FastQCFastQC Report
Thu 26 May 2016
SRR1926975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457425
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15670.34256982018910204No Hit
GGTATCAACGCAGAGTACTTTTTTT10960.23960212056621305No Hit
TATCAACGCAGAGTACTTTTTTTTT7790.1703011422637591No Hit
CACACACACACACACACACACACAC7140.15609116248565338No Hit
GTCCTACAGTGGACATTTCTAAATT6320.13816472645788927No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6090.13313657976717497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5800.12679674263540472No Hit
GTCCTACAGTGTGCATTTCTCATTT5780.1263595124883861No Hit
CTGTAGGACGTGGAATATGGCAAGA5590.12220582609170903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG307.549056E-419.060162
CTAGACA359.7806005E-519.0350824
TAGGACC1900.014.5267744
TAGACTG1051.3224053E-913.596495
GGTCTAC603.974732E-412.7067731
CTATACT1001.3996942E-712.3728054
CTAGGAC853.8459402E-612.3168193
GTAGGAC3650.012.25546553
GTATTAG550.002991053512.1291931
CCTATAC550.003020705712.1132363
GACCATC550.00308076712.0814467
GACAGTG951.0442709E-611.9906077
TGTAGGA4400.011.91260052
TAAGCTG657.881436E-411.7138985
TGGCGAG3450.011.55237618
TTACAAT1157.133349E-811.55237616
CTGTAGG3750.011.4360961
GTGTAAC1001.847915E-611.4360961
TTAGGAC1850.011.3181583
TATACTG1104.8218135E-711.2480055
Produced by FastQC (version 0.11.5)