FastQCFastQC Report
Thu 26 May 2016
SRR1926976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503132
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14600.29018229808479684No Hit
GGTATCAACGCAGAGTACTTTTTTT10380.20630768863836926No Hit
TATCAACGCAGAGTACTTTTTTTTT7910.15721520396237965No Hit
CACACACACACACACACACACACAC7220.14350110905289268No Hit
GTCCTACAGTGGACATTTCTAAATT7180.1427060890581398No Hit
CTGTAGGACGTGGAATATGGCAAGA7010.1393272540804401No Hit
GTCCTACAGTGTGCATTTCTCATTT6680.13276833912372896No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6040.12004801920768307No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5580.11090528926802508No Hit
CTGTAGGACCTGGAATATGGCGAGA5210.10355135431656107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1800.014.8129214
ATGCCGG400.005311492414.23419819
TGTAGGA4450.013.9135662
TCATACT500.001470519213.3316284
GTAGGAC4300.013.2873363
AGGACCT2800.013.2636095
TGGCGAG3050.012.75632918
CTGTAGG4650.012.7018561
TCAGGAC604.0024324E-412.6967883
GGACCTG2650.012.5770076
GGCGAGG1155.3751137E-912.377562519
TTGTACT853.825342E-612.3233544
GTGCTAC550.003000462212.1244981
GCGAGGA550.003091353512.0762999
GGCGAGA2250.011.80911219
GAATAAT907.2062376E-611.6433681
ATGGCGA3650.011.43935417
TTCAGAG1251.7484126E-811.430532
TATGGCG3700.011.28476816
CTAATAC855.179132E-511.2030493
Produced by FastQC (version 0.11.5)