FastQCFastQC Report
Thu 26 May 2016
SRR1926977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648341
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15050.23213093109952943No Hit
CTGTAGGACGTGGAATATGGCAAGA11150.17197740078137894No Hit
GTCCTACAGTGGACATTTCTAAATT10750.1658078079282353No Hit
GGTATCAACGCAGAGTACTTTTTTT10430.1608721336457204No Hit
GTCCTACAGTGTGCATTTCTCATTT9580.14776174883279014No Hit
TATCAACGCAGAGTACTTTTTTTTT8150.12570545438280165No Hit
GTCCTAAAGTGTGTATTTCTCATTT7750.119535861529658No Hit
CACACACACACACACACACACACAC7700.11876466242301505No Hit
CTTTAGGACGTGAAATATGGCGAGG7490.11552562617511465No Hit
GTACATGGGAAGCAGTGGTATCAAC7280.11228658992721423No Hit
CTGTAGGACCTGGAATATGGCGAGA7170.11058995189259972No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6920.10673395635938496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC350.002192087516.25920713
CTCGAAC704.4949593E-714.907721518
GTCTTTA456.571405E-414.8315071
TCTGGAC851.6456397E-814.5720693
GCTCTAA400.005149182414.3018111
CCAACGA400.005323843614.23009819
TCCAACG551.9807207E-413.798882518
TATGTCG500.001513761613.28142416
TGTCGAG500.001513761613.28142418
CCTAAAC801.929664E-613.1008513
TCGAACT802.0258176E-613.04425619
TGTAGGA7400.012.8835152
TCTTATA751.4225028E-512.7117352
TTAGGAC2450.012.4445933
TAGACTG1155.0840754E-912.4257595
CCTCGAA853.995243E-612.27694617
GGACGTG4050.012.2314476
GCCTCGA701.1033095E-412.19722716
AGGACGT4150.012.16626555
GTCCTAT550.002982903312.134871
Produced by FastQC (version 0.11.5)