FastQCFastQC Report
Thu 26 May 2016
SRR1926973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540214
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11700.21658083648331958No Hit
GTCCTACAGTGTGCATTTCTCATTT9720.17992869492460395No Hit
GTATCAACGCAGAGTACTTTTTTTT9650.17863291214222513No Hit
CTGTAGGACGTGGAATATGGCAAGA9320.17252422188243918No Hit
GTCCTAAAGTGTGTATTTCTCATTT7240.13402096206318237No Hit
CTGTAGGACCTGGAATATGGCGAGA6080.11254799024090452No Hit
CTTTAGGACGTGAAATATGGCGAGG6020.11143731928457982No Hit
GGTATCAACGCAGAGTACTTTTTTT5610.10384773441636092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT250.00609400618.96371312
TCCAACG607.826566E-817.38340418
CCAACGA402.7974267E-416.5932519
CTAACCT456.6373206E-414.8112524
CTACTAG500.00144946813.3574421
GTCTTAC500.00144946813.3574421
TAGGACC2050.013.0050024
GGACGTG4250.012.9880366
GACGTGG2400.012.6894627
AGGACGT4500.012.6894615
GTCTAGG1301.8189894E-1012.4767311
GGCGAGG1850.012.30078719
GTATAGC1404.9112714E-1112.2670381
TAATACC701.06559826E-412.2419534
TACACCC701.07023276E-412.2362665
TGTAGGA6650.012.190762
GTCCTAA2900.012.17303851
CCTAACC550.002974915612.1386073
TAGGACG4800.012.1002644
GTAGGAC6000.012.0808043
Produced by FastQC (version 0.11.5)