FastQCFastQC Report
Thu 26 May 2016
SRR1926974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474015
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15710.33142411105133807No Hit
GGTATCAACGCAGAGTACTTTTTTT10640.2244654705019883No Hit
GTCCTACAGTGGACATTTCTAAATT8650.1824836766768984No Hit
CTGTAGGACGTGGAATATGGCAAGA8410.17742054576331973No Hit
TATCAACGCAGAGTACTTTTTTTTT8100.17088066833328058No Hit
GTCCTACAGTGTGCATTTCTCATTT7320.15442549286414986No Hit
CACACACACACACACACACACACAC6110.12889887450819068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5660.11940550404523063No Hit
CTTTAGGACGTGAAATATGGCGAGG5570.11750682995263864No Hit
CTGTAGGACCTGGAATATGGCGAGA5530.11666297480037553No Hit
GTCCTAAAGTGTGTATTTCTCATTT5500.11603008343617818No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5040.10632574918515236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAT400.005158209714.2969011
GGCGAGG1600.014.23504219
TCCCGTT500.00151356613.28043311
CCGTTTC500.00151356613.28043313
TAGACTG751.4353847E-512.6989345
TAGGACC2550.012.6989344
TGGCGAG4150.012.3484718
TGTTAGA550.002988540812.1307032
TGTTACA550.002988540812.1307032
TCCAACG550.003087225612.07821618
CCAACGA550.003087225612.07821619
GTCCTAC4200.012.027551
CCCCTGT802.7619968E-511.91408351
TGTACTG1302.4901965E-911.7220935
ATGGCGA4400.011.64685117
TTACAGT907.222243E-611.6406894
CTGTAGG5400.011.472821
TTTAGGA2500.011.4375212
TTAGGAC2500.011.4290413
CCAGGAG752.0196945E-411.429043
Produced by FastQC (version 0.11.5)