##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 540214 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.695185611627984 30.0 27.0 31.0 10.0 33.0 2 27.319758466089365 31.0 28.0 31.0 10.0 34.0 3 27.123969389908442 31.0 27.0 31.0 10.0 34.0 4 30.729533110952325 35.0 32.0 37.0 10.0 37.0 5 30.506504829567543 35.0 31.0 37.0 10.0 37.0 6 30.168442506117945 35.0 30.0 37.0 10.0 37.0 7 30.229479428522772 35.0 30.0 37.0 10.0 37.0 8 29.90278482231116 35.0 30.0 37.0 8.0 37.0 9 31.94456456145157 37.0 32.0 39.0 8.0 39.0 10 32.064661411958966 37.0 32.0 39.0 8.0 39.0 11 32.24393295990107 37.0 32.0 39.0 7.0 39.0 12 32.09400533862507 37.0 32.0 39.0 2.0 39.0 13 32.217698911912684 37.0 32.0 39.0 2.0 39.0 14 33.14121440762364 38.0 33.0 40.0 2.0 41.0 15 32.716145823692095 38.0 32.0 40.0 2.0 41.0 16 32.79117534902835 38.0 32.0 40.0 2.0 41.0 17 33.009416638591375 38.0 32.0 40.0 2.0 41.0 18 33.06043901120667 38.0 33.0 40.0 2.0 41.0 19 32.44313549815443 38.0 32.0 40.0 2.0 41.0 20 32.72425742390978 39.0 32.0 40.0 2.0 41.0 21 32.72565131595997 39.0 32.0 40.0 2.0 41.0 22 32.74014372082175 39.0 32.0 40.0 2.0 41.0 23 32.71068132258697 39.0 33.0 40.0 2.0 41.0 24 31.38445690041354 38.0 30.0 40.0 2.0 41.0 25 31.96685572754501 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.0218352425209645 1101 2 -1.1169050709457906 1101 3 -1.9437515322009915 1101 4 -1.411458493157891 1101 5 -1.2794813917931052 1101 6 -3.160916182922353 1101 7 -1.2797692281075932 1101 8 -0.8142369768433468 1101 9 -1.219932946498318 1101 10 -9.15114699147967 1101 11 -4.75693448514793 1101 12 -2.6873997173192308 1101 13 -1.9585510895292195 1101 14 -1.889932284237684 1101 15 -2.9228232514807786 1101 16 -1.4800250420452663 1101 17 -1.4941683941149826 1101 18 -1.0684237264929841 1101 19 -1.8006802978928533 1101 20 -1.2851675268237273 1101 21 -1.5483127034214839 1101 22 -1.0920320210574275 1101 23 -1.0422385809296166 1101 24 -1.1889195167528328 1101 25 -1.078889409901148 1102 1 -0.9438184054651941 1102 2 -0.6931972377965039 1102 3 -0.862979972339204 1102 4 -0.5178062294169301 1102 5 -0.5196795656018622 1102 6 -1.6010959523330577 1102 7 -0.45743400683923596 1102 8 -0.02446269788016764 1102 9 -0.14487565538823688 1102 10 -0.054179561327330816 1102 11 -0.08916058614651945 1102 12 -0.08703637888016047 1102 13 -0.42801759946165774 1102 14 -0.5159756948694891 1102 15 -0.01422780389768974 1102 16 -0.043110569064111814 1102 17 -0.4145159403931373 1102 18 -0.5217626405147584 1102 19 -0.17316453541645416 1102 20 -0.2359179513122598 1102 21 -0.3259044582511521 1102 22 -0.24639148863600013 1102 23 -0.7453222081396298 1102 24 -3.8475654280080107 1102 25 -2.1287653286368275 1103 1 -0.17601060228130194 1103 2 -0.13819200544890364 1103 3 0.20895268863033678 1103 4 0.2899983801815047 1103 5 -0.13148927353136486 1103 6 0.9203709299006277 1103 7 0.45734716127167374 1103 8 0.7303697386829633 1103 9 0.6671861176790053 1103 10 0.5299457526848101 1103 11 0.3230999305870128 1103 12 0.13983507645383852 1103 13 0.3027945122111859 1103 14 0.40581567845779887 1103 15 0.7057275429342411 1103 16 0.5538646492830424 1103 17 0.18106208431723303 1103 18 0.02713343394140111 1103 19 -2.003318540372213 1103 20 -0.3620545968499371 1103 21 0.03048684721244399 1103 22 0.30604034114589496 1103 23 0.35483519000067787 1103 24 1.8303301132334404 1103 25 1.2340887102507807 1104 1 -0.823949670381662 1104 2 -0.5373386174987402 1104 3 -1.6444267276523057 1104 4 -0.8145501775928601 1104 5 -0.5920968857518822 1104 6 -2.710543048593994 1104 7 -0.7983193347174016 1104 8 -0.23349781550717097 1104 9 -0.17153209361985589 