##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 474015 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.784519477231733 31.0 30.0 33.0 25.0 34.0 2 30.091933799563304 31.0 30.0 34.0 26.0 34.0 3 30.159931647732666 31.0 30.0 34.0 26.0 34.0 4 33.74517473075746 35.0 35.0 37.0 28.0 37.0 5 33.74090482368701 37.0 35.0 37.0 28.0 37.0 6 33.69556870563168 37.0 35.0 37.0 29.0 37.0 7 33.67766420893854 37.0 35.0 37.0 28.0 37.0 8 33.694754385409745 37.0 35.0 37.0 29.0 37.0 9 35.20940054639621 39.0 35.0 39.0 29.0 39.0 10 35.10667172979758 39.0 35.0 39.0 29.0 39.0 11 35.1293123635328 39.0 35.0 39.0 29.0 39.0 12 35.019393901036885 39.0 35.0 39.0 28.0 39.0 13 35.03704946046011 39.0 35.0 39.0 29.0 39.0 14 36.13344092486525 40.0 36.0 41.0 28.0 41.0 15 36.113072371127494 40.0 36.0 41.0 28.0 41.0 16 36.09503707688575 40.0 36.0 41.0 28.0 41.0 17 36.074902692952755 40.0 36.0 41.0 28.0 41.0 18 36.044243325633154 40.0 36.0 41.0 28.0 41.0 19 36.126917924538255 40.0 36.0 41.0 28.0 41.0 20 36.07889623746084 40.0 36.0 41.0 27.0 41.0 21 36.03266985221987 40.0 36.0 41.0 27.0 41.0 22 35.94322331571786 40.0 36.0 41.0 27.0 41.0 23 35.87283313819183 40.0 36.0 41.0 27.0 41.0 24 35.80444500701454 40.0 36.0 41.0 27.0 41.0 25 35.6939084206196 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3441692395068685 1101 2 -0.05862061246057593 1101 3 0.23362877854192732 1101 4 0.16833251991351972 1101 5 -0.15888037455462722 1101 6 -0.08990082942280964 1101 7 0.12875768027750922 1101 8 -0.06139592590406551 1101 9 0.14151869160269115 1101 10 -0.27237303538406366 1101 11 -0.17164788396044628 1101 12 -0.0550321431419718 1101 13 -0.07137457641330514 1101 14 0.09563245255621666 1101 15 -0.03294808323933296 1101 16 -0.028834747959109563 1101 17 0.07844957539894182 1101 18 -0.07747597947827956 1101 19 -0.14426370863832005 1101 20 -0.2868046039539891 1101 21 -0.17762803610112599 1101 22 -0.3542914928946672 1101 23 -0.40557055862749536 1101 24 -0.17311473132183863 1101 25 -0.1621343935085804 1102 1 -0.31654687016174066 1102 2 -0.2943280781150932 1102 3 0.04603655273369256 1102 4 -0.21851874303754926 1102 5 -0.32856402523680117 1102 6 -0.33753156320487676 1102 7 -0.3218960872700549 1102 8 -0.16512678041809892 1102 9 -0.33753470664709084 1102 10 -0.16332760702231042 1102 11 -0.122049140204183 1102 12 -0.08328579074666465 1102 13 -0.05116578112895098 1102 14 -0.17926950203909087 1102 15 -0.055401861834262434 1102 16 -0.19566186451674383 1102 17 -0.1191405198451676 1102 18 -0.11455963173865058 1102 19 -0.05767600436062281 1102 20 -0.285352663956985 1102 21 -0.4476494206521693 1102 22 -0.3181074121541769 1102 23 -0.23064729839092024 1102 24 -0.1692718249167271 1102 25 -0.2933850346505622 1103 1 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0.3619691000814669 2214 12 0.3545222919082178 2214 13 0.5193808896962722 2214 14 0.485491757429692 2214 15 0.4552920792217918 2214 16 0.5881201267861371 2214 17 0.4503127376303766 2214 18 0.41957995950400573 2214 19 0.4001451275552199 2214 20 0.41847392427071384 2214 21 0.4382932271289235 2214 22 0.5201821308707437 2214 23 0.27013063832016115 2214 24 0.5889942007587692 2214 25 0.4711887313159622 2215 1 0.3321458987567034 2215 2 0.032370741218635146 2215 3 -0.007371254698949059 2215 4 0.07877237055119224 2215 5 0.19870279226655185 2215 6 0.20662993131307417 2215 7 -0.1445727968029118 2215 8 0.11198478183108307 2215 9 -0.14678864613566844 2215 10 -0.15677420103072848 2215 11 -0.3188310891932957 2215 12 -0.294107899094854 2215 13 -0.33396640432899005 2215 14 -0.15090772642741968 2215 15 0.028717432308162927 2215 16 -0.006637230683082862 2215 17 -0.41242949799321593 2215 18 -0.15322454550388898 2215 19 -0.3752102240713171 2215 20 -0.39310707375096143 2215 21 -0.32130432907880646 2215 22 -0.3929931043383874 2215 23 -0.5587334278320384 2215 24 -0.6044150773962755 2215 25 -0.569839258864576 2216 1 -0.2193534493528162 2216 2 0.08973710366974075 2216 3 -2.3072408766024672 2216 4 -1.6930659606091751 2216 5 -0.3997326705756947 2216 6 -0.06846785225928897 2216 7 0.02231116669122457 2216 8 0.1758700491061873 2216 9 -0.10115631236774192 2216 10 0.1144122396472369 2216 11 -0.03591061983215127 2216 12 5.465989494766177E-4 2216 13 0.020662018096039958 2216 14 -0.11179429748348468 2216 15 -0.06124345426289324 2216 16 -0.009244792612683739 2216 17 -0.14254683828004744 2216 18 0.12056945710367728 2216 19 0.1541248476366377 2216 20 -0.2522987295527841 2216 21 -0.13877499400709326 2216 22 -0.10224994918845454 2216 23 -0.030702137088880477 2216 24 -0.25760934076002684 2216 25 -0.2321599889819126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11823.0 3 15.0 4 191.0 5 1232.0 6 1733.0 7 1306.0 8 1009.0 9 895.0 10 861.0 11 701.0 12 657.0 13 633.0 14 595.0 15 569.0 16 655.0 17 664.0 18 776.0 19 900.0 20 1138.0 21 1540.0 22 1885.0 23 2181.0 24 2748.0 25 3205.0 26 3895.0 27 4173.0 28 4975.0 29 5968.0 30 7584.0 31 10076.0 32 13036.0 33 17018.0 34 22686.0 35 31875.0 36 46801.0 37 81102.0 38 186914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.630408151195894 19.747514003531506 10.779339827479872 27.842738017792723 2 13.582749918163483 21.04961932820139 41.398718070558914 23.96891268307621 3 18.891460715408524 24.659502594215713 29.06196789038252 27.387068799993248 4 11.50301150807464 17.79753805259327 38.57219708237081 32.127253356961276 5 12.335125190929881 39.04125773213276 35.56527903189056 13.058338045046794 6 30.800841787910095 38.740028116054944 18.00570789790146 12.4534221981335 7 27.843939905469277 32.02608035111411 21.319632005401758 18.810347738014855 8 24.171565048969757 39.50452142136778 19.652886493967923 16.671027035694543 9 25.663016646623205 16.544507036310314 20.094731734075996 37.69774458299048 10 13.907981957577523 31.172546487930227 34.31963273796715 20.599838816525104 11 33.64986402752783 23.76575181490022 23.84170230362052 18.742681853951428 12 22.505242461193127 26.263359310062572 31.96698831680077 19.264409911943527 13 28.627273935524975 22.904857039785828 25.720813564184947 22.74705546050425 14 19.63672035695073 23.853675516597576 28.120629094016014 28.38897503243568 15 22.19678861437001 32.19128229687279 23.6161949025424 21.995734186214797 16 20.638798350263176 30.300728879887767 28.628629895678408 20.43184287417065 17 20.392603609590417 30.11233821714503 28.266405071569466 21.228653101695095 18 19.926373637965046 29.403710853032077 30.5705515648239 20.099363944178982 19 22.055302280739138 28.11125433268708 28.51651747947841 21.316925907095374 20 21.95425019988903 28.780012362530144 29.493283939470018 19.772453498110814 21 21.733158347821682 27.830503181810514 28.318324527792317 22.118013942575494 22 21.286457179625117 29.48915118719872 28.572724491840972 20.65166714133519 23 20.955710168897966 28.80590024767201 28.848093094296797 21.39029648913323 24 21.163465291182767 28.388553104859547 29.856861069797368 20.59112053416031 25 21.27620072365159 28.467989493338397 29.292488158907982 20.96332162410203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 230.0 3 231.0 4 231.0 5 231.0 6 396.5 7 562.0 8 562.0 9 562.0 10 913.5 11 1265.0 12 1265.0 13 1265.0 14 1980.0 15 2695.0 16 2695.0 17 2695.0 18 4503.5 19 6312.0 20 6312.0 21 6312.0 22 9957.5 23 13603.0 24 13603.0 25 13603.0 26 18939.0 27 24275.0 28 24275.0 29 24275.0 30 31635.0 31 38995.0 