##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926975_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 457425 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.859782478001858 31.0 30.0 33.0 26.0 34.0 2 30.137519811991037 31.0 30.0 34.0 26.0 34.0 3 30.188343444280484 31.0 30.0 34.0 26.0 34.0 4 33.78138274033994 35.0 35.0 37.0 28.0 37.0 5 33.76021424277204 37.0 35.0 37.0 28.0 37.0 6 33.71846094988249 37.0 35.0 37.0 29.0 37.0 7 33.717724217084765 37.0 35.0 37.0 29.0 37.0 8 33.73993113625185 37.0 35.0 37.0 29.0 37.0 9 35.24943105427119 39.0 35.0 39.0 29.0 39.0 10 35.15600371645625 39.0 35.0 39.0 29.0 39.0 11 35.14450893589113 39.0 35.0 39.0 29.0 39.0 12 35.04045253320216 39.0 35.0 39.0 28.0 39.0 13 35.04280264524239 39.0 35.0 39.0 29.0 39.0 14 36.145523309832214 40.0 36.0 41.0 28.0 41.0 15 36.13102257200634 40.0 36.0 41.0 28.0 41.0 16 36.120045909165434 40.0 36.0 41.0 28.0 41.0 17 36.11767174946713 40.0 36.0 41.0 28.0 41.0 18 36.08414931409521 40.0 36.0 41.0 28.0 41.0 19 36.17605727714926 40.0 37.0 41.0 28.0 41.0 20 36.13054599114609 40.0 37.0 41.0 28.0 41.0 21 36.08085915723889 40.0 37.0 41.0 28.0 41.0 22 35.996799475323826 40.0 36.0 41.0 27.0 41.0 23 35.92797945018309 40.0 36.0 41.0 27.0 41.0 24 35.861258129748045 40.0 36.0 41.0 27.0 41.0 25 35.745927747718206 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.39074253490687383 1101 2 -0.37315801255632053 1101 3 -0.394465422747583 1101 4 -0.4010328686062081 1101 5 -0.31948002629616923 1101 6 -0.38126860186867617 1101 7 -0.16561835236384326 1101 8 -0.1004104654988538 1101 9 -0.11283819846865839 1101 10 -0.15336829777682226 1101 11 -0.15887937938150998 1101 12 -0.19727181914812064 1101 13 0.051204764403408376 1101 14 -0.12891547428679928 1101 15 -0.0311413594689256 1101 16 0.10954181954899411 1101 17 -0.0595891463120779 1101 18 -0.14248925362572606 1101 19 -0.09889210354679534 1101 20 -0.028983780490769107 1101 21 -0.09436381595888577 1101 22 -0.16982372448798344 1101 23 -0.22927559543085607 1101 24 -0.019079040355521215 1101 25 -0.2976386842996277 1102 1 -0.7419906117075463 1102 2 -0.627266141900666 1102 3 -0.5985275708926423 1102 4 -0.8167380270602749 1102 5 -0.8051622051246241 1102 6 -0.8277295858882923 1102 7 -0.6621933565888511 1102 8 -0.6212249143499804 1102 9 -0.4665861600419845 1102 10 -0.48847796129101795 1102 11 -0.6283529111107313 1102 12 -0.6496456122788103 1102 13 -0.6893954274226957 1102 14 -0.6487598185992809 1102 15 -0.6887690807548168 1102 16 -0.8900609204476311 1102 17 -0.8735968413041846 1102 18 -0.7564820239326195 1102 19 -0.6339288994802601 1102 20 -0.6747771043279087 1102 21 -0.4928748591285341 1102 22 -0.551393055137801 1102 23 -0.41207006715187333 1102 24 -0.7122209280209262 1102 25 -0.5201415979054573 1103 1 -0.2983030628327512 1103 2 -0.24272360996913633 1103 3 -0.03051340806355185 1103 4 0.1658690630431181 1103 5 -0.30207114595354057 1103 6 -0.4521430883646147 1103 7 -0.4300554645043917 1103 8 -0.3261971100229317 1103 9 -0.7390924594598332 1103 10 -0.6162670828480543 1103 11 -0.44815697749356076 1103 12 -0.37330101240045366 1103 13 -0.20273064334067215 1103 14 -0.42139427858712253 1103 15 -0.3037508341651929 1103 16 -0.2835160425260028 1103 17 -0.3083327001972265 1103 18 -0.18135386336263082 1103 19 -0.3803404035205773 1103 20 -0.1471508987496648 1103 21 -0.25118140183424487 1103 22 -0.15925646638117286 1103 23 -0.2525340518594916 1103 24 -0.22451999831373826 1103 25 -0.14743328267389444 1104 1 -0.053765247706703434 1104 2 0.05563001719601601 1104 3 0.2903192106982395 1104 4 0.4070498210606104 1104 5 0.2521348221479869 1104 6 0.1171632055815337 1104 7 0.46107384436201215 1104 8 0.37127759414445194 1104 9 0.3746487513101897 1104 10 0.39244068597376014 1104 11 0.4089644425030059 1104 