##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926974_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 474015 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.617855975021886 30.0 27.0 31.0 10.0 31.0 2 27.26119004672848 31.0 28.0 31.0 10.0 34.0 3 27.055880088182864 31.0 27.0 31.0 10.0 34.0 4 30.656934907123194 35.0 31.0 37.0 10.0 37.0 5 30.4597808086242 35.0 30.0 37.0 10.0 37.0 6 30.118255751400273 35.0 30.0 37.0 10.0 37.0 7 30.187572123245044 35.0 30.0 37.0 10.0 37.0 8 29.84044386781009 35.0 30.0 37.0 8.0 37.0 9 31.9018596457918 37.0 32.0 39.0 10.0 39.0 10 32.03824773477633 37.0 32.0 39.0 8.0 39.0 11 32.21435186650211 37.0 32.0 39.0 8.0 39.0 12 32.06210773920657 37.0 32.0 39.0 2.0 39.0 13 32.18071580013291 37.0 32.0 39.0 2.0 39.0 14 33.091355758784005 38.0 32.0 40.0 2.0 41.0 15 32.65173675938525 38.0 32.0 40.0 2.0 41.0 16 32.72436315306478 38.0 32.0 40.0 2.0 41.0 17 32.95036865921964 38.0 32.0 40.0 2.0 41.0 18 32.99611615666171 38.0 32.0 40.0 2.0 41.0 19 32.36861491724945 38.0 32.0 40.0 2.0 41.0 20 32.65798972606352 39.0 32.0 40.0 2.0 41.0 21 32.647454194487516 39.0 32.0 40.0 2.0 41.0 22 32.653179751695625 39.0 32.0 40.0 2.0 41.0 23 32.62898853411812 39.0 32.0 40.0 2.0 41.0 24 31.307226564560192 38.0 30.0 40.0 2.0 41.0 25 31.86145375146356 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5423485948177174 1101 2 -0.7187128495881403 1101 3 -1.5896129050584165 1101 4 -0.93352096972977 1101 5 -0.5363590622658165 1101 6 -3.361398559425659 1101 7 -1.2033721747556712 1101 8 -0.3382876013371572 1101 9 -0.5708858361450488 1101 10 -9.398710206606637 1101 11 -4.188460466953835 1101 12 -2.144122765023109 1101 13 -1.0337545439819849 1101 14 -1.0515806073705178 1101 15 -2.418602348099011 1101 16 -0.9334922665229755 1101 17 -0.5207665511049413 1101 18 -0.4190752875393997 1101 19 -1.4874294550065628 1101 20 -0.7444961202209655 1101 21 -0.9019840693496342 1101 22 -0.5292854161346767 1101 23 -0.5940555910955752 1101 24 -0.9518378234722498 1101 25 -0.7850345604340774 1102 1 -0.8090044771474467 1102 2 -0.4595415601233839 1102 3 -0.5630755452219169 1102 4 -0.39757756382573817 1102 5 -0.3346143669759414 1102 6 -0.9779285806523887 1102 7 -0.13583329196037397 1102 8 0.3441163062752679 1102 9 0.19124457016142316 1102 10 -0.03690490860384088 1102 11 -0.0015231923399028346 1102 12 0.060592883575850465 1102 13 -0.3743962383328352 1102 14 -0.11456245022957745 1102 15 0.34674363184212353 1102 16 -0.049174007588412394 1102 17 -0.288901991855532 1102 18 -0.43229662158818627 1102 19 0.0276383679514538 1102 20 0.2586247616747599 1102 21 0.0499677641191596 1102 22 0.11517866807784571 1102 23 -0.25087300174998006 1102 24 -3.420175273265901 1102 25 -1.5927669300159337 1103 1 -0.8100776172639037 1103 2 -1.0196974560016763 1103 3 -0.5052712596349238 1103 4 -0.9611640213398509 1103 5 -1.692121117685879 1103 6 -0.5803011113647294 1103 7 -0.8187630032198214 1103 8 -0.6547505042515773 1103 9 -0.8756232941347797 1103 10 -1.0938781885771895 1103 11 -1.361980109832082 1103 12 -1.4875608892671188 1103 13 -1.5980277909767473 1103 14 -1.3453766807648684 1103 15 -1.1872927309489718 1103 16 -1.2016625691545038 1103 17 -1.567912610906287 1103 18 -1.8822658220794253 1103 19 -3.132811576067052 1103 20 -2.115366505986529 1103 21 -2.0079740538654285 1103 22 -1.8942103394031449 1103 23 -1.813310773182753 1103 24 -0.40611016426663227 1103 25 -0.947803908797205 1104 1 -0.6163556321592694 1104 2 -0.29828819317484445 1104 3 -1.3621793526037216 1104 4 -0.5020727035421295 1104 5 -0.7382267243954885 1104 6 -1.6161027845582403 1104 7 -0.34111274389969637 1104 8 -0.05707644602076911 1104 9 0.16414422647404336 1104 10 0.0158129803816891 1104 11 0.14219522156392372 1104 12 -0.07847598034004122 1104 13 -0.060943387106938474 1104 14 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19 0.4222140002778403 2213 20 -1.373308847207177 2213 21 -0.24797426977548298 2213 22 -0.3625552269229999 2213 23 -0.3098661082301746 2213 24 -0.6864045936073673 2213 25 -0.3662951653397286 2214 1 1.0075673672795489 2214 2 0.4061299211469809 2214 3 0.4949882886079351 2214 4 0.75184185161039 2214 5 0.825239079785586 2214 6 0.46963239058546336 2214 7 1.071938546730685 2214 8 -2.099807721214219 2214 9 -0.997431969490048 2214 10 -0.5026237113699992 2214 11 -0.15331585248910784 2214 12 -1.087709816258883 2214 13 -0.38298580681098215 2214 14 -0.1433532784633016 2214 15 -1.6151052046585264 2214 16 -0.40530949046484466 2214 17 -0.09187428368576178 2214 18 0.05529224065249139 2214 19 0.7091736636928729 2214 20 -1.0220490677455842 2214 21 -0.1842019408026374 2214 22 0.05817598294145654 2214 23 -0.31415833244477653 2214 24 -1.8453284407589017 2214 25 -0.7124885218823742 2215 1 1.0621717947653337 2215 2 0.17819981186094225 2215 3 0.5875147879140954 2215 4 0.4958846465006417 2215 5 0.9896094475457815 2215 6 1.4090510418685191 2215 7 1.059347076512097 2215 8 -0.27919086669905724 2215 9 0.2892605714397831 2215 10 -0.05675579092580563 2215 11 7.484371684043367E-4 2215 12 -0.3722696601407627 2215 13 0.12265300391257483 2215 14 -0.0347921190546856 2215 15 -0.04391490465466319 2215 16 0.2828670451693185 2215 17 0.13114609187698534 2215 18 -0.7072182676207319 2215 19 0.31080397718759656 2215 20 -3.1782544442899443 2215 21 -1.8161967592381316 2215 22 -0.8871474369582373 2215 23 -0.6449344428819046 2215 24 -0.6867621635485541 2215 25 -0.6296741000849018 2216 1 0.11041169046285404 2216 2 -0.6796880629760871 2216 3 -0.14521011430233344 2216 4 -0.26943477983573416 2216 5 -0.12903462025082746 2216 6 -1.916894199330958 2216 7 -9.702061006929885 2216 8 -10.860677176998927 2216 9 -5.934989754505462 2216 10 -3.3501065080053394 2216 11 -2.3069438705239023 2216 12 -1.8611875219399785 2216 13 -1.9972948448488346 2216 14 -2.016539185547515 2216 15 -3.309886221473441 2216 16 -1.9778891477902647 2216 17 -2.3460377412390514 2216 18 -1.995353860841071 2216 19 -1.5379574308958475 2216 20 -2.3633913412912477 2216 21 -4.957005103436305 2216 22 -3.798490331351978 