FastQCFastQC Report
Thu 26 May 2016
SRR1926972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699199
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20890.29877045018657067No Hit
CTGTAGGACGTGGAATATGGCAAGA20070.287042744626351No Hit
GTCCTACAGTGTGCATTTCTCATTT16860.24113306798207662No Hit
GTCCTAAAGTGTGTATTTCTCATTT15430.22068109365144975No Hit
CTTTAGGACGTGAAATATGGCGAGG14350.2052348473038434No Hit
GTATCAACGCAGAGTACTTTTTTTT13600.1945082873402279No Hit
CTGTAGGACCTGGAATATGGCGAGA13540.19365016254313866No Hit
GGTATCAACGCAGAGTACTTTTTTT8360.1195653883944342No Hit
GTACATGGGAAGCAGTGGTATCAAC7330.10483424604440224No Hit
TATCAACGCAGAGTACTTTTTTTTT7110.10168778845507502No Hit
CTGAAGGACCTGGAATATGGCGAGA7090.10140174685604526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA350.00212480316.3355122
TAGGACC4500.015.4393854
TAGACTC400.00521147914.2761445
CCAACGA602.5910951E-514.23428419
TGCGCCA400.00531401814.23428312
TCCAACG759.771629E-713.91796718
TAGGACT1351.8189894E-1213.39494
ATCCCGT500.001509802513.286283510
CCCGTTT500.001510616613.28533412
GGACCTG5650.013.1391066
GTAGGAC10550.012.5404513
CTGTAGG11050.012.5050791
AGGACCT6650.012.4513745
GACGTGA4500.012.446277
GTCCTAT1155.0604285E-912.4300851
TGTAGGA11350.012.3415872
GTTACAC701.0700406E-412.2375733
GTCTTAC550.00299460512.1287511
TGAATAG550.003023730612.1130925
GACAGTC550.00308271112.0818967
Produced by FastQC (version 0.11.5)