##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926975_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 457425 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.7643919768268 30.0 27.0 31.0 10.0 31.0 2 27.389589550199485 31.0 28.0 31.0 12.0 34.0 3 27.192018363666175 31.0 27.0 31.0 10.0 34.0 4 30.808707438377876 35.0 32.0 37.0 10.0 37.0 5 30.62922227687599 35.0 31.0 37.0 10.0 37.0 6 30.290060665682898 35.0 30.0 37.0 10.0 37.0 7 30.338258730939497 35.0 30.0 37.0 10.0 37.0 8 29.977355850685903 35.0 30.0 37.0 8.0 37.0 9 32.04099907088594 37.0 32.0 39.0 10.0 39.0 10 32.19303273760726 37.0 32.0 39.0 10.0 39.0 11 32.35552494944526 37.0 32.0 39.0 8.0 39.0 12 32.1909733836148 37.0 32.0 39.0 7.0 39.0 13 32.307949937148166 37.0 32.0 39.0 2.0 39.0 14 33.24032573645953 38.0 33.0 40.0 2.0 41.0 15 32.801901951139534 38.0 32.0 40.0 2.0 41.0 16 32.87083346996776 38.0 32.0 40.0 2.0 41.0 17 33.113839427228505 38.0 32.0 40.0 2.0 41.0 18 33.16539542001421 38.0 33.0 40.0 2.0 41.0 19 32.56928676832268 39.0 32.0 40.0 2.0 41.0 20 32.82055418921134 39.0 32.0 40.0 2.0 41.0 21 32.82500737825873 39.0 32.0 40.0 2.0 41.0 22 32.81906323441001 39.0 32.0 40.0 2.0 41.0 23 32.81526370443242 39.0 33.0 40.0 2.0 41.0 24 31.5169350166694 38.0 30.0 40.0 2.0 41.0 25 32.07646062195988 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8240391611231352 1101 2 -0.7934278266274575 1101 3 -1.6530601639702276 1101 4 -1.1237339638713628 1101 5 -1.1453019702449865 1101 6 -3.3080453989293375 1101 7 -1.3003953982260619 1101 8 -1.008335468549948 1101 9 -0.8746519899888767 1101 10 -9.68231282828603 1101 11 -4.917917444322963 1101 12 -2.479615461314534 1101 13 -1.4790356373627596 1101 14 -1.4892684905448839 1101 15 -2.913154468692703 1101 16 -1.3283135679701132 1101 17 -1.255105140083451 1101 18 -1.125916994915002 1101 19 -1.4493762283286564 1101 20 -0.8813152240620781 1101 21 -1.01413881752638 1101 22 -0.6031467581842449 1101 23 -0.7665320526960997 1101 24 -1.0599773989263106 1101 25 -0.8729500863730699 1102 1 -0.5163184897756778 1102 2 -0.694957826342133 1102 3 -0.9131930375647315 1102 4 -0.7570717599782171 1102 5 -0.5646987934818419 1102 6 -1.702283153554344 1102 7 -0.4158027214179256 1102 8 -0.1305313287056471 1102 9 -0.26115963559783495 1102 10 -0.19944439158096117 1102 11 -0.4404833488795994 1102 12 -0.3833688001641491 1102 13 -0.41310364564157 1102 14 -0.7404575270467149 1102 15 -0.3630920264435531 1102 16 -0.35527457284039343 1102 17 -0.5289687240710208 1102 18 -0.6356674234510038 1102 19 -0.04092929546120416 1102 20 -0.5073091971230212 1102 21 -0.7116330067757772 1102 22 -0.5556313890506885 1102 23 -1.097666921369843 1102 24 -4.321209870651714 1102 25 -2.7412485186226796 1103 1 -0.2217273002718123 1103 2 0.022619721663772907 1103 3 0.08268627427817421 1103 4 0.07207933916158638 1103 5 -0.18850842073008067 1103 6 0.7554499249059923 1103 7 0.3830916221392897 1103 8 0.8644269648037799 1103 9 0.823634873047574 1103 10 0.7740524369879864 1103 11 0.3980973271130992 1103 12 0.0689554678000519 1103 13 0.29318925556617614 1103 14 0.2646341403440218 1103 15 0.5113844702112402 1103 16 0.543830475382741 1103 17 0.087600917079385 1103 18 0.03999944595554439 1103 19 -1.5256151934540831 1103 20 -0.43814810580318664 1103 21 -0.05767740681053368 1103 22 0.087358879434845 1103 23 -0.2625791639625987 1103 24 1.579367938019761 