2216 23 -3.6097323568323603 2216 24 -3.565510533822348 2216 25 -3.1968188197719947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37349.0 3 5.0 4 304.0 5 1686.0 6 2019.0 7 1676.0 8 1317.0 9 1242.0 10 1188.0 11 1111.0 12 1094.0 13 1097.0 14 1079.0 15 1117.0 16 1136.0 17 1249.0 18 1441.0 19 1770.0 20 2373.0 21 2920.0 22 3725.0 23 4392.0 24 5133.0 25 5694.0 26 6474.0 27 7122.0 28 7731.0 29 9104.0 30 10661.0 31 13152.0 32 16523.0 33 21445.0 34 28251.0 35 39009.0 36 54903.0 37 84189.0 38 93334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.010302565175934 18.87570324045591 13.114892735349027 24.999101459019133 2 13.61155237097374 19.954921080204144 43.52185926251394 22.911667286308177 3 19.396297681139213 24.31138685915514 29.501519923782 26.790795535923646 4 11.139118649220363 16.832600774359168 39.5847847289954 32.44349584742506 5 12.609015845148438 38.239371680463556 36.27059774340102 12.881014730986983 6 31.25903386922861 37.701804769249925 18.775044259365156 12.264117102156312 7 27.537832388713646 31.328370113206272 22.709588154278716 18.42420934380137 8 23.811387411019325 38.27147435558423 20.930581851644227 16.98655638175222 9 25.604635361937195 16.29234346580239 20.6569857487097 37.44603542355071 10 13.698141558929422 30.714505801560872 35.09174021501019 20.495612424499512 11 33.82439201421271 23.628840659827574 24.11265513501652 18.434112190943193 12 22.33245698804485 25.936725900856228 32.3170963290557 19.41372078204322 13 28.403566666104012 22.884560201205208 25.86592508819607 22.84594804449471 14 19.445364640149556 23.535816244068254 28.649619249978368 28.369199865803818 15 22.560450689960753 32.04709456893278 23.511836941384985 21.880617799721485 16 20.501480217000022 29.86936531080337 29.11965597351435 20.50949849868226 17 20.53068028359217 29.815580688723838 28.418298446995273 21.235440580688724 18 19.87373982470429 29.361187655873504 30.60290077688831 20.162171742533896 19 22.637571798150656 28.15268264478854 28.259245660458582 20.950499896602224 20 22.130367538301503 28.760362664235963 29.338920573831544 19.77034922363099 21 21.993104670593993 27.92566210353966 28.21873470283409 21.862498523032254 22 21.273592370261746 29.586335678943314 28.63367339403083 20.506398556764115 23 20.891015780778215 29.04051433647514 28.650584156765436 21.417885725981208 24 21.954276430312174 28.169474716997055 29.416481163028685 20.459767689662083 25 21.351617095979204 28.603018129030076 29.077976773235203 20.96738800175551 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 158.0 2 230.5 3 303.0 4 303.0 5 303.0 6 469.5 7 636.0 8 636.0 9 636.0 10 967.5 11 1299.0 12 1299.0 13 1299.0 14 2114.0 15 2929.0 16 2929.0 17 2929.0 18 4839.5 19 6750.0 20 6750.0 21 6750.0 22 10389.5 23 14029.0 24 14029.0 25 14029.0 26 19494.0 27 24959.0 28 24959.0 29 24959.0 30 32189.0 31 39419.0 32 39419.0 33 39419.0 34 44929.5 35 50440.0 36 50440.0 37 50440.0 38 54264.5 39 58089.0 40 58089.0 41 58089.0 42 60214.0 43 62339.0 44 62339.0 45 62339.0 46 