1104 10 0.05674213431990438 1104 11 -0.3952607111607236 1104 12 -0.45106354105768176 1104 13 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1107 11 -0.16187659922210784 1107 12 -0.1087762469931377 1107 13 -0.24334748669033246 1107 14 -0.12289071483351677 1107 15 -1.994815187535103 1107 16 -0.6935958137790124 1107 17 -0.5555027177574345 1107 18 -0.702516296816718 1107 19 0.3553110722705455 1107 20 0.41154441111689977 1107 21 0.192108413741515 1107 22 -0.37827851371263677 1107 23 -0.5071592383062438 1107 24 -4.260351456579762 1107 25 -2.2451788159514656 1108 1 0.11651668144832783 1108 2 -0.3742532180497058 1108 3 0.10175587762120841 1108 4 -0.0847390795999452 1108 5 0.18251940704622882 1108 6 -3.143256547216584 1108 7 -1.8813378313722637 1108 8 -0.2961896691081165 1108 9 -0.4344403974372497 1108 10 -0.053253930221615065 1108 11 -0.3023346977801644 1108 12 -0.039837645740878 1108 13 -0.1803288594752388 1108 14 -0.11438704702351998 1108 15 -1.399615794080649 1108 16 -0.3323836739154302 1108 17 -0.428080592274128 1108 18 -0.5262893570180438 1108 19 -0.49128718083352396 1108 20 -1.9629694851548578 1108 21 -1.043372821955142 1108 22 -0.35202191808938466 1108 23 -0.4625426097818135 1108 24 -1.4704545805131346 1108 25 -0.7124482978698055 1109 1 0.33039164698361034 1109 2 0.11951909564639607 1109 3 -0.9472639104296086 1109 4 0.11620260256111692 1109 5 0.4237286061215144 1109 6 0.5347164324439291 1109 7 0.7880935537912954 1109 8 1.3233028688384252 1109 9 0.3881628023025847 1109 10 0.7251481793726526 1109 11 0.291900680341989 1109 12 0.09700460835777491 1109 13 0.002005409413193604 1109 14 0.29314782420215124 1109 15 -1.0623620842629755 1109 16 0.2730628120971943 1109 17 0.15613845487360578 1109 18 0.4354565726103061 1109 19 -1.4456396329540766 1109 20 0.08177172899299734 1109 21 0.29792616213099166 1109 22 0.5094843491871544 1109 23 0.28042057836373857 1109 24 -0.7155945249454021 1109 25 -0.3046617458263121 1110 1 0.48181042463021484 1110 2 0.537019486788104 1110 3 0.9768887343994983 1110 4 0.8378590521322842 1110 5 0.8429557101083169 1110 6 1.0000072285239767 1110 7 1.046500255826654 1110 8 1.254422103054079 1110 9 1.0857860830569912 1110 10 0.9581107577787904 1110 11 0.6908296632313125 1110 12 0.5393702334022201 1110 13 0.4962591821527482 1110 14 0.5313431674823406 1110 15 1.060106517725444 1110 16 0.8883941446814347 1110 17 0.3776512487632644 1110 18 0.6899902605787815 1110 19 -1.359203005022291 1110 20 0.5055374991154622 1110 21 0.5550954340384848 1110 22 0.6353878865305234 1110 23 0.3849307978297958 1110 24 -0.1499339221604714 1110 25 0.4272497281378733 1111 1 1.0029021814701906 1111 2 0.6053862502261254 1111 3 1.254614277638698 1111 4 1.115939240396557 1111 5 1.2560181936131976 1111 6 1.325799543998599 1111 7 1.383459765811697 1111 8 1.8023156765563222 1111 9 1.1744665836288242 1111 10 1.0028400179177837 1111 11 0.9205461671461563 1111 12 0.9359073584132211 1111 13 0.9019208562972025 1111 14 0.877515200560552 1111 15 -0.18976976889371144 1111 16 0.18330760029856918 1111 17 0.41075145110337274 1111 18 0.7570841507872856 1111 19 1.2236079829252802 1111 20 0.6355932526124235 1111 21 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2215 3 0.21048878694062623 2215 4 0.4384452141122104 2215 5 0.6779491971974281 2215 6 1.00716048443212 2215 7 0.5504517866343619 2215 8 -0.4424839906223781 2215 9 0.09720830576570805 2215 10 -0.29540840331450724 2215 11 -0.14314857598407116 2215 12 -0.3229145395215838 2215 13 -0.10623433230153267 2215 14 -0.09567775045498195 2215 15 -0.23782084416252758 2215 16 0.19300955455697988 