1103 25 0.7954814318508596 1104 1 -0.70295693498284 1104 2 -0.5085756136131927 1104 3 -1.6426481452039035 1104 4 -0.711199686734485 1104 5 -0.6573688220334617 1104 6 -1.9868670018647165 1104 7 -0.5444551108396141 1104 8 -0.10179238007702907 1104 9 -0.06585235041324111 1104 10 -0.2870048430668959 1104 11 -0.1974737584639854 1104 12 0.037537186201696215 1104 13 -0.5416203825747914 1104 14 -0.7180274957389798 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1108 24 0.1665676066195978 1108 25 0.3865543398873186 1109 1 0.4770305134803934 1109 2 0.4059984682577813 1109 3 -0.8658351233817072 1109 4 0.19610562542541032 1109 5 0.7968538168079853 1109 6 0.521029344684802 1109 7 0.9611930807063693 1109 8 1.3788162900161538 1109 9 0.6033431596319616 1109 10 0.6378567325481725 1109 11 0.18957432663225404 1109 12 0.08533629806131415 1109 13 0.21537630477278213 1109 14 0.25379907062131224 1109 15 -1.1134512406398578 1109 16 -0.016804241719363233 1109 17 -0.008424086927668384 1109 18 -0.008125253515522957 1109 19 -1.490713766943422 1109 20 -0.41798862511717516 1109 21 -0.1297442444447725 1109 22 0.30463328238403875 1109 23 0.25292412182037793 1109 24 -0.4772517125652662 1109 25 -0.2568466656006514 1110 1 1.0493511311007389 1110 2 0.9309530549971079 1110 3 1.2708398690494178 1110 4 1.3810662672661635 1110 5 1.289841252472531 1110 6 1.3922146307883452 1110 7 1.4405262626621678 1110 8 1.922515200097898 1110 9 1.6367067684724077 1110 10 1.3320589729356982 1110 11 1.057247653910487 1110 12 1.3101319383882881 1110 13 1.2855095170040869 1110 14 1.1775426370760513 1110 15 1.601580548642609 1110 16 1.2974252466245701 1110 17 0.9726499366872261 1110 18 1.0724340864784239 1110 19 -1.072510618290682 1110 20 0.6886492798177315 1110 21 0.5960807631241067 1110 22 1.0318850232256978 1110 23 1.0458358687171412 1110 24 -0.12357323845082746 1110 25 0.6119846998247169 1111 1 0.4343226410722494 1111 2 0.2978333968774507 1111 3 0.8479410086505581 1111 4 0.6368354980353921 1111 5 0.846144671275951 1111 6 0.757100670674383 1111 7 1.121331105967009 1111 8 1.2010408411235396 1111 9 0.49739052915617066 1111 10 0.4982982891750112 1111 11 0.12783169949453566 1111 12 0.2916134198697691 1111 13 0.22411350560807364 1111 14 0.42077625530966856 1111 15 -0.6014109043488389 1111 16 -0.5339137847144642 1111 17 -0.04811217157487846 1111 18 0.22631585286018918 1111 19 0.6097543389742768 1111 20 0.06521623138468158 1111 21 0.35590982295316564 1111 22 0.2629035417442154 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0.9136536557049304 2215 2 0.08475065876261922 2215 3 0.4690016861939057 2215 4 0.7794794750824501 2215 5 0.9199243361981075 2215 6 1.3667653368217216 2215 7 1.1097906329374467 2215 8 -0.056801231224309134 2215 9 0.2118173090514972 2215 10 0.1269313163959751 2215 11 -0.22869229759429288 2215 12 -0.2758757637939979 2215 13 0.26403611805957183 2215 14 0.24762893004424313 2215 15 0.12458012074359459 2215 16 0.3845312229474871 2215 17 0.11299546514251091 2215 18 -0.492640439266836 2215 19 0.657481536894025 2215 20 -2.9013985022390365 2215 21 -1.3092537112732678 2215 22 -0.9279869636520672 2215 23 -0.6814551739301251 2215 24 -0.5774885144456903 2215 25 -0.43162534153885446 2216 1 0.49336154776740315 2216 2 -0.5648802783362257 2216 3 0.2816905634938607 2216 4 -0.09610387162272715 2216 5 0.048869030250308754 2216 6 -1.4377332858783234 2216 7 -9.657303598448944 2216 8 -10.77071396656877 2216 9 -5.539768925972037 2216 10 -3.320111165953193 2216 11 -2.044488457200625 2216 12 -1.632576394945044 2216 13 -1.3473897885514674 2216 14 -1.441815001812838 2216 15 -2.0585757013573875 2216 16 -1.3081303089309877 2216 17 -1.4437563133127718 2216 18 -1.2983124412993554 2216 19 -0.9564515905129056 2216 20 -1.661524331293382 2216 21 -4.5246657646536725 2216 22 -2.8606496989965287 2216 23 -2.994702325727303 2216 24 -2.769666988450826 2216 25 -2.405376411286394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35381.0 3 2.0 4 289.0 5 1552.0 6 2006.0 7 1634.0 8 1269.0 9 1215.0 10 1140.0 11 1094.0 12 1085.0 13 1015.0 14 975.0 15 1029.0 16 1060.0 17 1172.0 18 1319.0 19 1682.0 20 2199.0 21 2770.0 22 3433.0 23 4174.0 24 4688.0 25 5211.0 26 6100.0 27 6669.0 28 7271.0 29 8578.0 30 10070.0 31 12079.0 32 15467.0 33 20053.0 34 26446.0 35 36520.0 36 51966.0 37 81387.0 38 97425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47042068361087 18.65096406660824 12.813102541630151 25.065512708150745 2 13.953493462685978 19.9939147051806 43.18808157797386 22.864510254159562 3 19.54025756259016 24.266397560532464 29.406266349401626 26.78707852747575 4 10.938942486491216 16.70648202839579 39.669882522806326 32.68469296230666 5 12.456622562171594 38.63855524432404 36.265615330958646 12.639206862545729 6 31.29132701255991 37.7166934891369 18.88688206276458 12.105097435538607 7 27.748975850241674 31.42516908339693 22.604322920778618 18.221532145582778 8 24.02072797926325 38.06913127574002 21.05451291880299 16.855627826193746 9 25.68341139376129 16.31244287594733 20.52420747893243 37.47993825135895 10 13.33245858618993 31.039746323328416 35.41020173855995 20.21759335192171 11 34.064139202228496 23.755162906226975 23.803922168846984 18.37677572269755 12 21.93148747012662 26.347390493432776 32.44188766953757 19.27923436690303 13 28.270941701486596 22.8683314638802 25.94188733866401 22.918839495969195 14 19.40014256289604 23.53228000052477 29.017234367278753 28.05034306930044 15 22.878002064055693 31.877416082142418 23.448022529692665 21.796559324109218 16 20.37893862200162 30.26436052740909 28.955458859029587 20.401241991559704 17 20.393798924213932 29.945117418113437 28.486465211877377 21.17461844579525 18 19.71413703230772 29.740331298430966 30.406120449894175 20.13941121936714 19 22.385716316244082 28.25291654366342 28.5931708597107 20.7681962803818 20 21.82650658258681 28.8184141880383 29.64274078754895 19.71233844182594 21 22.00847926428498 28.081495394128357 28.488403873611297 21.421621467975363 22 20.90447734433592 29.596564534150215 29.09147758704004 20.407480534473823 23 20.828349838086513 28.888383554575015 28.912654178592433 21.37061242874604 24 21.54022420514749 28.242764278483172 29.73024976549637 20.486761750872965 25 21.179621733901826 28.650049196457854 29.23253525746146 20.937793812178857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 149.0 1 149.0 2 208.0 3 267.0 4 267.0 5 267.0 6 451.0 7 635.0 8 635.0 9 635.0 10 976.0 11 1317.0 12 1317.0 13 1317.0 14 2133.5 15 2950.0 16 2950.0 17 2950.0 18 4851.0 19 6752.0 20 6752.0 21 6752.0 22 10237.5 23 13723.0 24 13723.0 25 13723.0 26 18827.5 27 23932.0 28 23932.0 29 23932.0 30 30472.5 31 37013.0 32 37013.0 33 37013.0 34 42798.0 35 48583.0 36 48583.0 37 48583.0 38 52658.5 