63257.0 47 64175.0 48 64175.0 49 64175.0 50 60148.0 51 56121.0 52 56121.0 53 56121.0 54 47916.5 55 39712.0 56 39712.0 57 39712.0 58 32805.0 59 25898.0 60 25898.0 61 25898.0 62 20190.0 63 14482.0 64 14482.0 65 14482.0 66 10825.0 67 7168.0 68 7168.0 69 7168.0 70 5155.5 71 3143.0 72 3143.0 73 3143.0 74 2191.5 75 1240.0 76 1240.0 77 1240.0 78 859.0 79 478.0 80 478.0 81 478.0 82 324.5 83 171.0 84 171.0 85 171.0 86 110.5 87 50.0 88 50.0 89 50.0 90 33.0 91 16.0 92 16.0 93 16.0 94 10.5 95 5.0 96 5.0 97 5.0 98 5.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21644884655548877 2 0.13143043996498002 3 0.13417296920983512 4 0.07025094142590424 5 0.024471799415630303 6 0.021940233958840965 7 0.2822695484320117 8 0.2742529244855121 9 0.019619632290117404 10 0.037340590487642794 11 0.015400356528801833 12 0.016244211681064945 13 0.014978428952670274 14 0.01729903062139384 15 0.015822284104933387 16 0.020674451230446294 17 0.02004155986624896 18 0.015400356528801833 19 0.024682763203696088 20 0.016455175469130723 21 0.014978428952670274 22 0.01687710304526228 23 0.017720958197525394 24 0.01687710304526228 25 0.016666139257196504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 474015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.0247058511799 #Duplication Level Percentage of deduplicated Percentage of total 1 96.19943095780341 86.60325475027143 2 2.9287136003461565 5.273131607870256 3 0.3855286683490552 1.0412131489596237 4 0.15425352855501412 0.555465141386869 5 0.0770666554870275 0.34689514955769357 6 0.04875503391872215 0.26334945523783565 7 0.02619119124220008 0.1650498001229743 8 0.024248409129194057 0.17463647193718085 9 0.02033310177521102 0.16474333557199286 >10 0.11296435775293148 2.0510505102799903 >50 0.010553671309269543 0.6576741761270933 >100 0.010553668528007615 1.8110843960222847 >500 0.00140715580373395 0.8924520566548133 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 946 0.19957174351022647 No Hit GTCCTACAGTGGACATTTCTAAATT 788 0.16623946499583347 No Hit CTGTAGGACGTGGAATATGGCAAGA 713 0.1504171808909001 No Hit GTCCTACAGTGTGCATTTCTCATTT 675 0.14240055694440049 No Hit GGTATCAACGCAGAGTACTTTTTTT 593 0.12510152632300664 No Hit GTCCTAAAGTGTGTATTTCTCATTT 512 0.10801345948967858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCGGT 30 7.4830395E-4 19.085262 1 CCGTGAT 35 0.0021914006 16.258667 19 CCAGTAG 45 6.5720844E-4 14.829922 3 TCCAACG 65 3.418636E-6 14.591113 18 CTAACAC 60 2.4771185E-5 14.300281 3 CGGTTTC 40 0.0053303903 14.226335 13 CCGGTTT 40 0.0053303903 14.226335 12 TGCCGGT 40 0.0053303903 14.226335 10 TAGGACC 165 0.0 13.843443 4 CTAGGAC 85 2.572815E-7 13.459087 3 TCTGGAC 50 0.0014576351 13.34693 3 AAGACTG 65 5.3604075E-5 13.173708 5 GATATAC 90 5.0939525E-7 12.723508 1 GACGTGG 165 0.0 12.685793 7 GTAGGAC 355 0.0 12.621844 3 GTCCTAA 145 7.2759576E-12 12.504138 1 ACGTGGA 160 0.0 12.475691 8 TAGGACA 130 1.891749E-10 12.445787 4 TGGACAC 85 3.8612397E-6 12.31268 5 CTCCTAT 70 1.0432555E-4 12.269097 1 >>END_MODULE