FastQCFastQC Report
Thu 26 May 2016
SRR1926975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457425
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9090.1987211018199705No Hit
GGTATCAACGCAGAGTACTTTTTTT6210.13575996064928678No Hit
GTCCTACAGTGGACATTTCTAAATT5920.12942012351751653No Hit
GTCCTACAGTGTGCATTTCTCATTT5210.11389845329835492No Hit
CACACACACACACACACACACACAC5040.11018199704869651No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4930.107777231240094No Hit
CTGTAGGACGTGGAATATGGCAAGA4870.1064655407990381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGG350.002115815416.3441263
GTGTAGG851.6039849E-814.59756851
GGACTAG400.005211946614.2744376
TGTACTG954.7584763E-914.022475
GACGTGG1204.9112714E-1113.4814137
AGGACGT1700.013.4332915
GCACTGT500.001477511713.3228086
ATAAGAC655.2563882E-513.2010253
GTATAAG905.0801464E-712.7260861
CTAGACT604.0161482E-412.6911744
TAGGACG1950.012.6911744
GGACGTG1800.012.6883896
GGCGAGG854.0055565E-612.27211919
TTAGAAC550.002981673212.13427453
TCTAACA550.002981673212.13427452
TTACTCA550.003018707512.1143024
GGTGTGC802.8284632E-511.8862368
CGTGGAA1451.0004442E-1011.7729
CTAGACA657.8755914E-411.714934
TATACTG1156.8694135E-811.5837795
Produced by FastQC (version 0.11.5)