12 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2215 15 0.1361627050630787 2215 16 0.00508359501836253 2215 17 0.08777278949543899 2215 18 -0.054347142831034034 2215 19 -0.23474272927757767 2215 20 0.1836540548107095 2215 21 0.4659861583862508 2215 22 0.31407283966730404 2215 23 0.2714635317695979 2215 24 0.4028567290139051 2215 25 0.2449627001094612 2216 1 -0.314739950087656 2216 2 0.03570776257988584 2216 3 -1.6767756629655146 2216 4 -1.0885580202902148 2216 5 -0.26598045967902806 2216 6 -0.1401026471881437 2216 7 0.009114265887767203 2216 8 -0.006068433552343322 2216 9 -0.233378611420612 2216 10 0.11499823577939594 2216 11 0.005121208780948905 2216 12 -0.2446092231847672 2216 13 -0.15735686883086686 2216 14 -0.22975824917535448 2216 15 -0.532549853773034 2216 16 -0.2385865082122791 2216 17 -0.4345581903933038 2216 18 -0.6545156280685163 2216 19 -0.5718385652852831 2216 20 -0.6524205065927973 2216 21 -0.47595162242247824 2216 22 -0.5290787703027462 2216 23 -0.3794795175457679 2216 24 -0.46094769439216776 2216 25 -0.2401109787523268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11020.0 3 8.0 4 172.0 5 1288.0 6 1774.0 7 1330.0 8 1017.0 9 923.0 10 770.0 11 753.0 12 631.0 13 614.0 14 611.0 15 598.0 16 603.0 17 680.0 18 691.0 19 843.0 20 1101.0 21 1423.0 22 1780.0 23 2210.0 24 2586.0 25 3052.0 26 3678.0 27 4048.0 28 4647.0 29 5709.0 30 7420.0 31 9448.0 32 12214.0 33 15789.0 34 21499.0 35 30743.0 36 44686.0 37 77206.0 38 183860.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.051319089703355 19.631390264080576 10.777052610383755 27.540238035832314 2 13.822333992133792 21.03411973534169 41.124469512422 24.01907676010252 3 18.873564022617053 24.629717899045378 28.843302999427138 27.653415078910427 4 11.198775755588347 17.72880800131169 38.73443733945455 32.33797890364541 5 12.10789586448741 39.09869611984154 35.73012041871081 13.063287596960235 6 30.719407260532073 38.97156719315139 18.096495634073744 12.212529912242799 7 28.04911913971535 32.301125865608945 20.997863719751518 18.651891274924182 8 24.477351535478004 39.20639640388122 19.60877341040968 16.707478650231096 9 25.805076315265897 16.392094958448027 20.10564542753384 37.69718329875224 10 13.463661624497988 31.512716190542818 34.62787133615648 20.395750848802713 11 33.98752967822897 23.912235519429128 23.418582178633425 18.681652623708477 12 21.91909895217337 26.621966975862605 32.0499843689539 19.408949703010126 13 28.27804521863837 22.862914332935453 25.85708601685096 23.001954431575218 14 19.43863898702298 23.729625030606176 28.514026373780126 28.317709608590718 15 22.302164564627592 32.05636918933218 23.640123914274408 22.001342331765823 16 20.567396042612636 30.731292479827204 28.39800359841984 20.303307879140313 17 20.314850117177937 30.040837385008217 28.264148798488932 21.380163699324914 18 19.652530923304955 29.72725404549847 30.35818128555251 20.262033745644068 19 21.742675816476215 28.295254064618526 28.95350692903505 21.00856318987021 20 21.631884780355996 28.894981002225517 29.613792078212242 19.859342139206245 21 21.802984056462687 28.01575987615991 28.536351789821673 21.64490427755573 22 20.839919112422802 29.66956331639066 28.96584139476417 20.524676176422364 23 20.833624820735245 28.845228934205462 28.91299975515058 21.408146489908706 24 20.695241176676344 28.483857427681976 30.182281646787224 20.638619748854452 25 21.10443558260748 28.522580193794383 29.438862070171755 20.934122153426383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 231.0 2 227.0 3 223.0 4 223.0 5 223.0 6 399.5 7 576.0 8 576.0 9 576.0 10 902.0 11 1228.0 12 1228.0 13 1228.0 14 1953.0 15 2678.0 16 2678.0 17 2678.0 18 4456.5 19 6235.0 20 6235.0 21 6235.0 22 9654.0 23 13073.0 24 13073.0 25 13073.0 26 18323.5 27 23574.0 