##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926976_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 503132 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.717748026362862 31.0 30.0 33.0 25.0 34.0 2 30.037886677849947 31.0 30.0 34.0 26.0 34.0 3 30.09864806849892 31.0 30.0 34.0 26.0 34.0 4 33.66121017943601 35.0 35.0 37.0 28.0 37.0 5 33.65328581763831 37.0 35.0 37.0 28.0 37.0 6 33.5773335824396 37.0 35.0 37.0 28.0 37.0 7 33.56599063466446 37.0 35.0 37.0 28.0 37.0 8 33.56416208867653 37.0 35.0 37.0 28.0 37.0 9 35.09323398233465 39.0 35.0 39.0 28.0 39.0 10 34.991352170007076 39.0 35.0 39.0 28.0 39.0 11 34.994144677738646 39.0 35.0 39.0 28.0 39.0 12 34.904553874529945 39.0 35.0 39.0 27.0 39.0 13 34.90134994395109 39.0 35.0 39.0 27.0 39.0 14 35.9872578170341 40.0 36.0 41.0 27.0 41.0 15 35.964981356781124 40.0 36.0 41.0 27.0 41.0 16 35.93288242449297 40.0 36.0 41.0 27.0 41.0 17 35.92345945000517 40.0 36.0 41.0 27.0 41.0 18 35.90076957935492 40.0 36.0 41.0 27.0 41.0 19 35.98433611855338 40.0 36.0 41.0 27.0 41.0 20 35.950273486878196 40.0 36.0 41.0 27.0 41.0 21 35.892809441657455 40.0 36.0 41.0 27.0 41.0 22 35.80260051040284 40.0 36.0 41.0 27.0 41.0 23 35.734854869099955 40.0 36.0 41.0 27.0 41.0 24 35.65378668023501 40.0 36.0 41.0 27.0 41.0 25 35.53676967475732 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06741690870181216 1101 2 -0.1296115645442022 1101 3 -0.00832687599029569 1101 4 -0.18032494375282226 1101 5 -0.2541272265429697 1101 6 -0.25882688924073705 1101 7 -0.29282975884811435 1101 8 -0.2715356902212136 1101 9 0.10220863865905017 1101 10 -0.07718583287574887 1101 11 -0.027031193201871417 1101 12 -0.17058580512866683 1101 13 -0.07046343018554069 1101 14 -0.13878004590416282 1101 15 0.1308102710930541 1101 16 0.19523714120812485 1101 17 0.09245580915506935 1101 18 -0.028983272219441858 1101 19 -0.24704875351036293 1101 20 -0.1465981899445623 1101 21 -0.2631196958635371 1101 22 -0.4459332825620521 1101 23 -0.3443077085223649 1101 24 -0.28661367413359784 1101 25 -0.35080833162928116 1102 1 -0.6043744376593416 1102 2 -0.600957767890403 1102 3 -0.3126820883455075 1102 4 -0.3412412886691669 1102 5 -0.5711207915365364 1102 6 -0.3801293345431844 1102 7 -0.4869713289896822 1102 8 -0.6615331162186422 1102 9 -0.414162017711611 1102 10 -0.5170416887316023 1102 11 -0.5244520426227197 1102 12 -0.5430594216022868 1102 13 -0.39933859106069747 1102 14 -0.49473886186297733 1102 15 -0.4250301407473529 1102 16 -0.6311618291908445 1102 17 -0.5829624662632114 1102 18 -0.7040360392722107 1102 19 -0.7229921254537288 1102 20 -0.6171824885288615 1102 21 -0.5325199532637939 1102 22 -0.681382446011213 1102 23 -0.5340734742881281 1102 24 -0.5705518980718196 1102 25 -0.7803578811788299 1103 1 -0.4508084075832457 1103 2 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0.3296817010831319 2214 9 0.21805404928706906 2214 10 0.06103638934647648 2214 11 0.3479156667015104 2214 12 0.20867989535442177 2214 13 0.25549792247146996 2214 14 -0.08336941788483898 2214 15 0.14502283147952966 2214 16 0.0988989769569173 2214 17 0.2637118478024121 2214 18 -0.021524335021375407 2214 19 -0.03824682114321121 2214 20 -0.1108687213455326 2214 21 -0.28115834320652766 2214 22 -0.011894635219057648 2214 23 -0.16565070369144763 2214 24 -0.1980629494959132 2214 25 0.13875688576202805 2215 1 0.266547743990337 2215 2 0.20822485140319102 2215 3 0.37069655187653794 2215 4 0.3333092527124464 2215 5 0.41721843724658925 2215 6 0.2554731518607838 2215 7 0.11933957941739948 2215 8 0.2165810380977362 2215 9 0.09259910310623098 2215 10 0.21139945277982264 2215 11 -0.044122849593158264 2215 12 -0.08194955358735712 2215 13 0.055412443221776186 2215 14 -0.015812516105562224 2215 15 0.17669929698701736 2215 16 0.10701354893521398 2215 17 