2215 17 -0.14258529578176393 2215 18 -0.8763483888952592 2215 19 0.2544472899742587 2215 20 -3.720605544110562 2215 21 -2.100081509162365 2215 22 -0.8852892372804604 2215 23 -0.863930811023387 2215 24 -0.702354645257774 2215 25 -0.6912825135075416 2216 1 -0.3455164618922737 2216 2 -1.5184693469440766 2216 3 -0.5699929841174409 2216 4 -0.6290409926454714 2216 5 -0.36878673772263326 2216 6 -1.83416976519824 2216 7 -9.537511416044318 2216 8 -10.763032325940557 2216 9 -6.302502309421531 2216 10 -3.587541796480881 2216 11 -2.5232480484177238 2216 12 -2.359218525898058 2216 13 -2.0653206126998818 2216 14 -2.183944433230547 2216 15 -3.3734074093431516 2216 16 -2.358059961083036 2216 17 -2.895844470661089 2216 18 -2.3297994322339406 2216 19 -1.6725894785811732 2216 20 -2.649558120670971 2216 21 -4.882605658123627 2216 22 -3.7167268987289965 2216 23 -3.288674938467768 2216 24 -3.4995666555904776 2216 25 -2.916247562956098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43147.0 3 2.0 4 371.0 5 1907.0 6 2376.0 7 1937.0 8 1499.0 9 1397.0 10 1256.0 11 1295.0 12 1238.0 13 1149.0 14 1228.0 15 1164.0 16 1299.0 17 1465.0 18 1592.0 19 1916.0 20 2564.0 21 3369.0 22 3944.0 23 4792.0 24 5509.0 25 6183.0 26 7197.0 27 7624.0 28 8606.0 29 10111.0 30 11956.0 31 14497.0 32 18245.0 33 23898.0 34 31859.0 35 43638.0 36 61695.0 37 96547.0 38 111742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97684703385664 18.78263740870801 12.968703333722894 25.271812223712452 2 13.436456879567487 20.155612022678284 43.892005701078865 22.51592539667536 3 19.220282876360677 24.500814599400588 29.60841771248626 26.67048481175247 4 10.758219306223685 16.948191835986197 39.94780099174411 32.34578786604601 5 12.249950008517068 38.650266991549586 36.648385830562205 12.451397169371143 6 30.904849191801365 38.07235831065193 19.15754781610472 11.865244681441983 7 27.499819011748457 31.351620262516498 22.91626833782246 18.232292387912583 8 23.651834307225116 38.70662964145438 21.107526562372385 16.534009488948122 9 25.87704681285965 15.969583861005576 20.717708310189813 37.43566101594496 10 13.326148627128562 30.855535556888803 35.72613677606678 20.09217903991586 11 33.743735200075534 23.531949322466634 24.67706310252703 18.047252374930803 12 22.452070392416427 26.022791442563157 32.758579191468485 18.766558973551927 13 28.472800790926563 23.08109589243991 26.027445447914005 22.41865786871952 14 19.040654079293784 23.745551897918602 29.06124217300664 28.15255184978097 15 22.351470942180587 32.82820802399422 23.43528409827264 21.385036935552552 16 20.084761924158627 30.06826500969263 29.79461248914555 20.05236057700319 17 20.047249284410327 30.242504360190626 28.93204027297941 20.778206082419636 18 19.45324375012728 29.41712288315242 31.405899679892467 19.723733686827824 19 22.52542664738568 28.08494249957877 28.51969049201697 20.869940361018585 20 21.992416471956126 28.93552890294343 29.633154236666805 19.438900388433638 21 21.835894758474364 28.14660942108274 28.094584812146728 21.92291100829617 22 20.987002888247055 29.92316522254314 28.87580537658298 20.214026512626823 23 20.703336906672888 29.298607138162343 29.034587334825616 20.96346862033915 24 21.84782467827011 28.220585158675643 29.857792704308434 20.073797458745812 25 21.045459763092317 28.792726141678056 29.530516953451603 20.63129714177803 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 168.0 2 233.5 3 299.0 4 299.0 5 299.0 6 479.5 7 660.0 8 660.0 9 660.0 10 991.0 11 1322.0 12 1322.0 13 1322.0 14 2185.0 15 3048.0 16 3048.0 17 3048.0 18 5204.5 19 7361.0 20 7361.0 21 7361.0 22 11625.0 23 