FastQCFastQC Report
Thu 26 May 2016
SRR1926976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503132
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8440.16774921889285516No Hit
GTCCTACAGTGGACATTTCTAAATT7210.14330235405420447No Hit
GTCCTACAGTGTGCATTTCTCATTT5950.11825922421948912No Hit
CTGTAGGACGTGGAATATGGCAAGA5880.11686793922867161No Hit
GGTATCAACGCAGAGTACTTTTTTT5510.10951400427720757No Hit
CACACACACACACACACACACACAC5320.10573765930213144No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5310.10553890430344323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT401.302055E-518.96649219
TACTGAC350.002144095316.312147
GGATAGA400.005130350614.3088111
GGCCTCG400.00533463814.22486815
TAGGACC1550.014.135164
CCCCTGT551.8888083E-413.8752121
GTCCTAA1500.013.3548921
CTCGAAC500.001517133613.27654518
GGGTTTC905.1182906E-712.7189441
CTAGACC603.9773714E-412.7062353
GTTCTAA701.04700506E-412.2646961
AATCCCG701.10614914E-412.19274419
AGGATTG550.00302648712.1105285
TATACTG802.8102755E-511.8942685
TCCTAAA2000.011.4367552
CTAGGAC752.0093982E-411.4356123
CTATTAG600.005689082711.1290761
GGCTATA600.005689082711.1290761
CCTTTAA951.2945058E-511.0453991
GTGTTAA700.001438062910.9019521
Produced by FastQC (version 0.11.5)