FastQCFastQC Report
Thu 26 May 2016
SRR1926973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540214
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16040.29691936899080734No Hit
CTGTAGGACGTGGAATATGGCAAGA11700.21658083648331958No Hit
GTCCTACAGTGGACATTTCTAAATT11690.21639572465726545No Hit
GGTATCAACGCAGAGTACTTTTTTT11010.20380812048558533No Hit
GTCCTACAGTGTGCATTTCTCATTT10450.19344185822655466No Hit
TATCAACGCAGAGTACTTTTTTTTT9090.16826664988319445No Hit
CTGTAGGACCTGGAATATGGCGAGA8020.14845968449540367No Hit
GTCCTAAAGTGTGTATTTCTCATTT7800.14438722432221304No Hit
CTTTAGGACGTGAAATATGGCGAGG7590.14049987597507654No Hit
CACACACACACACACACACACACAC7060.13068894919420823No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5930.10977131285009276No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5920.10958620102403863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG350.002121735116.3380721
TAGGACC2600.015.3797614
ACTGGTC653.3961333E-614.6012838
GTAGGAC6000.013.9638463
GGCGAGG1850.013.84764219
TGTAGGA6550.013.3876532
GTCCTTA500.001461541313.3427581
CTGTAGG6550.013.09541
AGGACGT4750.013.0284695
GGACGTG4500.012.904786
GGACCTG3950.012.7735636
GGACTGT905.2480027E-712.6932276
GACAGTA604.1177403E-412.6544467
TAGGACG4900.012.6296384
AGGACCT4300.012.6206015
TAGGACT1301.891749E-1012.4502834
CTAGGAC1155.120455E-912.418443
GTCTTAC701.0562159E-412.2535531
GGACCAT701.06727326E-412.2398986
TGGCGAG5900.012.22208718
Produced by FastQC (version 0.11.5)