0.24527126332687033 2215 18 0.29236321360053097 2215 19 0.15491096699929585 2215 20 0.08501874388198871 2215 21 0.27153053019483053 2215 22 0.2350465776927777 2215 23 0.22182792649613248 2215 24 0.3015573081927485 2215 25 0.4691505668499616 2216 1 -0.45288049053557344 2216 2 -0.15682321835739543 2216 3 -1.868964408123972 2216 4 -1.4740455949953244 2216 5 -0.7049275693021784 2216 6 -0.24930846593311173 2216 7 -0.5140876851548839 2216 8 -0.3850643963051894 2216 9 -0.44716582577968467 2216 10 -0.5485037420445593 2216 11 -0.34769614607247945 2216 12 -0.46129017530861915 2216 13 -0.428266343638839 2216 14 -0.6573575951758031 2216 15 -0.8448092661820112 2216 16 -0.6868982486804782 2216 17 -0.8456761531414188 2216 18 -0.6747724753042732 2216 19 -0.5665586078376919 2216 20 -0.6343651136806372 2216 21 -0.5060537147152928 2216 22 -0.7972820400599048 2216 23 -0.8904156776740351 2216 24 -0.9434431514257966 2216 25 -1.085992564705542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13090.0 3 10.0 4 210.0 5 1509.0 6 2056.0 7 1487.0 8 1153.0 9 975.0 10 870.0 11 842.0 12 761.0 13 662.0 14 667.0 15 718.0 16 679.0 17 722.0 18 785.0 19 984.0 20 1279.0 21 1677.0 22 2120.0 23 2528.0 24 2888.0 25 3588.0 26 4155.0 27 4580.0 28 5240.0 29 6522.0 30 8342.0 31 10576.0 32 13747.0 33 17987.0 34 24258.0 35 34082.0 36 50087.0 37 86137.0 38 195159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.786972899507894 19.838141271189983 10.614740805154646 27.760145024147477 2 13.631859801592514 21.134416496551943 41.14707064436672 24.086653057488828 3 18.7152381596053 24.944440689690815 28.83061069521693 27.509710455486953 4 11.261060715676999 17.989911196266586 38.48791172097978 32.26111636707663 5 12.066635128742313 39.24404390397099 35.84907910586009 12.840241861426602 6 30.6933200903871 39.04295780406423 18.069441702117476 12.1942804034312 7 28.05823042486025 32.13547340548191 21.24420518610994 18.562090983547897 8 24.1994346846041 39.39099391351708 19.66001566345049 16.749555738428327 9 25.64452201015333 16.329812397880282 20.183626656277458 37.842038935688926 10 13.312198127646042 31.58772435451492 34.65325674305818 20.446820774780864 11 33.78863469221442 23.81984059152074 23.804535787402354 18.586988928862475 12 22.09274361048558 26.535540587006512 32.1643157122726 19.207400090235307 13 28.194273016554465 22.939138627026576 25.89226974501468 22.974318611404275 14 19.5122532943772 23.807763401109057 28.44135710452567 28.238626199988076 15 22.114052540253894 32.30090694249273 23.55347343272665 22.031567084526724 16 20.545187128575122 30.515572516049527 28.430425536143737 20.50881481923161 17 20.26227709626897 30.154512135980223 28.34127028294762 21.241940484803195 18 19.651023892480854 29.712459428893983 30.395783188804458 20.240733489820702 19 21.684455309760896 28.531393476835014 28.830520938922344 20.953630274481743 20 21.748454673742373 28.812632917933733 29.578836483612587 19.860075924711307 21 21.59906811802778 28.05386150580041 28.53868661890639 21.80838375726542 22 21.12288624058895 29.59779938465452 28.770779835112855 20.508534539643673 23 20.84224418244119 28.956019493890274 28.760444575181065 21.441291748487473 24 20.860330887321815 28.5034543618772 30.021346286859114 20.614868463941868 25 21.09796142286959 28.67901951118673 29.305341326506273 20.917677739437405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 242.0 2 252.5 3 263.0 4 263.0 5 263.0 6 413.0 7 563.0 8 563.0 9 563.0 10 905.0 11 1247.0 12 1247.0 13 1247.0 14 2032.0 15 2817.0 16 2817.0 17 2817.0 18 4804.5 19 6792.0 20 6792.0 21 6792.0 22 10547.0 23 14302.0 24 14302.0 25 14302.0 26 20008.0 27 25714.0 28 25714.0 29 25714.0 30 33604.5 31 41495.0 32 41495.0 33 41495.0 34 47415.5 35 53336.0 36 53336.0 37 53336.0 38 58185.0 39 63034.0 40 63034.0 41 63034.0 42 65470.5 43 67907.0 44 67907.0 45 67907.0 46 68807.0 47 69707.0 48 69707.0 49 69707.0 50 65886.5 51 62066.0 52 62066.0 53 62066.0 54 52076.0 55 42086.0 56 42086.0 57 42086.0 58 34434.0 59 26782.0 60 26782.0 61 26782.0 62 20520.5 63 14259.0 64 14259.0 65 14259.0 66 10472.0 67 6685.0 68 6685.0 69 6685.0 70 4627.0 71 2569.0 72 2569.0 73 2569.0 74 1726.0 75 883.0 76 883.0 77 883.0 78 577.5 79 272.0 80 272.0 81 272.0 82 171.5 83 71.0 84 71.0 85 71.0 86 52.5 87 34.0 88 34.0 89 34.0 90 19.5 91 5.0 92 5.0 93 5.0 94 3.