FastQCFastQC Report
Thu 26 May 2016
SRR1926974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474015
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9460.19957174351022647No Hit
GTCCTACAGTGGACATTTCTAAATT7880.16623946499583347No Hit
CTGTAGGACGTGGAATATGGCAAGA7130.1504171808909001No Hit
GTCCTACAGTGTGCATTTCTCATTT6750.14240055694440049No Hit
GGTATCAACGCAGAGTACTTTTTTT5930.12510152632300664No Hit
GTCCTAAAGTGTGTATTTCTCATTT5120.10801345948967858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGT307.4830395E-419.0852621
CCGTGAT350.002191400616.25866719
CCAGTAG456.5720844E-414.8299223
TCCAACG653.418636E-614.59111318
CTAACAC602.4771185E-514.3002813
CGGTTTC400.005330390314.22633513
CCGGTTT400.005330390314.22633512
TGCCGGT400.005330390314.22633510
TAGGACC1650.013.8434434
CTAGGAC852.572815E-713.4590873
TCTGGAC500.001457635113.346933
AAGACTG655.3604075E-513.1737085
GATATAC905.0939525E-712.7235081
GACGTGG1650.012.6857937
GTAGGAC3550.012.6218443
GTCCTAA1457.2759576E-1212.5041381
ACGTGGA1600.012.4756918
TAGGACA1301.891749E-1012.4457874
TGGACAC853.8612397E-612.312685
CTCCTAT701.0432555E-412.2690971
Produced by FastQC (version 0.11.5)