32 38995.0 33 38995.0 34 44497.0 35 49999.0 36 49999.0 37 49999.0 38 53900.0 39 57801.0 40 57801.0 41 57801.0 42 60332.0 43 62863.0 44 62863.0 45 62863.0 46 63911.5 47 64960.0 48 64960.0 49 64960.0 50 61032.0 51 57104.0 52 57104.0 53 57104.0 54 48818.5 55 40533.0 56 40533.0 57 40533.0 58 33376.5 59 26220.0 60 26220.0 61 26220.0 62 20432.0 63 14644.0 64 14644.0 65 14644.0 66 10906.0 67 7168.0 68 7168.0 69 7168.0 70 5047.0 71 2926.0 72 2926.0 73 2926.0 74 2023.5 75 1121.0 76 1121.0 77 1121.0 78 739.0 79 357.0 80 357.0 81 357.0 82 232.0 83 107.0 84 107.0 85 107.0 86 69.5 87 32.0 88 32.0 89 32.0 90 22.5 91 13.0 92 13.0 93 13.0 94 6.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11877261268103328 2 0.10759153191354705 3 0.016033247892999168 4 0.0 5 0.004008311973249792 6 0.35589591046696833 7 0.02004155986624896 8 0.010337225615223147 9 0.009493370462960034 10 0.0044302395493813485 11 0.0046412033374471275 12 2.1096378806577848E-4 13 4.2192757613155696E-4 14 0.0 15 0.002953493032920899 16 0.0 17 0.0 18 0.0 19 4.2192757613155696E-4 20 4.2192757613155696E-4 21 0.014978428952670274 22 0.0 23 2.1096378806577848E-4 24 0.0 25 0.006328913641973355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 474015.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.39798256771392 #Duplication Level Percentage of deduplicated Percentage of total 1 94.93711018465247 82.97311900947392 2 3.9638142078878005 6.928587300852696 3 0.5163030016105975 1.3537152220326414 4 0.17902346571974884 0.6258515894474537 5 0.09914157959653762 0.43323870226569083 6 0.05559470579002716 0.29153190764963866 7 0.03715136526472406 0.2272868061641188 8 0.03006684371199707 0.2102225186085839 9 0.018968270387327218 0.14920097081861777 >10 0.13455369838775336 2.317886434292743 >50 0.013532224437872857 0.7995822467733098 >100 0.01184069139521853 1.7417201833894196 >500 0.0024164676316768227 1.3915598749062208 >1k 4.8329352633536454E-4 0.5564972333249191 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1571 0.33142411105133807 No Hit GGTATCAACGCAGAGTACTTTTTTT 1064 0.2244654705019883 No Hit GTCCTACAGTGGACATTTCTAAATT 865 0.1824836766768984 No Hit CTGTAGGACGTGGAATATGGCAAGA 841 0.17742054576331973 No Hit TATCAACGCAGAGTACTTTTTTTTT 810 0.17088066833328058 No Hit GTCCTACAGTGTGCATTTCTCATTT 732 0.15442549286414986 No Hit CACACACACACACACACACACACAC 611 0.12889887450819068 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 566 0.11940550404523063 No Hit CTTTAGGACGTGAAATATGGCGAGG 557 0.11750682995263864 No Hit CTGTAGGACCTGGAATATGGCGAGA 553 0.11666297480037553 No Hit GTCCTAAAGTGTGTATTTCTCATTT 550 0.11603008343617818 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 504 0.10632574918515236 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.21927576131557E-4 0.0 10 0.0 0.0 0.0 4.21927576131557E-4 0.0 11 0.0 0.0 0.0 4.21927576131557E-4 0.0 12 0.0 0.0 0.0 4.21927576131557E-4 0.0 13 0.0 0.0 0.0 4.21927576131557E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATTAT 40 0.0051582097 14.296901 1 GGCGAGG 160 0.0 14.235042 19 TCCCGTT 50 0.001513566 13.280433 11 CCGTTTC 50 0.001513566 13.280433 13 TAGACTG 75 1.4353847E-5 12.698934 5 TAGGACC 255 0.0 12.698934 4 TGGCGAG 415 0.0 12.34847 18 TGTTAGA 55 0.0029885408 12.130703 2 TGTTACA 55 0.0029885408 12.130703 2 TCCAACG 55 0.0030872256 12.078216 18 CCAACGA 55 0.0030872256 12.078216 19 GTCCTAC 420 0.0 12.02755 1 CCCCTGT 80 2.7619968E-5 11.9140835 1 TGTACTG 130 2.4901965E-9 11.722093 5 ATGGCGA 440 0.0 11.646851 17 TTACAGT 90 7.222243E-6 11.640689 4 CTGTAGG 540 0.0 11.47282 1 TTTAGGA 250 0.0 11.437521 2 TTAGGAC 250 0.0 11.429041 3 CCAGGAG 75 2.0196945E-4 11.42904 3 >>END_MODULE