15889.0 24 15889.0 25 15889.0 26 22518.0 27 29147.0 28 29147.0 29 29147.0 30 38211.5 31 47276.0 32 47276.0 33 47276.0 34 53832.5 35 60389.0 36 60389.0 37 60389.0 38 64884.0 39 69379.0 40 69379.0 41 69379.0 42 71555.0 43 73731.0 44 73731.0 45 73731.0 46 74181.0 47 74631.0 48 74631.0 49 74631.0 50 68901.5 51 63172.0 52 63172.0 53 63172.0 54 52932.0 55 42692.0 56 42692.0 57 42692.0 58 34469.5 59 26247.0 60 26247.0 61 26247.0 62 20175.5 63 14104.0 64 14104.0 65 14104.0 66 10340.0 67 6576.0 68 6576.0 69 6576.0 70 4591.5 71 2607.0 72 2607.0 73 2607.0 74 1781.0 75 955.0 76 955.0 77 955.0 78 658.0 79 361.0 80 361.0 81 361.0 82 239.0 83 117.0 84 117.0 85 117.0 86 86.5 87 56.0 88 56.0 89 56.0 90 36.0 91 16.0 92 16.0 93 16.0 94 11.0 95 6.0 96 6.0 97 6.0 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21232326448407482 2 0.12346958797809758 3 0.12680160084707173 4 0.06608492190132059 5 0.02258364277860255 6 0.022953866430710793 7 0.2785932982114495 8 0.2743357262122048 9 0.019621853561736645 10 0.03294990503763324 11 0.017215399823033096 12 0.01832607077935781 13 0.016289840692762497 14 0.017770735301195453 15 0.015549393388546022 16 0.02091763634411548 17 0.01851118260541193 18 0.0153642815624919 19 0.024249649213089627 20 0.017770735301195453 21 0.01573450521460014 22 0.01740051164908721 23 0.019066518083574287 24 0.01610472886670838 25 0.016289840692762497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 540214.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.80614407500738 #Duplication Level Percentage of deduplicated Percentage of total 1 95.96520621203976 85.22299929054198 2 3.033676986319107 5.388183110481777 3 0.43255659374230937 1.1524104955342191 4 0.16508517780733692 0.586423123400263 5 0.09113108789597138 0.4046500260700899 6 0.05440266146751533 0.2898774355408812 7 0.03967549603595923 0.2466399472051746 8 0.03415416884990455 0.2426480031717402 9 0.020516211263971303 0.16397690520433722 >10 0.138123470200908 2.4181190563125745 >50 0.012735981549534618 0.7698440239387013 >100 0.010856877820363986 1.809463963899464 >500 0.0016702888954221267 1.0878291911192328 >1k 2.0878611192776583E-4 0.21693542757959816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1170 0.21658083648331958 No Hit GTCCTACAGTGTGCATTTCTCATTT 972 0.17992869492460395 No Hit GTATCAACGCAGAGTACTTTTTTTT 965 0.17863291214222513 No Hit CTGTAGGACGTGGAATATGGCAAGA 932 0.17252422188243918 No Hit GTCCTAAAGTGTGTATTTCTCATTT 724 0.13402096206318237 No Hit CTGTAGGACCTGGAATATGGCGAGA 608 0.11254799024090452 No Hit CTTTAGGACGTGAAATATGGCGAGG 602 0.11143731928457982 No Hit GGTATCAACGCAGAGTACTTTTTTT 561 0.10384773441636092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.851118260541193E-4 0.0 10 0.0 0.0 0.0 1.851118260541193E-4 0.0 11 0.0 0.0 0.0 1.851118260541193E-4 0.0 12 0.0 0.0 0.0 1.851118260541193E-4 0.0 13 0.0 0.0 0.0 1.851118260541193E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTTT 25 0.006094006 18.963713 12 TCCAACG 60 7.826566E-8 17.383404 18 CCAACGA 40 2.7974267E-4 16.59325 19 CTAACCT 45 6.6373206E-4 14.811252 4 CTACTAG 50 0.001449468 13.357442 1 GTCTTAC 50 0.001449468 13.357442 1 TAGGACC 205 0.0 13.005002 4 GGACGTG 425 0.0 12.988036 6 GACGTGG 240 0.0 12.689462 7 AGGACGT 450 0.0 12.689461 5 GTCTAGG 130 1.8189894E-10 12.476731 1 GGCGAGG 185 0.0 12.300787 19 GTATAGC 140 4.9112714E-11 12.267038 1 TAATACC 70 1.06559826E-4 12.241953 4 TACACCC 70 1.07023276E-4 12.236266 5 TGTAGGA 665 0.0 12.19076 2 GTCCTAA 290 0.0 12.1730385 1 CCTAACC 55 0.0029749156 12.138607 3 TAGGACG 480 0.0 12.100264 4 GTAGGAC 600 0.0 12.080804 3 >>END_MODULE