FastQCFastQC Report
Thu 26 May 2016
SRR1926972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699199
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20680.2957670133967583No Hit
CTGTAGGACGTGGAATATGGCAAGA16920.2419911927791659No Hit
GTCCTACAGTGTGCATTTCTCATTT16030.22926234162234213No Hit
GTCCTAAAGTGTGTATTTCTCATTT14680.2099545336878342No Hit
CTGTAGGACCTGGAATATGGCGAGA11470.16404485704355984No Hit
CTTTAGGACGTGAAATATGGCGAGG11270.16118444105326238No Hit
GTATCAACGCAGAGTACTTTTTTTT8070.11541778520850288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.014.7876124
TCGAACT551.9895151E-413.79196519
GTGCTAC903.4327968E-813.7799641
GTAGGAC10400.013.6570383
AGGACCT6750.013.3957835
GAGTATA500.001451427213.3559661
CTGTAGG10400.013.3009271
TGTAGGA11250.013.2192542
GGACCTG6350.013.1903786
TTATAGT604.0123038E-412.6943884
TTAACCT604.0123038E-412.6943884
GTCCTAC10250.012.3786991
ACCATCC853.8828894E-612.3086488
TAGGACA2500.012.1866134
CCTAGTA550.002986650212.1330552
GGACGTG8150.012.0289556
CTACACT1900.012.0262634
AGGACGT8350.011.9688155
CTCGAAC658.142418E-411.67012318
GTCCTAG907.1038794E-611.659971
Produced by FastQC (version 0.11.5)