28 23574.0 29 23574.0 30 30365.0 31 37156.0 32 37156.0 33 37156.0 34 42560.5 35 47965.0 36 47965.0 37 47965.0 38 52066.0 39 56167.0 40 56167.0 41 56167.0 42 58646.5 43 61126.0 44 61126.0 45 61126.0 46 62340.0 47 63554.0 48 63554.0 49 63554.0 50 60317.5 51 57081.0 52 57081.0 53 57081.0 54 48155.0 55 39229.0 56 39229.0 57 39229.0 58 31931.5 59 24634.0 60 24634.0 61 24634.0 62 18865.5 63 13097.0 64 13097.0 65 13097.0 66 9575.5 67 6054.0 68 6054.0 69 6054.0 70 4229.0 71 2404.0 72 2404.0 73 2404.0 74 1606.5 75 809.0 76 809.0 77 809.0 78 524.5 79 240.0 80 240.0 81 240.0 82 152.0 83 64.0 84 64.0 85 64.0 86 42.0 87 20.0 88 20.0 89 20.0 90 13.0 91 6.0 92 6.0 93 6.0 94 3.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11346122315133629 2 0.11674044925397606 3 0.015521670219161612 4 0.0 5 0.004590916543695688 6 0.32967153085205225 7 0.019238126468820026 8 0.009400448160900694 9 0.01005629338142865 10 0.0034978411761490955 11 0.004153686396677051 12 4.3723014701863694E-4 13 6.558452205279554E-4 14 2.1861507350931847E-4 15 0.0026233808821118218 16 4.3723014701863694E-4 17 2.1861507350931847E-4 18 6.558452205279554E-4 19 4.3723014701863694E-4 20 6.558452205279554E-4 21 0.013335519484068427 22 0.0 23 2.1861507350931847E-4 24 2.1861507350931847E-4 25 0.00721429742580751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 457425.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.90582378381194 #Duplication Level Percentage of deduplicated Percentage of total 1 95.4010911244199 84.81712596291061 2 3.650325890544716 6.490704607565097 3 0.4217685544194253 1.1249304233029955 4 0.15938873422835614 0.5668234687372427 5 0.08167847872853579 0.3630846218384517 6 0.057854567713068863 0.3086164801312029 7 0.03691126550230656 0.22971385264698949 8 0.028715844098037648 0.20424046201468413 9 0.017033173117544435 0.13629134589008113 >10 0.1220914625650257 2.0985547168729166 >50 0.012555172436174485 0.7730425492235314 >100 0.008370113853781426 1.329843160566408 >500 0.0017232587346020024 0.9741823416196458 >1k 4.92359638457715E-4 0.5828460066800981 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1567 0.34256982018910204 No Hit GGTATCAACGCAGAGTACTTTTTTT 1096 0.23960212056621305 No Hit TATCAACGCAGAGTACTTTTTTTTT 779 0.1703011422637591 No Hit CACACACACACACACACACACACAC 714 0.15609116248565338 No Hit GTCCTACAGTGGACATTTCTAAATT 632 0.13816472645788927 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 609 0.13313657976717497 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 580 0.12679674263540472 No Hit GTCCTACAGTGTGCATTTCTCATTT 578 0.1263595124883861 No Hit CTGTAGGACGTGGAATATGGCAAGA 559 0.12220582609170903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.1861507350931847E-4 0.0 8 0.0 0.0 0.0 2.1861507350931847E-4 0.0 9 0.0 0.0 0.0 2.1861507350931847E-4 0.0 10 0.0 0.0 0.0 2.1861507350931847E-4 0.0 11 0.0 0.0 0.0 2.1861507350931847E-4 2.1861507350931847E-4 12 0.0 0.0 0.0 2.1861507350931847E-4 2.1861507350931847E-4 13 0.0 0.0 0.0 2.1861507350931847E-4 2.1861507350931847E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAAGG 30 7.549056E-4 19.06016 2 CTAGACA 35 9.7806005E-5 19.035082 4 TAGGACC 190 0.0 14.526774 4 TAGACTG 105 1.3224053E-9 13.59649 5 GGTCTAC 60 3.974732E-4 12.706773 1 CTATACT 100 1.3996942E-7 12.372805 4 CTAGGAC 85 3.8459402E-6 12.316819 3 GTAGGAC 365 0.0 12.2554655 3 GTATTAG 55 0.0029910535 12.129193 1 CCTATAC 55 0.0030207057 12.113236 3 GACCATC 55 0.003080767 12.081446 7 GACAGTG 95 1.0442709E-6 11.990607 7 TGTAGGA 440 0.0 11.9126005 2 TAAGCTG 65 7.881436E-4 11.713898 5 TGGCGAG 345 0.0 11.552376 18 TTACAAT 115 7.133349E-8 11.552376 16 CTGTAGG 375 0.0 11.436096 1 GTGTAAC 100 1.847915E-6 11.436096 1 TTAGGAC 185 0.0 11.318158 3 TATACTG 110 4.8218135E-7 11.248005 5 >>END_MODULE