39 56734.0 40 56734.0 41 56734.0 42 59009.5 43 61285.0 44 61285.0 45 61285.0 46 61980.5 47 62676.0 48 62676.0 49 62676.0 50 58891.0 51 55106.0 52 55106.0 53 55106.0 54 46921.0 55 38736.0 56 38736.0 57 38736.0 58 31631.0 59 24526.0 60 24526.0 61 24526.0 62 18814.0 63 13102.0 64 13102.0 65 13102.0 66 9604.0 67 6106.0 68 6106.0 69 6106.0 70 4294.0 71 2482.0 72 2482.0 73 2482.0 74 1688.5 75 895.0 76 895.0 77 895.0 78 590.0 79 285.0 80 285.0 81 285.0 82 195.5 83 106.0 84 106.0 85 106.0 86 73.5 87 41.0 88 41.0 89 41.0 90 27.5 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 5.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22408045034705143 2 0.12810843307646064 3 0.13204350439962836 4 0.06886374815543532 5 0.022298737497950485 6 0.021205662130403893 7 0.31414986063289063 8 0.31108924960376017 9 0.019019511395310707 10 0.03279226102639777 11 0.016614745586708203 12 0.01705197573372684 13 0.01574028529267093 14 0.018145051101273433 15 0.015958900366180247 16 0.020768431983385256 17 0.01858228124829207 18 0.015958900366180247 19 0.026233808821118216 20 0.017926436027764114 21 0.016177515439689566 22 0.01705197573372684 23 0.017926436027764114 24 0.016614745586708203 25 0.016396130513198885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 457425.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.30935730039538 #Duplication Level Percentage of deduplicated Percentage of total 1 96.54847886967059 88.15779553920424 2 2.6871250954255 4.907193308981319 3 0.332346938807448 0.910391561497857 4 0.12922846809245708 0.47199073465747626 5 0.07074843709332425 0.3229997160499445 6 0.04740250536150498 0.25969753793925393 7 0.028196064071545946 0.18021951421415405 8 0.021014499252745393 0.15350563366062564 9 0.01586569917919519 0.1303818115656351 >10 0.09994060163167347 1.755008452630117 >50 0.011264423429421614 0.7035700510260703 >100 0.007190055415386538 1.3585573520323702 >500 0.001198342569231075 0.688688786540889 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 909 0.1987211018199705 No Hit GGTATCAACGCAGAGTACTTTTTTT 621 0.13575996064928678 No Hit GTCCTACAGTGGACATTTCTAAATT 592 0.12942012351751653 No Hit GTCCTACAGTGTGCATTTCTCATTT 521 0.11389845329835492 No Hit CACACACACACACACACACACACAC 504 0.11018199704869651 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 493 0.107777231240094 No Hit CTGTAGGACGTGGAATATGGCAAGA 487 0.1064655407990381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 2.1861507350931847E-4 0.0 11 0.0 0.0 0.0 2.1861507350931847E-4 0.0 12 0.0 0.0 0.0 2.1861507350931847E-4 0.0 13 0.0 0.0 0.0 2.1861507350931847E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAGGG 35 0.0021158154 16.344126 3 GTGTAGG 85 1.6039849E-8 14.5975685 1 GGACTAG 40 0.0052119466 14.274437 6 TGTACTG 95 4.7584763E-9 14.02247 5 GACGTGG 120 4.9112714E-11 13.481413 7 AGGACGT 170 0.0 13.433291 5 GCACTGT 50 0.0014775117 13.322808 6 ATAAGAC 65 5.2563882E-5 13.201025 3 GTATAAG 90 5.0801464E-7 12.726086 1 CTAGACT 60 4.0161482E-4 12.691174 4 TAGGACG 195 0.0 12.691174 4 GGACGTG 180 0.0 12.688389 6 GGCGAGG 85 4.0055565E-6 12.272119 19 TTAGAAC 55 0.0029816732 12.1342745 3 TCTAACA 55 0.0029816732 12.1342745 2 TTACTCA 55 0.0030187075 12.114302 4 GGTGTGC 80 2.8284632E-5 11.886236 8 CGTGGAA 145 1.0004442E-10 11.772 9 CTAGACA 65 7.8755914E-4 11.71493 4 TATACTG 115 6.8694135E-8 11.583779 5 >>END_MODULE