.0 22 13443.0 23 18367.0 24 18367.0 25 18367.0 26 26063.5 27 33760.0 28 33760.0 29 33760.0 30 43955.5 31 54151.0 32 54151.0 33 54151.0 34 62541.0 35 70931.0 36 70931.0 37 70931.0 38 76652.5 39 82374.0 40 82374.0 41 82374.0 42 85035.5 43 87697.0 44 87697.0 45 87697.0 46 88100.0 47 88503.0 48 88503.0 49 88503.0 50 82715.5 51 76928.0 52 76928.0 53 76928.0 54 65201.5 55 53475.0 56 53475.0 57 53475.0 58 43711.5 59 33948.0 60 33948.0 61 33948.0 62 26393.5 63 18839.0 64 18839.0 65 18839.0 66 13821.5 67 8804.0 68 8804.0 69 8804.0 70 6217.5 71 3631.0 72 3631.0 73 3631.0 74 2526.0 75 1421.0 76 1421.0 77 1421.0 78 962.0 79 503.0 80 503.0 81 503.0 82 348.0 83 193.0 84 193.0 85 193.0 86 130.0 87 67.0 88 67.0 89 67.0 90 46.5 91 26.0 92 26.0 93 26.0 94 16.5 95 7.0 96 7.0 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22303078164114254 2 0.136502241875803 3 0.13742768080377457 4 0.06956215941919454 5 0.02205629444998851 6 0.02190205462865992 7 0.2807164748180356 8 0.27346720321559176 9 0.018354538738102326 10 0.034241240334947194 11 0.01573246177551628 12 0.016966380346145005 13 0.015115502490201915 14 0.016349421060830642 15 0.015269742311530506 16 0.01850877855943092 17 0.018200298916773733 18 0.015578221954187687 19 0.022519013913974283 20 0.016812140524816416 21 0.015423982132859096 22 0.016812140524816416 23 0.017737579452787963 24 0.016195181239502053 25 0.016349421060830642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 648341.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.10743289169287 #Duplication Level Percentage of deduplicated Percentage of total 1 95.28708664011491 84.90787678228962 2 3.685292650264391 6.567739350393663 3 0.4789236287371461 1.2802696532382383 4 0.16312183999780486 0.5814147364309543 5 0.09421418716173856 0.4197592179980005 6 0.06176440481798767 0.3302200534448512 7 0.037544925957711806 0.23418723791402674 8 0.027961153378384333 0.19932372785909702 9 0.022485562141543243 0.18032676476035991 >10 0.12205250233332741 2.1438469651051992 >50 0.008997715608270078 0.5712535667250379 >100 0.008478437457782498 1.3070008081207733 >500 0.0019033226933151488 1.1209031344868006 >1k 1.7302933575592263E-4 0.15587800123330883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1011 0.15593645936320547 No Hit GTATCAACGCAGAGTACTTTTTTTT 939 0.14483119222754692 No Hit GTCCTACAGTGTGCATTTCTCATTT 897 0.1383531197317461 No Hit CTGTAGGACGTGGAATATGGCAAGA 876 0.13511408348384568 No Hit GTCCTAAAGTGTGTATTTCTCATTT 686 0.10580851743141341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5423982132859097E-4 0.0 4 0.0 0.0 0.0 1.5423982132859097E-4 0.0 5 0.0 0.0 0.0 1.5423982132859097E-4 0.0 6 0.0 0.0 0.0 1.5423982132859097E-4 0.0 7 0.0 0.0 0.0 1.5423982132859097E-4 0.0 8 0.0 0.0 0.0 1.5423982132859097E-4 0.0 9 0.0 0.0 0.0 1.5423982132859097E-4 0.0 10 0.0 0.0 0.0 1.5423982132859097E-4 0.0 11 1.5423982132859097E-4 0.0 0.0 1.5423982132859097E-4 0.0 12 1.5423982132859097E-4 0.0 0.0 1.5423982132859097E-4 0.0 13 1.5423982132859097E-4 0.0 0.0 1.5423982132859097E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGAC 80 1.9139425E-6 13.110392 3 CTAAGGC 75 1.4209205E-5 12.713107 3 GTCCTAC 620 0.0 12.615476 1 GTATAGG 85 3.7591726E-6 12.34398 1 TAGGACC 255 0.0 12.325806 4 GTAGGAC 510 0.0 11.77832 3 TTATACC 65 7.8410906E-4 11.722445 4 GATATAC 220 0.0 11.706379 1 GTTCAGG 90 7.1136237E-6 11.658204 1 CCTGCAC 90 7.1444283E-6 11.653682 3 TCCTACA 730 0.0 11.624657 2 GGCGAGG 180 0.0 11.588954 19 CCTACAG 710 0.0 11.414938 3 AGGACGT 360 0.0 11.368542 5 GACGTGG 245 0.0 11.266025 7 ATTTCTC 635 0.0 11.19906 15 AACCATT 85 5.2108735E-5 11.197469 7 GTCCTAA 230 0.0 11.197405 1 TGTAGGA 565 0.0 11.138032 2 CTACAGC 60 0.005757954 11.1119 4 >>END_MODULE