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11825922421948912 2 0.105340149304755 3 0.013117829913422321 4 0.0 5 0.0067576699553993785 6 0.3444424127266801 7 0.019080479874068832 8 0.009937749934410852 9 0.009142729939657982 10 0.004372609971140774 11 0.004372609971140774 12 5.96264996064651E-4 13 9.93774993441085E-4 14 3.9750999737643404E-4 15 0.002583814982946821 16 3.9750999737643404E-4 17 0.0 18 5.96264996064651E-4 19 3.9750999737643404E-4 20 3.9750999737643404E-4 21 0.01272031991604589 22 0.0 23 0.0 24 0.0 25 0.0071551799527758125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 503132.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.45108319356376 #Duplication Level Percentage of deduplicated Percentage of total 1 95.2760798797677 84.2727246780196 2 3.6998415006826213 6.545099767597566 3 0.45675127277875055 1.2120043448195819 4 0.17637634517526582 0.6240271512189661 5 0.08738225773836597 0.386452767442882 6 0.05974159740335634 0.31705254012243994 7 0.03975617173909658 0.2461533517766708 8 0.025801812798897764 0.18257586323360517 9 0.023116616773260507 0.18402208140288567 >10 0.13130100611023496 2.217312127239037 >50 0.012600816533467158 0.8036742811368389 >100 0.009000577999159058 1.4602663695220193 >500 0.0018001155998302848 1.0514634360339397 >1k 4.500288999575712E-4 0.4971712404339923 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1460 0.29018229808479684 No Hit GGTATCAACGCAGAGTACTTTTTTT 1038 0.20630768863836926 No Hit TATCAACGCAGAGTACTTTTTTTTT 791 0.15721520396237965 No Hit CACACACACACACACACACACACAC 722 0.14350110905289268 No Hit GTCCTACAGTGGACATTTCTAAATT 718 0.1427060890581398 No Hit CTGTAGGACGTGGAATATGGCAAGA 701 0.1393272540804401 No Hit GTCCTACAGTGTGCATTTCTCATTT 668 0.13276833912372896 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 604 0.12004801920768307 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 558 0.11090528926802508 No Hit CTGTAGGACCTGGAATATGGCGAGA 521 0.10355135431656107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9875499868821702E-4 2 0.0 0.0 0.0 0.0 1.9875499868821702E-4 3 0.0 0.0 0.0 0.0 1.9875499868821702E-4 4 0.0 0.0 0.0 0.0 1.9875499868821702E-4 5 0.0 0.0 0.0 0.0 1.9875499868821702E-4 6 0.0 0.0 0.0 0.0 1.9875499868821702E-4 7 0.0 0.0 0.0 0.0 1.9875499868821702E-4 8 0.0 0.0 0.0 0.0 1.9875499868821702E-4 9 0.0 0.0 0.0 0.0 1.9875499868821702E-4 10 0.0 0.0 0.0 0.0 1.9875499868821702E-4 11 0.0 0.0 0.0 1.9875499868821702E-4 3.9750999737643404E-4 12 0.0 0.0 0.0 1.9875499868821702E-4 3.9750999737643404E-4 13 0.0 0.0 0.0 1.9875499868821702E-4 3.9750999737643404E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 180 0.0 14.812921 4 ATGCCGG 40 0.0053114924 14.234198 19 TGTAGGA 445 0.0 13.913566 2 TCATACT 50 0.0014705192 13.331628 4 GTAGGAC 430 0.0 13.287336 3 AGGACCT 280 0.0 13.263609 5 TGGCGAG 305 0.0 12.756329 18 CTGTAGG 465 0.0 12.701856 1 TCAGGAC 60 4.0024324E-4 12.696788 3 GGACCTG 265 0.0 12.577007 6 GGCGAGG 115 5.3751137E-9 12.3775625 19 TTGTACT 85 3.825342E-6 12.323354 4 GTGCTAC 55 0.0030004622 12.124498 1 GCGAGGA 55 0.0030913535 12.076299 9 GGCGAGA 225 0.0 11.809112 19 GAATAAT 90 7.2062376E-6 11.643368 1 ATGGCGA 365 0.0 11.439354 17 TTCAGAG 125 1.7484126E-8 11.43053 2 TATGGCG 370 0.0 11.284768 16 CTAATAC 85 5.179132E-5 11.203049 3 >>END_MODULE